Skip to main content
SpringerLink
Log in

Comparison of Demineralized and Deproteinized Bone

  • Biomineralization and Bioinspired Inorganic and Inorganic/Organic Materials
  • Published:
MRS Online Proceedings Library Aims and scope

Abstract

Cortical and cancellous bones were demineralized and deproteinized using 1 N HCl and 6% NaOCl, respectively. Experiments were performed at 37°C. The rate constants were calculated and the structural features of untreated and completely demineralized and deproteinized samples were studied by scanning electron microscopy, showing that intact, contiguous structures were obtained. For both cases, the rate constant was higher for cancellous bone than the cortical bone.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. D. A. Chakkalakal, B. S. Strates, K. L. Garvin, J. R. Novak, E. D. Fritz, T. J. Moliner and M. H. McGuire. Tissue Eng. 7, 161 (2001).

    Article  CAS  Google Scholar 

  2. J. R. Mauney, C. Jaquiéry, V. Volloch, M. Heberer, I. Martin and D. L. Kaplan. Biomater. 26, 3173 (2005).

    Article  CAS  Google Scholar 

  3. X. Li, L. Jin, G. Balian, C. T. Laurencin and D. G. Anderson. Biomater. 27, 2426 (2006).

    Article  CAS  Google Scholar 

  4. Z.–H. Wang, X-J He, Z-Q Yang and J-B Tu. Artif. Organs. 34, 161 (2010).

    Article  CAS  Google Scholar 

  5. R.A. Dodds, A.M. York-Ely, R. Zhukauskas, T. Arola, J. Howell, C. Hartill, R.R. Cobb and C. Fox. J. Biomater. Appl. 25, 195 (2010).

    Article  CAS  Google Scholar 

  6. D. Benke, A. Olah and H. Möhler. Biomaterials. 22, 1005 (2001).

    Article  CAS  Google Scholar 

  7. D. H. Carter, A. J. Scully, D. A. Heaton, M. P. J. Young, and J. E. Aaron. Bone. 31, 389 (2002).

    Article  CAS  Google Scholar 

  8. A. Stavropoulos and T. Karring. J. Clin. Periodontol. 37, 200 (2010).

    Article  Google Scholar 

  9. R. Lakes. Nature 361, 511 (1993).

    Article  Google Scholar 

  10. J-Y Rho, L. Kuhn-Spearing and P. Zioupos. Med. Eng. Phys. 20, 92 (1998).

    Article  CAS  Google Scholar 

  11. S. Weiner and H. D. Wagner. Annu. Rev. Mater. Sci. 28, 271 (1998).

    Article  CAS  Google Scholar 

  12. P.-Y. Chen, A. Y. M. Lin, Y.-S. Lin, Y. Seki, A.G. Stokes, J. Peyras, E. A. Olevsky, M.A. Meyers and J. McKittrick. J. Mech. Behav. Biomed. Mater. I, 208 (2008).

    Article  Google Scholar 

  13. H. Birkedal-Hansen. J. Histochem. Cytochem. 22, 428 (1974).

    Article  CAS  Google Scholar 

  14. H. Birkedal-Hansen. J. Histochem. Cytochem. 22, 434 (1974).

    Article  CAS  Google Scholar 

  15. A.B. Castro-Ceseña, E.E. Novitskaya, P.-Y. Chen, G.A. Hirata, and J. McKittrick. Mat. Sci. Eng. C. (2010) doi: 10.1016/j.msec.2010.11.003.

  16. J. D. Termine, E. D. Eanes, D. J. Greenfield, M. U. Nylen and R. A. Harbor. Calc. Tiss. Res. 12, 73 (1973).

    Article  CAS  Google Scholar 

  17. J. J. Broz, S. J. Simske, W. D. Corley and A. R. Greenberg. J. Mater. Sci. - Mater. Med. 8, 395 (1997).

    Article  CAS  Google Scholar 

  18. G. E. Fantner, T. Hassenkam, J. H. Kindt, J. C. Weaver, H. Birkedal, L. Pechenik, J. A. Cutroni, G. A. Cidade, G. D. Stuky, D. E. Morse and P. K. Hansma. Nat. Mater. 4, 612 (2005).

    Article  CAS  Google Scholar 

  19. P. J. Thurner, B. Erickson, P. Turner, R. Jungmann, J. Lelujian, A. Proctor, J. C. Weaver, G. Schitter, D. E. Morse and P. K. Hansma. Adv. Mater. 21, 451 (2009).

    Article  CAS  Google Scholar 

  20. O. H. Lowry, N. J. Rosebrough, A. l. Farr and R. J. Randall. J. Biol. Chem. 193, 265 (1951).

    CAS  Google Scholar 

  21. A. Bigi, G. Cojazzi, S. Panzavolta, A. Ripamonti, N. Roveri, M. Romanello, K. Noris Suarez and L. Moro. J. Inorg. Biochem. 68, 45 (1997).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Carretera Tijuana-Ensenada No. 3918, Zona Playitas, 22860, Ensenada, Baja California, México

    Ana B. Castro-Ceseña & M. Pilar del Sánchez-Saavedra

  2. UC San Diego, 9500 Gilman Dr., La Jolla, CA, 92093, USA

    Ekaterina Novitskaya, Po-Yu Chen & Joanna McKittrick

  3. Centro de Nanociencias y Nanotecnología, UNAM (CNyN-UNAM), Km 107 Carretera Tijuana-Ensenada, Apo. Postal, 356, CP. 22800, Ensenada, México

    Gustavo Hirata

Authors
  1. Ana B. Castro-Ceseña
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ekaterina Novitskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Po-Yu Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Pilar del Sánchez-Saavedra
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Gustavo Hirata
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Joanna McKittrick
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Castro-Ceseña, A.B., Novitskaya, E., Chen, PY. et al. Comparison of Demineralized and Deproteinized Bone. MRS Online Proceedings Library 1301, 27–32 (2011). https://doi.org/10.1557/opl.2011.194

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1557/opl.2011.194

Search

Navigation