Abstract
Membrane fouling restricts the wide application of anaerobic membrane bio-reactors (AnMBRs). In this study, a microbial electrolytic cell (MEC)-AnMBR biosystem was constructed to relieve membrane fouling. Total chemical oxygen demand (COD) removal efficiency and methane production in MEC-AnMBR were increased to 6.7% and 77.1%, respectively, in comparison to AnMBR. The membrane fouling of MEC-AnMBR was greatly lessened by the slower growth of extracellular polymeric substances (EPS) and soluble microbial products (SMP). High-throughput sequencing analysis showed that Synergistaceae-uncultured and Thermovirga were enriched in MEC-AnMBR, and Thermovirga was found as the key functional microorganism. These results indicated that MEC-AnMBR could simultaneously enhance the reactor efficiency and mitigate membrane fouling.
中文概要
目的:将微生物电解池(MEC)与厌氧膜生物反应器 (AnMBR)耦合,构建MEC-AnMBR 系统,以 期同步实现污水高效处理和膜污染缓解,推动膜 生物反应器的理论创新和技术创新。
创新点:1. 将MEC 与AnMBR 耦合,构建MEC-AnMBR 系统用于高浓度有机废水的处理;2. 研究反应器 运行和微生物群落之间的关系;3. 探究膜污染运 行周期中各膜污染阶段微生物代谢产物与自身 代谢活性的变化规律。
方法:1. 启动和运行MEC-AnMBR 反应器,并与传统 AnMBR 对照,综合考察MEC-AnMBR 反应器的 运行性能; 2. 利用高通量测序技术对传统 AnMBR 和MEC-AnMBR 各膜污染阶段的阴极膜 表面微生物群落结构及多样性进行研究,并综合 分析MEC-AnMBR 反应器的运行特性与微生物 群落间的相互关系;3. 对MEC-AnMBR 反应器阴 极膜组件及微生物分泌物进行原位观察,并研究 其在膜污染运行周期中各膜污染阶段微生物代 谢产物与自身代谢活性的变化规律。
结论:1. 成功构建微生物电解池MEC-AnMBR 生物系 统;2. 与AnMBR 相比,MEC-AnMBR 中的化学 需氧量(COD)去除效率和甲烷产量分别增加 6.7%和77.1%;3. 与AnMBR 相比,MEC-AnMBR 的膜污染因细胞外聚合物和可溶性微生物产物 增长缓慢而大大减少;4. 高通量测序分析表明 MEC-AnMBR 富含互养菌属(Synergistaceaeuncultured) 和互营热菌属(Thermovirga),而 Thermovirga 是关键的功能性微生物;5. 这些结 果表明MEC-AnMBR 可同时提高反应器效率并 减轻膜污染。
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Project supported by the Zhejiang Provincial Key Science and Technology Project (No. 2015C03008), China
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Shu-wen DU wrote the first draft of the manuscript and edited the final version. Chao SUN formulated research goals and revised the manuscript. A-qiang DING provided experimental resources. Wei-wang CHEN conducted a research and investigation process. Ming-jie ZHANG created models. Ran CHENG analyzed the data. Dong-lei WU oversaw and led the research activity.
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Shu-wen DU, Chao SUN, A-qiang DING, Wei-wang CHEN, Ming-jie ZHANG, Ran CHENG, and Dong-lei WU declare that they have no conflict of interest.
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Du, Sw., Sun, C., Ding, Aq. et al. Microbial dynamics and performance in a microbial electrolysis cell-anaerobic membrane bioreactor. J. Zhejiang Univ. Sci. A 20, 533–545 (2019). https://doi.org/10.1631/jzus.A1900009
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DOI: https://doi.org/10.1631/jzus.A1900009
Key words
- Microbial electrolytic cell-anaerobic membrane bio-reactor (MEC-AnMBR)
- Chemical oxygen demand (COD) removal efficiency
- Methane production
- Membrane fouling
- Microbial mechanism