Abstract
A liquid chromatography mass spectrometry (LC-MS) method was developed for simultaneous quantitative analysis of Achyranthes root saponins: chikusetsusaponins IVa (1) and V (2), achyranthosides B (3), C (4), D (5), E (6), and G (7), sulfachyranthosides B (8) and D (9), and betavulgarosides II (10) and IV (11). Satisfactory separation of the saponins was achieved with the use of a volatile ion-pair reagent (dihexyl ammonium acetate) on a phenyl-hexylated silica gel column, and the amounts of saponins extracted under three different conditions were determined. When Achyranthes root was extracted with water at room temperature, achyranthosides B (3) and D (5) were the major saponins, and smaller amounts of other saponins (4, 6–11) were present. However, the amounts of chikusetsusaponins (1 and 2) were negligible. Under the condition to make a standard decoction of a Kampo formula, the major saponins were achyranthosides B (3), C (4), and D (5), and small amounts of chikusetsusaponins IVa (1) and V (2) appeared, whereas prolonged heating largely increased the amounts of chikusetsusaponins. This method can be used for quality control of Achyranthes root.
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This work was supported in part by MEXT-Supported Program for the Strategic Research Foundation at Private Universities (S1101003) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
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Kawahara, Y., Hoshino, T., Morimoto, H. et al. LC-MS-based quantification method for Achyranthes root saponins. J Nat Med 70, 102–106 (2016). https://doi.org/10.1007/s11418-015-0942-4
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DOI: https://doi.org/10.1007/s11418-015-0942-4