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Induction of Antioxidant Activity by Selenium Compounds in the Aspergillus niger Mycelium

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Abstract—

Investigation of the induction of antioxidant activity (AOA) by selenium compounds (Na2SeO3, diacetophenonyl selenide (DAPS-25), L-selenocystine) in various organisms is of interest as a way of protecting cell membranes from oxidative stress. A comparative analysis of the antioxidant activity of 23 amino acids was performed by coulometric titration with electrogenerated bromine and iodine. The activity decreases in the order: cystine > tryptophan > selenocystine > tyrosine > 3,3'-dimethyl-L-selenocystine > methionine. Only amino acids with sulfhydryl and selenol groups as more active reductants can interact with the electrogenerated iodine: cysteine > selenocysteine > threo-3-methyl-L-selenocysteine. Probably the correction of the antioxidant status at the amino acids level is based on the sulfhydryl and selenol groups in radicals. In case they are not enough, cystine, selenocystin, tryptophan, tyrosine, and methionine will act as scavenger-reductants. It was found that selenium compounds dose-dependently induce the total antioxidant activity of the Aspergillus niger mycelium and affect indicators of the antioxidant status (amino acid composition and catalase activity), which in turn stimulates the biomass accumulations. DAPS-25 and sodium selenite treatment at high doses (0.025 mg Se/L) caused the greatest effect on the total AOA induction (3.4–5.5 times). Lower concentrations (0.0025–0.00025 mg Se/L) had a lesser effect (25.8–41.7%). Activity in samples with L-selenocystin increased by 1.6–43.3%. It is noted that the iodine antioxidant activity in the mycelium was generally lower than the bromine one.

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Authors and Affiliations

  1. Penza State University, 440026, Penza, Russia

    P. A. Poluboyarinov, A. V. Kuznetsova, I. Ya. Moiseeva & N. I. Mikulyak

  2. MIREA—Russian Technological University (Lomonosov Institute of Fine Chemical Technologies), 125190, Moscow, Russia

    A. P. Kaplun

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Correspondence to P. A. Poluboyarinov.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Abbreviations: GPX, glutathione peroxidase, DAPS-25, diacetophenonylselenide.

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Poluboyarinov, P.A., Kuznetsova, A.V., Moiseeva, I.Y. et al. Induction of Antioxidant Activity by Selenium Compounds in the Aspergillus niger Mycelium. Russ J Bioorg Chem 49, 823–835 (2023). https://doi.org/10.1134/S1068162023040155

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