Abstract
Mycelium of the white-rot fungus (Hericium erinaceum (Bull.: Fr. Pers.) produces polysaccharides showing anticancer and immunostimulating activity. In our previous works, we have shown that organic selenitetriglycerides (Selol) contribute to the increase of biosynthesis of exopolysaccharides (EPS) having antioxidative properties and containing large amounts of selenium. The present work is a study of influence of inorganic and organic form of selenium on viability of H. erinaceum mycelium and on ultrastructural changes taking place during its development in submerged culture. The mycelium was grown on media containing sodium selenite (Na2SeO3), a mixture of Na2SeO3 + Selol2% and on control medium (no selenium added). It was shown that mycelium cultured for 3 days in control conditions on standard media contained almost 100% of living cells, with over 80% after 24 days. Treatment with 100 ppm of Na2SeO3 lowered the number of viable cells to 11.8% and 9.1% after 3 and 24 days, respectively. The addition of 2% Selol caused the amounts of living cells to remain at ca 90%. Apparently, Selol helped the cells to cope with the toxic activity of inorganic selenium ions. The addition of sodium selenite induced degradative changes in cell organelles. Such changes were not observed in the case of Na2SeO3 + Selol mixture, in which case cells contained numerous ribosomes and small lipid bodies.
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Alaejos, M. S., Diaz Romero, C. (2000) Selenium and cancer: some nutritional aspects. Nutritio. 16, 376–383.
Chen, T., Zheng, W., Wong, Y. S., Yang, E., Bai, Y. (2006) Accumulation of selenium in mixotrophic culture of Spirulina platensis on glucose. Biores. Technol. 97, 2260–2265.
Chihara, G. (1987) Antitumor and metastasis - inhibitory activites of Lentinan as an immunomodula-tor: an overview. Cancer Detect. Prev. 1, 423–443.
Cybart, A. (2000) Rola selenu w organizmie czlowieka. Żywienie i zdrowie 2.
Filipek, J. (1992) The effect of the mushroom Pleurotus ostreatus on the lipid peroxidation of phosphatidylcholine liposomes. Pharmazie 47, 393.
Fitak, B., Grabowski, M., Suchocki, P. (1999) Pol. Pl 176530 (Cl. A61K31/095), 30 June 1999, Appl. 304046, 29 June 1994. Polski Patent 1999, 6.
Gasmi, A., Gamier, R., Galliot-Guilley, M., Gaudillat, C., Quartenoud, B., Buisine, A., Djebbar, D. (1997) Acute selenium poisoning. Vet. Hum. Toxicol. 39, 304–308.
Jastrzębski, Z., Czyżewska-Szafran, H., Fijalek, Z., Suchocki, P., Fitak, B. A. (1995) Toxicity studies of a new selenium compound, Selol, in rats. Drugs Exp. Clin. Res. 21, 217–220.
Jong-Min, L., Jong-Won, Y. (2006) Enhanced production of exopolysaccharides by supplementation of toluene in submerged culture of an edible mushroom Collybia maculata TG-1. Proc. Bioch. 41, 1620–1626.
Kim, D. H., Yang, B. K., Jeong, S. C., Hur, N. J., Das, S., Yun, J. W. (2001) A preliminary study on the hypoglycemic effect of the exo-polymers produced by five different medicinal mushrooms. J. Microbiol. Biotechnol. 11, 167–171.
Kim, D. H., Yang, B. K., Jeong, S. C., Park, J. B., Cho, S. P., Das, S. (2001) Production of ahypogly-cemic, extracellular polysaccharide from the submerged culture of the mushroom, Phellinus linteus. Biotechnol. Lett. 23, 513–517.
Lanfear, J., Fleming, J., Wu, L., Webster, G., Harrison, P. R. (1994) The selenium metabolite seleno-glutathione induces p53 and apoptosis: relevance to chemioprotective effects of selenium? Carcinogenesi. 51, 1387–1392.
Lee, B. C., Bae, J. T., Pyo, H. B., Choe, T. B., Kim, S. W., Hwang, H. J. (2003) Biological activities of the polysaccharides produced from submerged culture of the edible Basidiomycete Grifolafron-dosa. Enzyme Microb. Technol. 32, 574–581.
Liu, F., Ooi, V. E., Chang, S. T. (1997) Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci. 60, 763–771.
Malinowska, E., Krzyczkowski, W., Herold, F., Łapienis, G., Ślusarczyk, J., Suchocki, P., Kuraś, M., Turło, J. (2009) Biosynthesis of selenium-containing polysaccharides with antioxidant activity in liquid culture of Hericium erinaceum. Enzyme Microb. Technol. 44, 334–343.
Mizuno, T. (1999) Bioactive substances in Hericium erinaceus (Bull.: Fr.) Pers. (Yamabushitake), and its medicinal utilization. Int. J. Med. Mush. 1, 105–119.
Muller, W. C., Greenwood, A. D. (1978) The ultrastructure of phenolic - strong cells fixed with caffeine. J. Exper. Bot. 110, 757–764.
Rayman, M. P. (2000) The importance of selenium to human health. Lance. 356, 233–241.
Reynolds, E. S. (1963) The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17, 208–212.
Shen, H., Yang, C., Liu, J., Ong, C. (2000) Dual role of glutathione in selenite-induced oxidative stress and apoptosis in human hepatoma cells. Free Radic. Biol. andMe. 286, 1115–1124.
Stasinopoulos, S. J., Seviour, R. J. (1990) Stimulation of exopolysaccharide production in the fungus Acremonium persicinum with fatty acids. Biotechnol. Bioeng. 36, 778–782.
Stewart, M. S., Davis, R. D., Walsh, L. P., Pence, B. C. (1997) Induction of differentiation apoptosis by sodium selenite in human colonic carcinoma cells (HT 29). Cancer Lett. 117, 350–400.
Suchocki, P., Misiewicz, I., Skupihska, K., Waclawek, K., Fijalek, Z., Kasprzycka-Guttman, T. (2007) The activity of Selol in multidrug-resistant and sensitive human leukemia cells. Oncol. Rep. 18, 893–899.
Wasser, S. P. (2002) Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl. Microbiol. Biotechnol. 60, 258–274.
Whanger, P. D. (2004) Selenium and its relationship to cancer: an update. Brit. J. Nutr. 91, 11–28.
Wolf, C., Lewis, A., Adams, D., Allan, D., Ansel, D. (1997) The role glutathione in determining the response of normal and tumors cells to anticancer drugs. Biochem. Soc. Trans. 15, 728–730.
Zagrodzki, P., Bik, D., Fitak, B., Suchocki, P., Niemczuk, K. (2000) Selenoenzymes in animal tissues after supplementation with Selol. Bull. Vet. Inst. Pulaw. 44, 215–220.
Zhang, A., Sun, P., Zhang, J., Tang, C., Fan, J., Shi, X. (2007) Structural investigation of a novel fucoglucogalactan isolated from the fruitingbodies of the fungus Hericium erinaceus. Food Chem. 104, 451–456.
Zhu, Z., Kimura, M., Itokawa, Y., Nakatsu, S., Oda, Y., Kikuchi, H. (1995) Effect of selenium on malignant tumor cells of brain. Biol. Trace Elem. Res. 49, 1–7.
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Ślusarczyk, J., Malinowska, E., Krzyczkowski, W. et al. Influence of Inorganic and Organic Selenium on Number of Living Mycelial Cells and Their Ultrastructure in Culture of Hericium erinaceum (Bull: Fr. Pers.). BIOLOGIA FUTURA 64, 96–105 (2013). https://doi.org/10.1556/ABiol.64.2013.1.9
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DOI: https://doi.org/10.1556/ABiol.64.2013.1.9