Abstract
In this study, we present a 4-channel intracortical glassy carbon (GC) microelectrode array on a flexible substrate for the simultaneous in vivo neural activity recording and dopamine (DA) concentration measurement at four different brain locations (220μm vertical spacing). The ability of GC microelectrodes to detect DA was firstly assessed in vitro in phosphate-buffered saline solution and then validated in vivo measuring spontaneous DA concentration in the Striatum of European Starling songbird through fast scan cyclic voltammetry(FSCV). The capability of GC microelectrode arrays and commercial penetrating metal microelectrode arrays to record neural activity from the Caudomedial Neostriatum of European starling songbird was compared. Preliminary results demonstrated the ability of GC microelectrodes in detecting neurotransmitters release and recording neural activity in vivo. GC microelectrodes array may, therefore, offer a new opportunity to understand the intimate relations linking electrophysiological parameters with neurotransmitters release.
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References
M. van Spronsen and C. C. Hoogenraad, Curr Neurol Neurosci Rep. 10(3), 207 (2010).
R. Brisch, A. Saniotis, R. Wolf, H. Bielau, H. G. Bernstein, J. Steiner, B. Bogerts, K. Braun, Z. Jankowski, J. Kumaratilake, M. Henneberg, T. Gos, Front Psychiatry 5, 47 (2014).
J. Lotharius and P. Brundin, Nature Reviews Neuroscience 3(12), 932 (2002).
N. D. Volkow, J.S.Fowler, G. J. Wang, Behav Pharmacol. 13(5–6), 355 (2002).
E. Castagnola, S. Carli, M. Vomero, A. Scarpellini, M. Prato, N. Goshi, L. Fadiga, S. Kassegne, D. Ricci, Biointerphases 12(3), 031002 (2017).
M. L. Mundroff and M. R. Wightman, Curr Protoc Neurosci Chapter 6: Unit 6.14, (2002).
P. S. Cahill, Q. D. Walker, J. M. Finnegan, G. E. Mickelson, E. R. Travis, M. R. Wightman, Anal Chem 68, 3180 (1996).
I. M. Taylor, E. M. Robbins, K. A. Catt, P. A. Cody, C. L. Happe, X.T. Cui, Biosens Bioelectron 89(Pt 1), 400 (2017).
N. Xiao and B. J. Venton, Anal Chem. 84(18), 7816 (2012).
W.Yi, Y. Yang, P. Hashemi, M. Ming-Cheng Cheng, Biomed Microdevices 18, 112 (2016).
M. K. Zachek, P. Takmakov, B. Moody, R. M. Wightman, G. S. McCarty, Anal Chem 81(15), 6258 (2009).
M. Vomero, E. Castagnola, F. Ciarpella, E. Maggiolini, N. Goshi, E. Zucchini, S. Carli, L. Fadiga, S. Kassegne, D. Ricci, Scientific Reports 7, 40332 (2016).
M.Vomero, P.van Niekerk, V. Nguyen, N.Gong, M. Hirabayashi, A. Cinopri, K. Logan, A. Moghadasi, P.Varma and S. Kassegne, J Micromechanic Microengineer 26, 2 (2016).
K. Simonyan, B. Horwitz, and E. D. Jarvis, Brain Lang 122(3), 142 (2012).
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Castagnola, E., Vahidi, N.W., Nimbalkar, S. et al. In Vivo Dopamine Detection and Single Unit Recordings Using Intracortical Glassy Carbon Microelectrode Arrays. MRS Advances 3, 1629–1634 (2018). https://doi.org/10.1557/adv.2018.98
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DOI: https://doi.org/10.1557/adv.2018.98