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Microbial and hydrodynamic properties of aerobic granules in a sequencing batch reactor treating landfill leachate

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Abstract

A sequencing batch reactor (SBR) seeded with activated sludge was established for landfill leachate treatment. Small bio-aggregates began to appear after 40-d operation, and gradually changed to mature aerobic granules, with a mean size of 0.36–0.60 mm. Their sludge volume index at 5 min (SVI5 min), mixed liquor volatile suspended solids (MLVSS), and wet density were around 35 ml/g, 3.4 g/L, and 1.062 g/cm3, respectively. The settling velocities of the granules in distilled water ranged from 0.3 to 1.3 cm/s, which were faster than those in landfill leachate with a salt content of 1.4% (w/v), and also slightly faster than those predicted by Stokes’ law for porous but impermeable particles. Microbial community evolution during the granulation process and stages under different nitrogen loading rates (NLRs) were monitored and analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), cloning, and sequencing of 16S ribosomal RNA (rRNA) fragments. Results revealed that some primary and dominant communities in inoculating activated sludge died out gradually; while a few common bacteria, inhabiting soils, municipal wastewater, or activated sludge systems, dominated in the SBR system throughout. In addition, some other dominant species, associated with the aerobic granulation process, were thought to play a significant role in the formation and growth of aerobic granular sludge. During the stable operation time under low NLR, a few species were present in abundance, and may have been responsible for the high organic removal efficiency at this time.

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References

  • Adav, S.S., Chen, M.Y., Lee, D.J, Ren, N.Q., 2007. Degradation of phenol by Acinetobacter strain isolated from aerobic granules. Chemosphere, 67(8):1566–1572. [doi:10.1016/j.chemosphere.2006.11.067]

    Article  Google Scholar 

  • APHA, 1998. Standard Methods for the Examination of Water and Wastewater, 20th Edition. American Public Health Association, Washington DC, USA.

    Google Scholar 

  • Bao, R.L., Yu, S.L., Zuo, X.T., Wang, J., 2009. Shut-cut nitrification characteristics of aerobic granule in a sequencing batch airlift reactor at low temperature. Journal of Harbin Institute of Technology, 16(4):570–575.

    Google Scholar 

  • Di Iaconi, C., Ramadori, R., Lopez, A., 2006. Combined biological and chemical degradation for treating a mature municipal landfill leachate. Biochemical Engineering Journal, 31(1):118–124. [doi:10.1016/j.bej.2006.06.002]

    Article  Google Scholar 

  • Ford, D.L., Churchwell, R.L., Kachtick, J.W., 1980. Comprehensive analysis of nitrification of chemical processing wastewaters. Journal of the Water Pollution Control Federation, 52(11):2726–2746.

    Google Scholar 

  • Hirooka, K., Asano, R., Nakai, Y., 2009. Change in the community structure of ammonia-oxidizing bacteria in activated sludge during selective incubation for MPN determination. Journal of Industrial Microbiology and Biotechnology, 36(5):679–685. [doi:10.1007/s10295-009-0537-8]

    Article  Google Scholar 

  • LaPara, T.M., Nakatsu, C.H., Pantea, L.M., Alleman, J.E., 2002. Stability of the bacterial communities supported by a severn-stage biological process treating pharmaceutical wastewater as revealed by PCR-DGGE. Water Research, 36(3):638–646. [doi:10.1016/S0043-1354(01)00277-9]

    Article  Google Scholar 

  • Li, A.J., Yang, S.F., Li, X.Y., Gu, J.D., 2008. Microbial population dynamics during aerobic sludge granulation at different organic loading rates. Water Research, 42(13): 3552–3560. [doi:10.1016/j.watres.2008.05.005]

    Article  Google Scholar 

  • Li, D.H., Ganczarczyk, J., 1989. Fractal geometry of particle aggregates generated in water and wastewater treatment processes. Environmental Science and Technology, 23(11):1385–1389. [doi:10.1021/es00069a009]

    Article  Google Scholar 

  • Li, J.T., Ji, S.L., Liu, Z.P., Qin, Z.P., Liu, Y., Yang, Y.Y., 2009. Bacteria composition of aerobic granular sludge analyzed with 16S rDNA clone library. Research of Environmental Sciences, 22(10):1218–1223 (in Chinese).

    Google Scholar 

  • Li, P., Wang, Y.X., Wang, Y.H., Liu, K., Tong, L., 2010. Bacterial community structure and diversity during establishment of an anaerobic bioreactor to treat swine wastewater. Water Science and Technology, 61(1):243–252. [doi:10.2166/wst.2010.807]

    Article  Google Scholar 

  • Li, X.Y., Yuan, Y., 2002. Settling velocities and permeabilities of microbial aggregates. Water Research, 36(12):3110–3120. [doi:10.1016/S0043-1354(01)00541-3]

    Article  Google Scholar 

  • Li, X.Z., Zhao, Q.L., 1999. Inhibition of microbial activity of activated sludge by ammonia in leachate. Environment International, 25(8):961–968. [doi:10.1016/S0160-4120(99)00068-9]

    Article  Google Scholar 

  • Liu, X.C., Zhang, Y., Yang, M., Wang, Z.Y., Lv, W.Z., 2007. Analysis of bacterial community structures in two sewage treatment plants with different sludge properties and treatment performance by nested PCR-DGGE method. Journal of Environmental Sciences, 19(1):60–66. [doi:10.1016/S1001-0742(07)60010-2]

    Article  Google Scholar 

  • Moreno, Y., Botella, S., Alonso, J.L., Ferrus, M.A., Hernandez, M., Hernandez, J., 2003. Specific detection of Arcobacter and Campylobacter strains in water and sewage by PCR and fluorescent in situ hybridization. Applied and Environmental Microbiology, 69(2):1181–1186. [doi:10.1128/AEM.69.2.1181-1186.2003]

    Article  Google Scholar 

  • Muyzer, G., Waal, E.C., Uitterlinden, A.G., 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction amplified genes encoding for 16S rRNA. Applied and Environmental Microbiology, 59(3):695–700.

    Google Scholar 

  • Ni, B.J., Yu, H.Q., 2010. Mathematical modeling of aerobic granular sludge: a review. Biotechnology Advance, 28(6): 895–909. [doi:10.1016/j.biotechadv.2010.08.004]

    Article  Google Scholar 

  • Osaka, T., Yoshie, S., Tsuneda, S., Hirata, A., Iwami, N., Inamori, Y., 2006. Identification of acetate- or methanol-assimilating bacteria under nitrate-reducing conditions by stable-isotope probing. Microbial Ecology, 52(8):253–266. [doi:10.1007/s00248-006-9071-7]

    Article  Google Scholar 

  • Parsley, L.C., Consuegra, E.J., Thomas, S.J., Bhavsar, J., Land, A.M., Bhuiyan, N.N., Mazher, M.A., Waters, R.J., Wommack, K.E., Harper, W.F.Jr., et al., 2010. Census of the viral metagenome within an activated sludge microbial assemblage. Applied and Environmental Microbiology, 76(8):2673–2677. [doi:10.1128/AEM.02520-09]

    Article  Google Scholar 

  • Sanguinetti, C.J., Dias, N.E., Simpson, A.J., 1994. Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. Biotechniques, 17(5):914–921.

    Google Scholar 

  • Su, K.Z., Yu, H.Q., 2005. Formation and characterization of aerobic granules in a sequencing batch reactor treating soybean-processing wastewater. Environmental Science and Technology, 39(8):2818–2828. [doi:10.1021/es048950y]

    Article  Google Scholar 

  • Tarlera, S., Jangid, K., Ivester, A.H., Whitman, W.B. Williams, M.A., 2008. Microbial community succession and bacterial diversity in soils during 77 000 years of ecosystem development. FEMS Microbiology Ecology, 64(1):129–140. [doi:10.1111/j.1574-6941.2008.00444.x]

    Article  Google Scholar 

  • Wang, J.F., 2006. Nitrogen and Phosphorous Removal of Aerobic Granules and Granules Membrane Bioreactor. PhD Thesis, Tianjin University, Tianjin, China (in Chinese).

    Google Scholar 

  • Wang, S.G., Liu, X.W., Gong, W.X., Gao, B.Y., Zhang, D.H., Yu, H.Q., 2007. Aerobic granulation with brewery wastewater in a sequencing batch reactor. Bioresource Technology, 98(11):2142–2147. [doi:10.1016/j.biortech.2006.08.018]

    Article  Google Scholar 

  • Xiao, F., Yang, S.F., Li, X.Y., 2008. Physical and hydrodynamic properties of aerobic granules produced in sequencing batch reactors. Separation and Purification Technology, 63(3):634–641. [doi:10.1016/j.seppur.2008.07.002]

    Article  Google Scholar 

  • Zhang, B., Sun, B.S., Ji, M., Zhao, Z.G., 2008. Analysis and succession of microbial community structure in a membrane bioreactor. Acta Scientiae Circumstantiae, 28(11): 2192–2199 (in Chinese).

    Google Scholar 

  • Zhang, B., Sun, B.S., Ji, M., Liu, H.N., Liu, X.H., 2010. Quantification and comparison of ammonia-oxidizing bacterial communities in MBRs treating various types of wastewater. Bioresource Technology, 101(9):3054–3059. [doi:10.1016/j.biortech.2009.12.048]

    Article  Google Scholar 

  • Zhang, J.J., Li, X.Y., Oh, S.E., Logan, B.E., 2004. Physical and hydrodynamic properties of flocs produced during biological hydrogen production. Biotechnology and Bioengineering, 88(7):854–860. [doi:10.1002/bit.20297]

    Article  Google Scholar 

  • Zhang, L.L., Chen, J.M., Fang, F., 2008. Biodegradation of methyl t-butyl ether by aerobic granules under a cosubstrate condition. Applied Microbiology and Biotechnology, 78(3):543–550. [doi:10.1007/s00253-007-1321-1]

    Article  Google Scholar 

  • Zhuang, W.Q., Tay, J.H., Yi, S., Tay, S.T., 2005. Microbial adaptation to biodegradation of tert-butyl alcohol in a sequencing batch reactor. Journal of Biotechnology, 118(1):45–53. [doi:10.1016/j.jbiotec.2005.02.014]

    Article  Google Scholar 

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Authors and Affiliations

  1. Key Laboratory of Environmental Protection in Water Transport Engineering Ministry of Communications, Tianjin Research Institute of Water Transport Engineering, Tianjin, 300456, China

    Yan-jie Wei & Guo-yi Li

  2. School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, China

    Yan-jie Wei & Min Ji

  3. Tianjin Tanggu Sino French Water Supply Co., Ltd., Tianjin, 300450, China

    Fei-fei Qin

Authors
  1. Yan-jie Wei
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  2. Min Ji
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  3. Guo-yi Li
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  4. Fei-fei Qin
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Correspondence to Min Ji.

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Project supported by the National Water Pollution Control and Management Project of China (No. 2009ZX07314-002), the Tianjin Science and Technology Development Program (No. 06YFGZSH06700), and the Science and Technology Planning Project of Tianjin Binhai New Area, China (No. 2010-Bk130067)

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Wei, Yj., Ji, M., Li, Gy. et al. Microbial and hydrodynamic properties of aerobic granules in a sequencing batch reactor treating landfill leachate. J. Zhejiang Univ. Sci. A 13, 219–229 (2012). https://doi.org/10.1631/jzus.A1100153

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  • DOI: https://doi.org/10.1631/jzus.A1100153

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