Abstract
An increasing number of monopartite begomoviruses are being identified that a satellite molecule (DNAβ) is required to induce typical symptoms in host plants. DNAβ encodes a single gene (termed βC1) encoded in the complementary-sense. We have produced transgeni cNicotiana benthamiana and N. tabacum plants expressing the βC1 gene of a DNAβ associated with plants expressing βC1 showed severe developmental abnormalities in both species. Microscopic analysis of sections of both transgenic and non-transgenic N. tabacum leaves showed abnormal outgrowths of transgenic N. tabacum to be due to disorganized cell division (hyperplasia) of spongy and palisade parenchyma. Immuno-gold labeling of sections with a polyclonal antibody against the βC1 protein showed that the βC1 protein accumulated in the nuclei of cells. The possible biological function of the βC1 protein was discussed.
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Projects (Nos. 30270072 and 30125032) supported by the National Natural Science Foundation of China
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Cui, Xf., Li, Yq., Hu, Dw. et al. Expression of a begomoviral DNAβ gene in transgenic Nicotiana plants induced abnormal cell division. J Zheijang Univ Sci B 6, 83–86 (2005). https://doi.org/10.1631/jzus.2005.B0083
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DOI: https://doi.org/10.1631/jzus.2005.B0083