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Tri-metallic AM-Ag/CuO/ZnO NC synthesized via green chemistry principle and its biomedical applications

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Abstract

Complex tri-metallic AM-Ag/CuO/ZnO NC (NC = Nano-composite) was derived by applying green chemistry principles and optimized via different parameters using simple optical properties of the constituent metals. Primary optimization of AM-Ag/CuO/ZnO NC was done by UV–VIS-NIR spectrophotometer, FTIR spectral report with respect to A. monophylla leaf extract, demonstrated specific peaks in the range 802 to 466 cm−1 which validated the collective synthesis of nano-composite. XRD analysis evaluated the dominant face-centred cubic (FCC) crystal phase over monoclinic and hexagonal phases with an average diameter of 10.20 nm. The morphology, shape, crystalline nature and average diameter were characterized by HR-SEM, HR-TEM images, SAED pattern and micrograph images respectively. Tri-metallic AM-Ag/CuO/ZnO NC has shown an admirable zone of inhibition against eight pathogenic bacteria and potential in-vitro antioxidant activity against DPPH and ABTS free radicals. Nano-composite also well-established the selective inhibition of cervical cancer cell lines compared to (immortal) human embryonic kidney cell lines.

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Figure 1
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Acknowledgments

The authors would like to express gratitude to Pondicherry University to the Department of Ecology & Environmental Sciences for the lab facility, to the Department of Biotechnology for providing the lab facility for carrying out antibacterial and anticancer activity tests and to Central Instrumentation Facility (CIF) for the characterization of the synthesized material. The authors would also like to express thanks to SAIF IIT Madras for the HR-SEM characterization and Thermal analysis of the material.

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Authors and Affiliations

  1. Department of Ecology & Environmental Sciences, Pondicherry University, R. V. Nagar, Puducherry, 605014, India

    Ravikant Verma, Anisa Basheer Khan, Arvind Kumar & Agam Kumar

  2. Department of Biotechnology, Pondicherry University, R. V. Nagar, Puducherry, 605014, India

    Ashutosh Kumar Amar, Mohd. Imran K. Khan & Suresh Sah

  3. Department of Nanoscience and Technology, Central University of Jharkhand, Ranchi, Jharkhand, 835222, India

    Ram Kishore Singh

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  1. Ravikant Verma
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Contributions

Project concept design and laboratory work especially related to material synthesis, characterization and applications was performed by RV. In addition to project concept, critical assessment and editing, manuscript uploading was done by ABK. An antibacterial test and data analysis were performed under the continuous observation of AKA. MIKK’s contribution was in his keen observation throughout the in-vitro experiment for anticancer activity and further helped in anticancer data analysis. AK helped in collection of plant materials, sample processing and material characterization. AK sketched the antibacterial activity graph and done the synergistic and antagonistic study via GraphPad prism software. SS provided critical assessment during MIC and MBC study. RS contributed in characterizing Trimetallic AM-Ag/CuO/ZnO NC, especially for XRD pattern and thermal analysis. His contribution during second revision was partly also in designing Scheme 1.

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Correspondence to Anisa Basheer Khan.

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Verma, R., Khan, A.B., Amar, A.K. et al. Tri-metallic AM-Ag/CuO/ZnO NC synthesized via green chemistry principle and its biomedical applications. Journal of Materials Research 38, 4641–4654 (2023). https://doi.org/10.1557/s43578-023-01184-6

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