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Electrochemical biosensor utilizing dual-mode output for detection of lung cancer biomarker based on reduced graphene oxide-modified reduced-molybdenum disulfide multi-layered nanosheets

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Abstract

Here, we present an electrochemical biosensor for sensitive, specific, and rapid detection of lung cancer biomarker, neuron-specific enolase by utilization of rGO-modified r-MoS2 multi-layered nanosheets as a matrix. Layered structures along with the synergetic effect of r-MoS2 and rGO allow easy grafting of two materials into one another. This empowers the sensor to have a heterogeneous rate transfer constant of 1.29 × 10–3 cm/s and an electroactive surface area of 27.83 mm2. Electrochemical impedance spectroscopy and cyclic voltammetry are used as dual modes for the detection of NSE, and the sensor exhibits a wide linear detection range. The sensor is found to be reproducible with a relative standard deviation of less than 5% along with an invariant response towards other endogenous interfering species found in human serum. The investigations done in this study will further help us to fabricate a device for rapid and early detection of lung cancer.

Graphical abstract

Drop casting of lung cancer biomarker (NSE) onto BSA/anti-NSE/r-MoS2-rGO/ITO bioelectrode and its electrochemical response using EIS and CV.

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Acknowledgments

Authors are highly obliged to Hon’ble Vice-Chancellor, Delhi Technological University, Delhi, India for promoting research and providing appropriate infrastructure and facilities.

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  1. Nanomaterials Research Laboratory, Department of Applied Physics, Delhi Technological University, Delhi, 110042, India

    Ritika Khatri & Nitin K. Puri

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  1. Ritika Khatri
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Khatri, R., Puri, N.K. Electrochemical biosensor utilizing dual-mode output for detection of lung cancer biomarker based on reduced graphene oxide-modified reduced-molybdenum disulfide multi-layered nanosheets. Journal of Materials Research 37, 1451–1463 (2022). https://doi.org/10.1557/s43578-022-00546-w

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