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cDNA library construction from meristematic tissue of finger millet panicle

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Abstract

A protocol to obtain full-length cDNA using a SuperScript® Full-Length cDNA Library Construction Kit II (Invitrogen, United States) was developed, and a high quality cDNA library of meristem tissue of finger millet panicle (Eleusine coracana (L.) Gaertn) was constructed. The titer of the constructed cDNA library was 3.01 × 105 CFU/mL, the average length of the insert was approximately 1070 base pairs, and the average efficiency of insertion of cDNA fragments was 99.5%. The sequencing of randomly selected clones created cDNA library was carried out. The cDNA sequences of clones were identified by BLAST search. The cDNA library analysis and selective sequencing indicate good functionality and full size of cDNA inserts of the clones. The constructed cDNA library from meristematic tissue of finger millet panicle is a good and reliable source for isolation and identification of key genes of metabolism and development of meristem as well for creation of new genetic markers for genetic research and molecular selection.

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Authors and Affiliations

  1. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, Gatersleben, 06466, Germany

    V. Radchuk

  2. Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, ul. Osipovskogo 2A, Kiev, 04123, Ukraine

    Ya. V. Pirko, S. V. Isayenkov, A. I. Yemets & Ya. B. Blume

Authors
  1. V. Radchuk
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  2. Ya. V. Pirko
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  3. S. V. Isayenkov
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  4. A. I. Yemets
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  5. Ya. B. Blume
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Corresponding author

Correspondence to V. Radchuk.

Additional information

Original Russian Text © V. Radchuk, Ya.V. Pirko, S.V. Isayenkov, A.I. Yemets, Ya.B. Blume, 2014, published in Tsitologiya i Genetika, 2014, Vol. 48, No. 5, pp. 3–10.

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Radchuk, V., Pirko, Y.V., Isayenkov, S.V. et al. cDNA library construction from meristematic tissue of finger millet panicle. Cytol. Genet. 48, 273–278 (2014). https://doi.org/10.3103/S0095452714050089

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  • DOI: https://doi.org/10.3103/S0095452714050089

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