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Sequential immobilization of sirolimus and polyethylene glycol on ultrafine-grained commercial pure titanium for cardiovascular stent application

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Abstract

The present research focuses on the development of ultrafine-grained commercial pure titanium (cpTi)/sirolimus/polyethylene glycol (PEG)-coated substrate via a layer-by-layer process. Alkali treatment is performed on processed cpTi (repetitive corrugation and straightening technique) to get a submicron porous structure holding hydroxyl groups for immobilizing sirolimus and PEG coating. The successful sirolimus loading and PEG coating are confirmed through physicochemical characterizations. The sirolimus release pattern without and with PEG coating in PBS for 55 days follows diffusion and case II transport through the Ritger–Peppas model. The antithrombotic performance showed that PEG-coated substrate has higher absorbance (0.572 ± 0.026), i.e., fewer RBCs attached to its surface. The cell viability and adhesion for substrates are determined with interstitial cells. An optimal sirolimus concentration (300 µg/mL) is obtained by sirolimus toxicity assay in the interstitial cell line. Therefore, the ultrafine-grained cpTi-based sirolimus-eluting substrate can be a promising candidate for cardiovascular stent application.

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The raw/processed data required to reproduce these findings cannot be shared at this time as the data also forms part of an on-going study.

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Acknowledgments

The authors thank Mishra Dhatu Nigam Limited, Hyderabad, India, for supplying titanium material and Dr. Devendra Verma, Tissue Engineering and Biomaterials Laboratory, Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, for providing cell culture research facilities.

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No funding was received for conducting this study.

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Authors and Affiliations

  1. Department of Biotechnology and Medical Engineering, National Institute of Technology Rourkela, Odisha, 769008, India

    Monalisha Mohanta & A. Thirugnanam

  2. Department of Biotechnology and Medical Engineering, National Institute of Technology Rourkela, Room No. 206, Odisha, 769008, India

    A. Thirugnanam

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  1. Monalisha Mohanta
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Correspondence to A. Thirugnanam.

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Mohanta, M., Thirugnanam, A. Sequential immobilization of sirolimus and polyethylene glycol on ultrafine-grained commercial pure titanium for cardiovascular stent application. Journal of Materials Research 37, 3559–3574 (2022). https://doi.org/10.1557/s43578-022-00724-w

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