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PEDOT:PSS microelectrode arrays for hippocampal cell culture electrophysiological recordings

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Abstract

In vitro electrophysiology using microelectrode arrays (MEAs) plays an important role in understanding fundamental biologic processes, screening potential drugs and assessing the toxicity of chemicals. Low electrode impedance and ability to sustain viable cultures are the key technology requirements. We show that MEAs consisting of poly(3,4-ethylenedioxythiophene) doped with poly(styrene sulfonate) (PEDOT:PSS) and coated with poly-L-lysine satisfy these requirements. Hippocampal cell cultures, maintained for 3–6 weeks on these MEAs, give high quality recordings of neural activity. This enables the observation of drug-induced activity changes, which paves the way for using these devices in in vitro drug screening and toxicology applications.

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Acknowledgements

The authors would like to thank the Marie Curie initial training network OLIMPIA for the financial support. The MEAs were fabricated at the Centre Microélectronique de Provence (Gardanne, France) clean room facilities while the cell culturing (B.H. and C.F.) and the electrophysiology (E.D.) measurements were performed at MyEnterix laboratories in Aix-Marseille University, CNRS, CRN2M, Marseille, France. The authors declare no competing financial interest.

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Authors and Affiliations

  1. Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, 13541, Gardanne, France

    Dimitrios A. Koutsouras, Adel Hama, Jolien Pas, Paschalis Gkoupidenis, Róisín M. Owens & George G. Malliaras

  2. CNRS, CRN2M, Aix Marseille University, Marseille, France

    Bruno Hivert, Catherine Faivre-Sarrailh & Eric Di Pasquale

  3. CSO@MyEnterix, 13344, Marseille, France

    Bruno Hivert, Catherine Faivre-Sarrailh & Eric Di Pasquale

Authors
  1. Dimitrios A. Koutsouras
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  2. Adel Hama
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  3. Jolien Pas
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  4. Paschalis Gkoupidenis
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  5. Bruno Hivert
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  6. Catherine Faivre-Sarrailh
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  7. Eric Di Pasquale
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  8. Róisín M. Owens
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  9. George G. Malliaras
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Corresponding author

Correspondence to George G. Malliaras.

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This author was an editor of this journal during the review and decision stage. For the MRC policy on review and publication of manuscripts authored by editors, please refer to http://www.mrs.org/editor-manuscripts/.

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The supplementary material for this article can be found at https://doi.org/10.1557/mrc.2017.34

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Koutsouras, D.A., Hama, A., Pas, J. et al. PEDOT:PSS microelectrode arrays for hippocampal cell culture electrophysiological recordings. MRS Communications 7, 259–265 (2017). https://doi.org/10.1557/mrc.2017.34

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  • DOI: https://doi.org/10.1557/mrc.2017.34

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