Abstract
Wheat endosperm storage proteins are the major components of gluten. They play an important role in dough properties and in bread making quality in various wheat varieties. In the present study, the different alleles encoded at the 6 glutenin loci were identified from a set of 71 hexaploid wheat germplasm cultivated in Algeria using SDS-PAGE. At Glu-A1, Glu-B1 and Glu-D1, encoding high molecular weight glutenin subunits (HMW-GS), 3, 6 and 5 alleles were observed, respectively. Low molecular weight glutenin subunits (LMW-GS) displayed similar polymorphism, as 4, 9 and 3 alleles were identified at loci Glu-A3, Glu-B3 and Glu-D3, respectively. A total of 52 patterns resulted from the genetic combination of the alleles encoding at the six glutenin loci. This led to a significantly higher Nei coefficient of genetic variation in Glu-1 and Glu-3 loci (0.54). The Algerian hexaploid wheats exhibited allelic variation in HMW and LMW glutenin subunit composition and the variation differed from that of hexaploid wheats of other countries. The presence of high quality alleles in glutenin loci have led the Algerian wheat cultivars to be considered as an asset in breeding programs aimed for wheat quality.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Autran J.C., Laignelet B., Morel M.H. 1987. Characterization and quantification of LMW glutenins in durum wheats. Biochimie 69:699–711.
Bellil I., Chekara-Bouziani M., Khelifi D. 2012. Genetic diversity of high and low molecular weight glutenin subunits in Saharan bread and durum wheats from Algerian Oases. Czech J. of Genetics and Plant Breeding 48:23–32.
Branlard G., Dardevet M. 1985. Diversity of grain protein and bread wheat quality. 2. Correlation between molecular weight subunits of glutenin and flour quality characteristics. J. Cereal Sci. 3:345–354.
Branlard G., Dardevet M., Amiour N., Igrejas G. 2003. Allelic diversity of HMW and LMW glutenin subunits and omega gliadins in French bread wheat (Triticum aestivum L.). Genetic Resources and Crop Evolution 50:669–679.
Gupta R.B., Singh N.K., Shepherd K.W. 1989. The cumulative effect of allelic variation in LMW and HMW glutenin subunits on dough properties in the progeny of two bread wheats. Theor. Appl. Genet. 77:57–64.
Gupta R.B., Bekes F., Wrigley C.W., Moss H.J. 1990. Prediction of wheat quality in breeding on the basis of LMWandHMWglutenin subunit composition. In: Sixth Assembly of the Wheat Breeding Society of Australia, Tamworth, NSW, Australia, pp. 217–225.
Gupta R.B., Shepherd K.W. 1990. Two-steps one dimensional SDS-PAGE analysis of LMW subunits of glutenin. 1. Variation and genetic control of the subunits in hexaploid wheats. Theor. Appl. Genet. 80:65–74.
Jackson E.A., Holt L.M., Payne P.I. 1983. Characterization of high molecular weight gliadin and low-molecular-weight glutenin subunits of wheat endosperm by two-dimensional electrophoresis and the chromosome allocation of their controlling genes. Theor. Appl. Genet. 66:29–37.
Jackson E.A., Morel M.H., Sontage-Strohm T., Branlard G., Metakovsky E.V., Redaelli R. 1996. Proposal for combining classification systems of alleles of Gli-1 and Glu-3 loci in bread wheat (Triticum aestivum L.). J. Genetic Breeding 50:321–336.
Khelifi D., Branlard G. 1992. The effects of HMW and LMW subunits of glutenins and of gliadins on the technological quality of progeny from our crosses between poor bread making quality and strong wheat cultivars. J. Cereal Sci. 16:195–209.
Nei M. 1973. Analysis of gene diversity in subdivided populations. Proc. National Academy of Sciences of the United States of America 70:3321–3323.
Ng P.K.W., Bushuk W. 1989. Statistical relationships between HMW subunits of glutenin and bread-making quality of Canadian-grown wheats. Cereal Chem. 65:408–413.
Nieto-Taladriz M.T., Perretant M.R., Rousset M. 1994. Effects of gliadins and HMW and LMW subunits of glutenin on dough properties in the F6 recombinant inbred lines from a bread wheat cross. Theor. Appl. Genet. 88:81–88.
Payne P.I. 1987. Genetics of wheat storage proteins and the effect of allelic variation on breadmaking quality. Annual Review of Plant Physiol. 38:141–153.
Payne P.I., Corfield K.G. 1979. Subunit composition of wheat glutenin protein isolated by gel filtration in a dissociating medium. Planta 145:83–88.
Payne P.I., Lawrence G.J. 1983. Catalogues of alleles for the complex gene loci, Glu-A1, Glu-B1 and Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res. Commun. 11:29–35.
Payne P.I., Nightingale M.A., Krattiger A.F., Holt L.M. 1987. The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties. J. of Sciences and Food Agriculture 40:51–65.
Payne P.I., Holt L.M., Jackson E.A., Law C.N., Damania A.B. 1984. Wheat storage proteins: their genetics and their potential for manipulation by plant breeding. Philosophical Transactions of the Royal Society London 304:359–371.
Porceddu E., Turchetta T., Masci S., D’Ovidio R., Lafiandra D., Kasarda D.D., Impiglia A., Nachit M.M. 1998. Variation in endosperm protein composition and technological quality properties in durum wheat. Euphytica 100:197–205.
Shewry P.R., Tatham A.S. 1990. The prolamin storage proteins of cereal seeds: structure and evolution. Biochemistry J. 267:1–12.
Shewry P.R., Halford N.G., Lafiandra D. 2003. Genetics of wheat gluten proteins genetics of wheat gluten proteins. Advances in Genetics 49:111–184.
Singh N.K., Shepherd K.W., Cornish G.B. 1991. Asimplified SDS-PAGE procedure for separatingLMWsubunits of glutenin. J. Cereal Sci. 14:203–208.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by F. Békés
Rights and permissions
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
About this article
Cite this article
Bellil, I., Hamdi, O. & Khelifi, D. Allelic Variation in Glu-1 and Glu-3 Loci of Bread Wheat (Triticum aestivum ssp. aestivum L. em. Thell.) Germplasm Cultivated in Algeria. CEREAL RESEARCH COMMUNICATIONS 42, 648–657 (2014). https://doi.org/10.1556/CRC.2014.0004
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1556/CRC.2014.0004