Summary
A quantitative thin layer chromatographic (TLC) method on silica gel, with ethyl acetate–acetone–alcohol, 62 + 40 + 3 (v/v) as mobile phase, has been developed for the determination of olaquindox at ppm levels in animal feed. Preliminary purification consists of extraction with water, adsorption on XAD-4, washing, and elution with acetonitrile. Before the elution step acetylolaquindox is added as internal standard. The residue obtained after evaporation of acetonitrile is dissolved in a small volume of methanol. Quantitation is performed by densitometry at λ= 360 nm.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
H. Knapstein, Landwirtsch. Forsch. 30 (1977) 94–101.
G.F. Bories, J. Chromatogr. 172 (1979) 505–508.
T. Spierenburg, H. Van Lenthe, G. de Graaf, and L.P. Jager, J. Assoc. Off. Anal. Chem. 71 (1988) 1106–1109.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Roets, E., Quintens, I., Kibaya, R. et al. Quantitative Determination of Olaquindox in Animal Feed. JPC-J Planar Chromat 14, 347–349 (2001). https://doi.org/10.1556/JPC.14.2001.5.9
Published:
Issue Date:
DOI: https://doi.org/10.1556/JPC.14.2001.5.9