Abstract
Six maize hybrids and their parental inbred lines, grown under controlled conditions, were tested for chilling tolerance using the chlorophyll fluorescence induction technique. The genotypes were ranked based on the decrease in the Fv/Fm parameter after chilling stress at 5°C. The activities of enzymes playing a role in stress defence mechanisms (catalase, glutathione reductase, ascorbate peroxidase, guaiacol peroxidase and glutathione-S-transferase) were determined in control plants and after 1 day of cold treatment. The results suggest that although there are differences between the genotypes in the activities of almost all the antioxidant enzymes, these differences do not reflect the differences in the chilling tolerance.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Ádám, A., Bestwick, CS., Barna, B., Mansfield, J.W. (1995) Enzymes regulating the accumulation of active oxygen species during the hypersensitive reaction of bean to Pseudomonas syringae pv. Phaseolicola. Planta 197: 240–249.
Apel, K., Hirt, H. (2004) Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Annu. Rev. Plant Biol. 55: 373–379.
Baker, N.R., East, T.M., Long, S.P. (1983) Chilling damage to photosynthesis in young Zea mays. II. Photochemical function of thylakoids in vivo. J. Exp. Bot. 34: 189–197.
Bridger, G.M., Yang, W., Falk, D.E., McKersie, B.D. (1994) Cold-acclimation increases tolerance of activated oxygen in winter cereals. J. Plant Physiol. 144: 235–240.
Foyer, C.H., Vanacker, H., Gomez, L.D., Harbinson, J. (2002) Regulation of photosynthesis and antioxidant metabolism in maize leaves at optimal and chilling temperatures: review. Plant Physiol. Biochem. 40:659–668.
Holá, D., Langrová, K., Kočcová, M., Rothová, O. (2003) Photosynthetic parameters of maize (Zea mays L.) inbred lines and F-1 hybrids: their different response to, and recovery from rapid or gradual onset of low-temperature stress. Photosynthetica 41: 429–442.
Holá, D., Kočcová, M., Rothová, O., Benečsová, M., Bartáková, J., Wilhelmová, N. (2004) Different response of inbred and hybrid maize to chilling periods of various duration. Acta Physiol. Plant. 26 Suppl.: 227–228.
Janda, T., Szalai, G., Kissimon, J., Páldi, E., Marton, C. and Szigeti, Z. (1994) Role of irradiance in the chilling injury of young maize plants studied by chlorophyll fluorescence induction measurements. Photosynthetica 30:293–299.
Janda, T. (1998) Use of chlorophyll fluorescence induction techniques in the study of low temperature stress in plants. Acta Agron. Hung. 46:77–91.
Janda, T., Szalai, G., Ducruet, J.-M., Páldi, E. (1998) Changes in photosynthesis in inbred maize lines with different degrees of chilling tolerance grown at optimum and suboptimum temperatures. Photosynthetica 35:205–212.
Janda, T., Szalai, G., Rios-Gonzalez, K., Veisz, O., Páldi, E. (2003) Comparative study of frost tolerance and antioxidant activity in cereals. Plant Sci. 164: 301–306.
Kocsy, G., Owttrim, G., Brander, K., Brunold, C. (1997) Effect of chilling on the diurnal rhythm of enzymes involved in protection against oxidative stress in a chilling tolerant and a chilling sensitive maize genotype. Physiol. Plant. 99: 249–254.
Kocsy, G., Galiba, G., Brunold, C. (2001) Role of glutathione in adaptation and signalling during chilling and cold acclimation in plants. Physiol. Plant. 113: 158–164.
Kocsy, G., Kobrehel, K., Szalai, G., Duviau, M.P., Bûzás, Z., Galiba, G. (2004) Abiotic stress-induced changes in glutathione and thioredoxin h levels in maize. Environm. Exp. Bot. 52: 101–112.
Kömerová, M., Holá, D. (1999) The effect of low growth temperature on Hill reaction and photosystem 1 activities and pigment contents in maize inbred lines and their F1 hybrids. Photosynthetica 37:477–488.
Lootens, P., van Waes, J., Carlier, L. (2004) Effect of a short photoinhibition stress on photosynthesis, chlorophyll a fluorescence, and pigment contents of different maize cultivais. Can a rapid and objective stress indicator be found? Photosynthetica 42: 187–192.
Mannervik, B., Guthenberg, G (1981) Glutathione transferase (Human placenta). Methods in Enzymol. 77: 231–235.
Smith, I.K., Vierheller, T.L., Thorne, C.A. (1988) Assay of glutathione reductase in crude tissue homogenates using 5,5’dithiobis(2-nitrobenzoic acid). Anal. Biochem. 175:408–413.
Takáčc, T., Luxová, M., Gačsparíková, O. (2003) Cold induced changes in antioxidant enzymes activity in roots and leaves of two maize cultivars. Biologia, Bratislava, 58: 875–880.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
About this article
Cite this article
Janda, T., Kósa, E., Pintér, J. et al. Antioxidant activity and chilling tolerance of young maize inbred lines and their hybrids. CEREAL RESEARCH COMMUNICATIONS 33, 541–548 (2005). https://doi.org/10.1556/CRC.33.2005.2-3.117
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1556/CRC.33.2005.2-3.117