Abstract
A mature appressorium cDNA library of rice blast fungus, Magnaporthe grisea was constructed in a λ TriplEx2 vector by SMART™ cDNA library containing 2.37×106 independent clones about 100% of which harbor foreign cDNA inserts with average size of 660 bp. Of 9 randomly selected clones, 2 expressed sequence tags (ESTs) sequences did not have homologous EST sequences of M. grisea in GenBank. The appressorium cDNA library is suitable for gene expression analysis and function analysis of the late stages of appressorium formation and the early stages of penetration of M. grisea.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W., Lipman, D.J., 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.Nucleic Acids Res.,25:3389–3402.
Balhadère, P.V., Foster, A.J., Talbot, N.J., 1999. Identification of pathogenicity mutants of the rice blast fungusMagnaporthe grisea using insertional mutagenesis.Mol. Plant-Microbe Interact.,12:129–142.
Banno, S., Kimura, M., Tokai, T., Kasahara, S., Higa-Nishiyama, A., Takahashi-Ando, N., Hamamoto, H., Fujimura, M., Staskawicz, B.J., Yamaguchi, I., 2003. Cloning and characterization of genes specifically expressed during infection stages in the rice blast fungus.FEMS Microbiol Lett,222:221–227.
Choi, W., Dean, R.A., 1997. The adenylate cyclase geneMAC1 ofMagnaporthe grisea controls appressorium formation and other aspects of growth and development.Plant Cell,9:1973–1983.
Ebbole, D.L., Yuan, J., Li, D., Thomas, T.L., Dean, R.A., 2002. Gene Discovery in the Rice Blast Fungus,Magnaporthe Grisea: Analysis of ESTs.In: Abstracts for Plant. Animal & Microbe Genomes X, San Diego, CA, p. 18.
Hall, T.A., 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT.Nucl. Acids. Symp. Ser.,41:95–98.
Irie, T., Matsumura, H., Terauchi, R., Saitoh, H., 2003. Serial Analysis of Gene Expression (SAGE) ofMagnaporthe grisea: genes involved in appressorium formation.Mol. Genet. Genomics,270:181–189.
Kamakura, T., Xiao, J., Choi, W., Kochi, T., Yamaguchi, S., Teraoka, T., Yamaguchi, I., 1999. cDNA subtractive cloning of genes expressed during early stage of appressorium formation byMagnaporthe grisea.Biosci. Biotechnol. Biochem.,63:1407–413.
Kim, S., Ahn, I.P., Lee, Y.H., 2001. Analysis of genes expressed during rice-Magnaporthe grisea interactions.Mol. Plant-Microbe Interact.,14:1340–346.
Lau, G.W., Hamer, J.E., 1996. Regulatory genes controllingMPG1 expression and pathogenicity in the rice blast fungusMagnaporthe grised.Plant Cell,8:771–781.
Liu, S., Dean, R.A., 1997. G protein α-subunit genes control growth, development and pathogenicity ofMagnaporthe grisea.Mol Plant-Microbe Interact,10:1075–1086.
Rauyaree, R., Choi, W., Fang, E., Blackmon, B., Dean, R.A., 2001. Genes expressed during early stages of rice infection with the rice blast fungusMagnaporthe grisea.Mol. Plant Pathol.,2:347–354.
Shi, Z., Christian, D., Leung, H., 1995. Enhanced transformation inMagnaporthe grisea by restriction enzyme mediated integration of plasmid DNA.Phytopathology,85:329–333.
CLI (CLONTECH Laboratories, Inc.), 2001. Smart cDNA Library Construction Kit User Manual. USA, p. 1–51.
Sweigard, J.A., Carroll, A.M., Farrall, L., Chumley, F.G., Valent, B., 1998.Magnaporthe grisea pathogenicity genes obtained through insertional mutagenesis.Mol. Plant-Microbe Interact.,11:404–412.
Takano, Y., Choi, W., Mitchell, T.K., Okuno, T., Dean, R.A., 2003. Large scale parallel analysis of gene expression during infection-related morphogenesis ofMagnaporthe grisea.Mol. Plant Pathol.,4:337–347.
Talbot, N.J., Ebbole, D.J., Hamer, J.E., 1993. Identification and characterization ofMPG1, a gene involved in pathogenicity from the rice blast fungusMagnaporthe grisea.Plant Cell,5:1575–1590.
Talbot, N.J., Kershaw, M.J., Wakley, G.E., De Vries, O.M.H., Wessels, J.G.H., Hamer, J.E., 1996.MPG1 encodes a fungal hydrophobin involved in surface interactions during infection-related development ofMagnaporthe grisea.Plant Cell,8:985–999.
Viaud, M.C., Balhadere, P.V., Talbot, N.J., 2002. AMagnaporthe grisea cyclophilin acts as a virulence determinant during plant infection.Plant Cell 14:917–930.
Xu, J.R., Hamer, J.E., 1996. MAP kinase and cAMP signaling regulate infection structure formation and pathogenic growth in the rice blast fungusMagnaporthe grisea.Genes Dev.,10:2696–2706.
Xu, J.R., Xue, C.Y., 2002. Time for a blast: genomics ofMagnaporthe grisea.Mol. Plant Pathol.,3:173–176.
Xue, C.Y., Park, G., Choi, W., Zheng, L., Dean, R.A., Xu, J.R., 2002. Two novel fungal virulence genes specifically expressed in appressoria of the rice blast fungus.Plant Cell,14:2107–2119.
Author information
Authors and Affiliations
Corresponding author
Additional information
Project supported by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, and in part by the National Natural Science Foundation of China (No. 30270049)
Rights and permissions
About this article
Cite this article
Lu, Jp., Liu, Tb., Yu, Xy. et al. Representative appressorium stage cDNA library of Magnaporthe grisea. J Zheijang Univ Sci B 6, 132–136 (2005). https://doi.org/10.1631/jzus.2005.B0132
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1631/jzus.2005.B0132