Abstract
Objective
To explore how arylamine N-acetyltransferases (NATs) is related to cell apoptosis.
Methods
NAT activity in apoptotic HepG2 cells was measured using high performance liquid chromatography (HPLC); the apoptosis rate of HepG2 cells acted upon by an NAT inhibitor was measured using flow cytometry.
Results
NAT activity was lowered in apoptotic HepG2 cells; apoptosis rate induced by camptothecin (CAM) increased after inhibition of NAT activity in HepG2 cells.
Conclusion
NAT can inhibit apoptosis in HepG2 cells.
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Project supported by the National Natural Science Foundation of China (No. 30400591), the Science Foundation of Heilongjiang Province, China (Nos. D2004-13 and D200505), and the Young Scientist Fund of Harbin City, China (No. 2004AFQXJ035)
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Ji, Yb., Gao, Sy. Arylamine N-acetyltransferases: a new inhibitor of apoptosis in HepG2 cells. J. Zhejiang Univ. Sci. B 9, 701–706 (2008). https://doi.org/10.1631/jzus.B0820090
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DOI: https://doi.org/10.1631/jzus.B0820090