Abstract
The glutenin allele gene-pool, the distribution of the individual alleles on the 6 loci coding for glutenin subunits and their combinations were determined in a sample population containing 107 cultivars bred and grown in Martonvásár, Hungary at the Agricultural Research Institute of the Hungarian Academy of Sciences. The database is based on the results of three independent analytical procedures carried out using the traditional SDS-PAGE based allele identification, the state-of-art MALDI-TOF technology and the high throughput capillary electrophoresis based on the lab-on-a-chip technique. The usefulness of integrating the information on both HMW GS and LMW GS allelic composition for future genetic and technological improvement is discussed.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- HMWGS:
-
high molecular weight glutenin subunits
- LMWGS:
-
low molecular weight glutenin subunits
- SDS-PAGE:
-
sodium dodecyl sulphate polyacrylamide gel electrophoresis
- MALDI-TOF-MS:
-
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
- LOC-CE:
-
lab-on-a-chip capillary electrophoresis
References
Appels, R., Gustafson, J.P., O’Brien, L. 2001. Wheat breeding in the new century: Applying molecular genetic analysis of key quality and agronomic traits. Aust. J. Agric. Res. 52:1043–1417.
Balázs, G., Baracskai, I., Nádosi, M., Harasztos, A., Békés, F., Tömösközi, S. 2011. Lab-on-a-chip technology in cereal science: Analytical properties and possible application areas. Acta Alimentaria (in press).
Bedõ, Z., Vida, Gy., Láng, L., Karsai, I. 1998. Breeding for breadmaking quality using old Hungarian wheat varieties. Euphytica 100:179–182.
Bedõ, Z., Vida, Gy., Láng, L., Juhász, A., Karsai, I. 1999. Breeding a wheat variety with different lines for technological quality and HMW glutenin composition. J. Genet. Breed. 53:57–62.
Békés, F., Kemény, S., Morell, M. 2006a. An integrated approach to predicting end-product quality of wheat. Eur. J. Agron. 25:155–162.
Békés, F., Cavanagh, C.R., Martinov, S., Bushuk, W., Wrigley, C.W. 2006b. The Gluten Composition of Wheat Varieties and Genotypes. Part III. Composition table for HMW-GS. http://www.aaccnet.org/grainbin/pdfs/II_HMW_Subunits.pdf
Békés, F., Cavanagh, C.R., Martinov, S., Bushuk, W., Wrigley, C.W. 2006c. The Gluten Composition of Wheat Varieties and Genotypes. Part II. Composition table for LMW-GS. http://www.aaccnet.org/grainbin/pdfs/III_LMWSubunits.pdf
Branlard, G., Dardevet, M., Amiour, M., Igrejas, G. 2003. Allelic diversity of HMW and LMW glutenin subunits and omegagliadins in French bread wheat (Triticum aestivum L.). Genet. Resour. Crop Evol. 50:669–679.
Cornish, G.B., Békés, F., Eagles, H.A., Payne, P.I. 2006. Prediction of dough properties for bread wheats (Chapter 8). In: Wrigley, C.W., Békés, F., Bushuk, W. (eds), Gliadin and Glutenin. The Unique Balance of Wheat Quality. AACCI Press, St Paul, MN, USA, pp. 243–280.
Eagles, H.A., Hollamby, G.J., Gororo, N.N., Eastwood, R.F. 2002. Estimation and utilisation of glutenin gene effects from the analysis of unbalanced data from wheat breeding programs. Aust. J. Agric. Res. 53:1047–1057.
Gupta, R.B., Shepherd, K.W. 1990. Two-step one-dimensional SDS-PAGE analysis for LMW-GS. 2. Genetic control of the subunits in species related to wheat. Theor. Appl. Genet. 80:65–74.
Gupta, R.B., Békés, F., Wrigley, C.W. 1991. Prediction of physical dough properties from glutenin subunit composition in bread wheats: correlation studies. Cereal Chem. 68:328.
Jackson, E.A., Morel, M.H., Sontag-Sthorm, T., Branlard, G., Metaskovsky, E.V., Redaelli, R. 1996. Characterisation of HMW gliadin and LMW glutenin subunits of wheat endosperm by 2D electrphoresis and the chromosomal localisation of their controlling genes. Theor. Appl. Genet. 66:29–37.
Juhász, A., Larroque, O., Tamás, L., Hsam, S.K.L., Zeller, F., Békés, F., Bedõ, Z. 2003. Bánkúti 1201 - an old Hungarian wheat variety with special storage protein composition. Theor. Appl. Genet. 107:697–704.
Kammholz, S.J., Campbell, A.W., Sutherland, M.W., Hollamby, G.J., Martin, P.J., Eastwood, R.F., Barclay, I., Wilson, R.E., Brennan, P.S., Sheppard, J.A. 2001. Establishment and characterisation of wheat genetic mapping populations. Aust. J. Agric. Res. 52:1079–1088.
Kussmann, M.E., Nordhoff, H., Rahbek-Nielsen, S., Haebel, M., Rossel-Larsen, L., Jakobsen, J., Gobom, E., Mirgorodskaya, A., Kroll-Kristensen, L., Roepstorff, P. 1997. MALDI-MS sample preparation techniques designed for various peptide and protein analytes. J. Mass Spectrom. 32:593–601.
Liu, L., Wang, A., Appels, R., Ma, J., Xia, X., Lan, P., He, Z., Bekes, F., Yan, Y., Ma, W. 2009. A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding. J. Cereal Sci. 50:295–301.
Ma, M., Wang, A., Liu, L., Békés, F., Newberry, M., Gao, L., Ma, J., Islam, S., Yan, Z., He, Y., Xia, X., Appels, R. 2011. High resolution identification of high and low molecular weight glutenin alleles by Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in common wheat (Triticum aestivum L.). In: Branlard, G. (ed.), Proc. 10th International Gluten Workshop, Clermont-Ferrand, France, September 7–9, 2009, pp. 147–151.
Mann, G., Diffey, S., Rampling, L., Nath, A., Kutty, I., Leyne, E., Azanza, F., Cullis, B., Smith, A., Morell, M. 2007. A QTL approach to identifying genes controlling protein, processing and baking quality attributes in wheat. In: Lookhart, G.L., Ng, P.K.W. (eds), Gluten Proteins 2006. AACCI Press, St Paul, USA, pp. 73–77.
Marchylo, B.A., Kruger, J.E., Hatcher, D.W. 1989. Quantitative reverse-phase high performance liquid chromatographic analysis of wheat storage proteins as a potential quality prediction tool. J. Cereal Sci. 9:113–130.
Melas, V., Molel, M.H., Autran, J.C., Feillet, P. 1994. Simple and rapid method for purifying low molecular weight subunits of glutenin from wheat. Cereal Chem. 71:234–237.
Millar, S.J., Snape, J., Ward, J., Shewry, P.R., Belton, P., Boniface, K., Summers, R. 2008. Investigating wheat functionality through breeding and end use (FQS 23) HGCA, Project Report No. 429. Campden and Chorleywood Food Research Association, Chipping Campden, U.K.
Ng, P.K.W., Pogna, N.E., Mellini, F., Bushuk, W. 1989. Glu-1 allele compositions of the wheat cultivars registered in Canada. J. Genet. Breed. 43:53–59.
Payne, P.I., Lawrence, G.J. 1983. Catalogue of alleles for the complex loci Glu-A1, Glu-B1 and Glu-D1 which coded for HMW-GS in hexaploid wheat. Cereal Res. Comm. 11:29–35.
Payne, P.I., Nightingale, M.A., Krattiger, A.F., Holt, L.M. 1987. The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varietes. J. Sci. Food Agric. 40:51–56.
Pogna, N.E., Mellini, F., Beretta, A., Deruffo, A. 1989. The high-molecular-weight glutenin subunits of common wheat cultivars grown in Italy. J. Genet. Breed. 43:17–24.
Rakszegi, M., Kárpáti, M., Lásztity, R., Bedõ, Z. 1999. Study of the LMW glutenin subunits of some old Hungarian wheat cultivars. Cereal Res. Commun. 27:293–299.
Rakszegi, M., Scholz, É., Kárpáti, M., Ganzler, K., Lásztity, R., Bedõ, Z. 2000. Study of the LMW glutenin composition of some old Hungarian wheat cultivars using capillary electrophoresis. Cereal Res. Commun. 28:417–424.
Singh, N.K., Shepherd, K.W., Cornish, G.B. 1991. Asimplified SDS-PAGE procedure for separating LMW subunits of glutenin. J. Cereal Sci. 14:203–208.
Uthayakumaran, S., Listiohadi, Y., Baratta, M., Batey, I.L., Wrigley, C.W. 2006. Rapid identification and quantitation of high-molecular-weight glutenin subunits. J. Cereal Sci. 47:1–6.
Vawser, M.J., Cornish, G.B. 2004. Over-expression of HMWglutenin subunit Glu-B1 7* in hexaploid wheat varieties (Triticum aestivum). Aust. J. Agric. Res. 55:577–588.
Wrigley, C.W., Békés, F., Bushuk, W. 2006. Gluten: A balance of gliadin and glutenin. In: Wrigley, C.W., Békés, F., Bushuk, W. (eds), Gliadin and Glutenin. The Unique Balance of Wheat Quality. AACCI Press, St Paul, MN, USA, pp. 3–33.
Zhen, Z., Mares, D. 1992. Asimple extraction and one-step SDS-PAGE for separating HMW and LMW glutenin subunits of wheat and high molecular weight proteins of rye. J. Cereal Sci. 15:63–78.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by H. Grausgruber
Rights and permissions
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
About this article
Cite this article
Baracskai, I., Balázs, G., Liu, L. et al. A retrospective analysis of HMW and LMW glutenin alleles of cultivars bred in Martonvásár, Hungary. CEREAL RESEARCH COMMUNICATIONS 39, 225–236 (2011). https://doi.org/10.1556/CRC.39.2011.2.6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1556/CRC.39.2011.2.6