Abstract
A full length phytase (TaPAPhy_a1.1) gene was cloned and sequenced from Indian wheat cultivar DBW17. The 2,060 bp sequence has four introns of 63, 90, 94, 103 bp and encodes a polypeptide of 548 amino acids. N-terminal residues 5 to 19 contain a signaling peptide (SP) targeted to the vacuole. The predicted molecular weight and isoelectric point were 60.53 kDa and 6.74, respectively. A phylogenetic tree analysis revealed that TaPAPhy_a1.1 clustered on a branch with PAPhy_a1 from Aegilops tauschii cultivar NGB 9855. A structural model of TaPAPhy_a1.1 protein showed a single chain dimer containing two metal ions (Fe and Zn) bound at its active site. It belongs to the metallophosphoesterase group containing a characteristic set of seven amino acid residues (Asp, Asp, Tyr, Asn, His, His, His) involved in metal ligation. The full length TaPAPhy_a1.1 gene sequence from this investigation can be used for allele mining studies to identify superior alleles for higher phytase levels for improving the nutritional quality of wheat.
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Communicated by R.A. McIntosh
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Verma, A., Ram, S. & Dalal, S. Characterization of a Phytase (TaPAPhy_a1.1) Gene in an Indian Wheat Cultivar. CEREAL RESEARCH COMMUNICATIONS 42, 102–110 (2014). https://doi.org/10.1556/CRC.2013.0046
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DOI: https://doi.org/10.1556/CRC.2013.0046