Skip to main content
SpringerLink
Log in

Quantification of Saponins in Different Plant Parts of Lysimachia L. Species by validated HPTLC—Densitometric Method

  • Original Research Papers
  • Published:
JPC – Journal of Planar Chromatography – Modern TLC Aims and scope Submit manuscript

Summary

Quantitative high-performance thin-layer chromatography (HPTLC) analysis of triterpene saponins in different parts of eight Lysimachia L. species is described. For separation, silica gel F254 plates were used with chloroform-methanol-water (8:7:1, v/v) as mobile phase 1 and with n-butanol-acetic acid-water (6:1:3, v/v) as mobile phase 2. The method was validated for selectivity, sensitivity, accuracy, precision, and indirect precision. Saponins were most abundant in the underground parts of all analyzed species, where their content ranged from 0.12% in Lysimachia clethroides to 1.18% in Lysimachia ephemerum. The saponin levels in aerial parts of the plant were generally lower, the only exception being L. ephemerum leaf (1.57%) and Lysimachia thyrsiflora stem (2.26%). The method is sensitive with good precision and accuracy and may be suitable for comparison of the differences in saponin content among samples from various related species and genera.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. J.K. Tian, L.Z. Xu, Z.M. Zou, S.L. Yang, Chem. Pharm. Bull. 54 (2006) 567–569.

    Article  CAS  Google Scholar 

  2. M. Challam, B. Roy, V. Tandon, Vet. Parasitol. 169 (2010) 214–218.

    Article  CAS  Google Scholar 

  3. H.Y. Li, Z.B. Hao, X.L. Wang, L. Huang, J.P. Li, Bioresour. Technol. 100 (2009) 970–974.

    Article  CAS  Google Scholar 

  4. M. Källersjö, G. Bergqvist, A. Anderberg, Am. J. Botany 87 (2000) 1325–1341.

    Article  Google Scholar 

  5. G. Hao, Y.M. Yuan, C.M. Hu, X.J. Ge, N.X. Zhao, Mol. Phylogen. Evolution 31 (2004) 323–339.

    Article  CAS  Google Scholar 

  6. J.B. Harborne, Phytochemistry 7 (1968) 1215–1230.

    Article  CAS  Google Scholar 

  7. I. Podolak, M. Strzałka, Chromatographia 67 (2008) 471–475.

    Article  CAS  Google Scholar 

  8. X. Huang, R. Yang, J. Trop. Subtrop. Bot. 15 (2007) 175–182.

    CAS  Google Scholar 

  9. K. Foubert, M. Theunis, S. Apers, A.J. Vlietinck, L. Pieters, Curr. Org. Chem. 12 (2008) 629–642.

    Article  CAS  Google Scholar 

  10. W.A. Oleszek, J. Chromatogr. A 967 (2002) 147–162.

    Article  CAS  Google Scholar 

  11. O.P. Sharma, N. Kumar, B. Singh, T.K. Bhat, Food Chem. 132 (2012) 671–674.

    Article  CAS  Google Scholar 

  12. M.H.B.L. Theunis, K. Foubert, J. Pollier, M. Gonzalez-Guzman, A. Goossens, A.J. Vlietinck, L.A.C. Pieters, S. Apers, Phytochemistry 68 (2007) 2825–2830.

    Article  CAS  Google Scholar 

  13. I. Podolak, Z. Janeczko, A. Galanty, M. Michalik, D. Trojanowska, Acta Pol. Pharm. Drug Res. 64 (2007) 39–43.

    CAS  Google Scholar 

  14. ICH-Q2 (R1) Validation and Analytical Procedures: Text and Methodology, International Conference on Harmonization, Geneva, November 2005, http://www.ich.org

  15. K. Ferenci-Fodor, B. Renger, Z. Végh, J. Planar Chromatogr. 23 (2010) 173–179.

    Article  Google Scholar 

  16. B. Renger, Z. Végh, K. Ferenci-Fodor, J. Chromatogr. A 1218 (2011) 2712–2721.

    Article  CAS  Google Scholar 

  17. B. Spangenberg, K.F. Klein, J. Mannhardt, J. Planar Chromatogr. 15 (2002) 204–209.

    Article  CAS  Google Scholar 

  18. D.H. Shewiyo, E. Kaale, P.G. Risha, B. Dejaegher, J. Smeyers-Verbeke, J. Pharm. Biomed. Anal. 66 (2012) 11–23.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacognosy, Pharmaceutical Faculty, Medical College, Jagiellonian University, Medyczna 9, 30-688, Cracow, Poland

    Irma Podolak & Zbigniew Janeczko

  2. Department of Inorganic and Analytical Chemistry, Pharmaceutical Faculty, Medical College, Jagiellonian University, Medyczna 9, 30-688, Cracow, Poland

    Urszula Hubicka, Barbara Żuromska-Witek & Jan Krzek

Authors
  1. Irma Podolak
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Urszula Hubicka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Barbara Żuromska-Witek
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zbigniew Janeczko
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jan Krzek
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Irma Podolak.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Podolak, I., Hubicka, U., Żuromska-Witek, B. et al. Quantification of Saponins in Different Plant Parts of Lysimachia L. Species by validated HPTLC—Densitometric Method. JPC-J Planar Chromat 26, 248–253 (2013). https://doi.org/10.1556/JPC.26.2013.3.8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1556/JPC.26.2013.3.8

Key Words

Search

Navigation