Abstract
Plastids of nongreen tissues import carbon as a source of biosynthetic pathways and energy, and glucose 6-phosphate is the preferred hexose phosphate taken up by nongreen plastids. A cDNA clone encoding glucose 6-phosphate/phosphate translocator (GPT) was isolated from a cDNA library of immature seeds of rice and named asOsGPT. The cDNA has one uninterrupted open reading frame encoding a 42 kDa polypeptide possessing transit peptide consisting of 70 amino acid residues. TheOsGPT gene maps on chromosome 8 of rice and is linked to the quantitative trait locus for 1000-grain weight. The expression ofOsGPT is mainly restricted to heterotrophic tissues. These results suggest that glucose 6-phosphate imported viaGPT can be used for starch biosynthesis in rice nongreen plastids.
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Borchert, S., Harborth, J., Schünemann, D., Hoferichter, P. and Heldt, H.W., 1993. Studies of the enzymatic capacities and trans-port properties of pea root plastids.Plant Physiol.,101: 303–312.
Denyer, K., Dunlap, F., Thorbjornsen, T., Keeling, P. and Smith, A. M., 1996. The major form of ADP-glucose pyrophosphorylase in maize endosperm is extraplastidial.Plant Physiol.,112: 779–785.
Harrison, C. J., Mould, R. M., Leech, M. J., Johnson, S. A., Turner, L., Schreck, S. L., Baird, K. M., Jack, P. L., Rawsthorne, S., Hedley, C. L. and Wang, T. L., 2000. The rug3 locus of pea encodes plastidial phosphoglucomutase.Plant Physiol.,122: 1187–1192.
Hill, L. M. and Smith, A. M., 1991. Evidence that glucose 6-phosphate is imported as the substrate for starch biosynthesis by the plastids of developing pea embryos.Planta,185: 91–96.
Kammerer, B., Fischer, K., Hilpert, B., Schubert, S., Gutensohn, M., Weber, A. and Flugge, U. I., 1998. Molecular characterization of a carbon transporter in plastids from heterotrophic tissues: the glucose 6-phosphate/phosphate antiporter.Plant Cell,10: 105–117.
Kofler, H., Hausler, R. E., Schulz, B., Groner, F., Flugge, U. I. and Weber, A., 2000. Molecular characterisation of a new mutant allele of the plastid phosphoglucomutase in Arabidopsis, and complementation of the mutant with the wild-type cDNA.Mol. Gen. Genet.,263: 978–986.
Lu, C., Shen, L., Tan, Z., Xu, Y., He, P., Chen, Y. and Zhu, L., 1996 Comparative mapping of QTLs for agronomic traits of rice across environments using a doubled haploid population.Theor. Appl. Genet.,93: 1211–1217.
Naeem, M., Tetlow, I. J. and Emes, M. J., 1997. Starch synthesis in amyloplasts purified from developing potato tubes.Plant J.,11: 1095–1103.
Neuhaus, H. E., Thom, E., Batz, O. and Scheibe, R., 1993. Purification of highly intact plastids from various heterotrophic plant tissues. Analysis of enzyme equipment and precursor dependency for starch biosynthesis.Biochem. J.,296: 395–401.
Neuhaus, H. E. and Maas, U., 1996. Unidirectional transport of orthophosphate across the envelope of isolated cauli-flower bud amyloplasts.Planta,198: 542–548.
Sikka, V. K., Choi, S. B., Kavakli, I. H., Sakulsingharoj, C., Gupta, S., Ito, H. and Okita, T. W., 2001. Subcellular compartmentation and allosteric regulation of the rice endosperm ADPglucose pyrophosphorylase.Plant Sci.,161: 461–468.
Sullivan, T. D. and Kaneko, Y., 1995. The maize brittlel gene encodes amyloplast membrane polypeptides.Planta,196: 477–484.
Tauberger, E., Fernie, A. R., Emmermann, M., Renz, A., Kossmann, J., Willmitzer, L. and Trethewey, R. N., 2000. Antisense inhibition of plastidial phosphoglucomutase provides compelling evidence that potato tuber amyloplasts import carbon from the cytosol in the form of glucose-6-phosphate.Plant J.,23: 43–53.
Tetlow, J., Blisset, K. J. and Emes, M. J., 1994. Starch synthesis and carbohydrate oxidation in amyloplasts from developing wheat endosperm.Planta,194: 454–460.
Thorbjornsen, T., Villand, P., Denyer, K., Olsen, O. A. and Smith, A. M., 1996. Distinct isoforms of ADPglucose pyrophosphorylase occur inside and outside the amyloplasts in barley endosperm.Plant J.,10: 243–250.
Wu, J., Maehara, T., Shimokawa, T., Yamamoto, S., Harada, C., Takazaki, Y., Ono, N., Muka, I. Y., Koike, K., Yazaki, J., Fujii, F., Shomura, A., Ando, T., Kono, I., Waki, K., Yamamoto, K., Yano, M., Matsumoto, T. and Sasaki, T., 2002. A comprehensive rice transcript map containing 6591 expressed sequence tag sites.Plant Cell,14: 525–535.
Xiao, J., Li, J., Yuan, J. and Tanksley, S. D., 1995. Dominance is the major genetic basis of heterosis in rice as revealed by QTL analysis using molecular markers.Genetics,140: 745–754.
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Project supported by National Natural Scienc Foundation of China (No.39830250) and Natural Science Foundation of Zhejiang Province (No.2A0106), China. The nucleotide sequence data will appear in the GenBank under accession number AF375053.
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Jiang, Hw., Dian, Wm., Liu, Fy. et al. Cloning and characterization of a glucose 6-phosphate/phosphate translocator fromOryza sativa. J. Zhejiang Univ. Sci. A 4, 331–335 (2003). https://doi.org/10.1631/jzus.2003.0331
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DOI: https://doi.org/10.1631/jzus.2003.0331