Abstract
A new bacterial strain, capable of degrading poly(3-hydroxybutyrate) (PHB) was isolated from soil. This organism, identified as Streptoverticillium kashmirense AF1, secreted PHB depolymerases both on solid as well as in liquid mineral salt medium containing poly(3-hydroxybutyrate) as sole carbon source. The optimum production of PHB depolymerase was observed at pH 8 and 7, at 45 °C, 1% substrate concentration and in the presence of lactose as an additional carbon source. The extracellular PHB depolymerase was purified by gel permeation chromatography using Sephadex G-75. The Streptoverticillium kashmirense AF1 produced two types of PHB depolymerases having molecular weights of about 37 and 45 kDa as determined by SDS-PAGE. The difference in dry cell mass and amount of CO2 evolved in the test and control calculated gravimetrically through Sturm test indicated the degradative capabilities of Streptoverticillium kashmirense AF1.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Abou-Zeid, D. M., Müller, R. J., Deckwer, W. D. (2001) Degradation of natural and synthetic polyesters under anaerobic conditions. J. Biotechnol. 86, 113–126.
Briese, B. H., Schmidt, B., Jendrossek, D. (1994) Pseudomonas lemoignei has five poly(hydroxy-alkanoic acid) (PHA) depolymerase genes: a comparative study of bacterial and eukaryotic PHA depolymerases. J. Environ. Polym. Degrad. 2, 75–87.
Calabia, B. P., Tokiwa, Y. (2006) A novel PHB depolymerase from a thermophilic Streptomyces sp. Biotechnol. Lett. 28, 383–388.
Ghanem, N. B., Mabrouk, M. E., Sabry, S. A., El-Badan, D. E. (2005) Degradation of polyesters by a novel marine Nocardiopsis aegyptia sp. nov.: application of Plackett-Burman experimental design for the improvement of PHB depolymerase activity. J. Gen. Appl. Microbiol. 51, 151–158.
Holt, G. (1993) Bergey’s Manual of Determinative Bacteriology, 9th ed., The Williams and Wilkins Company, Baltimore.
Imam, S. H., Gordon, S. H., Shogren, R. L., Tosteson, T. R., Govind, N. S., Greene, R. V. (1999) Degradation of starch-poly(β-hydroxybutyrate-co-β-hydroxyvalerate) bioplastic in tropical coastal waters. Appl. Environ. Microbiol. 65, 431–437.
Kim, M. N., Lee, B. Y., Lee, I. M., Lee, H. S., Yoon, J. S. (2001) Toxicity and biodegradation of products from polyester hydrolysis. J. Environ. Sci. Health A. Tox. Hazard. Subst. Environ. Eng. 36, 447–463.
Kobayashi, T, Sugiyama, A., Kawase, Y, Saito, T, Mergaert, J., Swings, J. (1999) Biochemical and genetic characterization of an extracellular poly(3-hydroxybutyrate) depolymerase from Acidovorax sp. strain TP4. J. Environ. Polym. Degrad. 7, 9–17.
Manna, A., Paul, A. K. (2000) Degradation of microbial polyester poly(3-hydroxybutyrate) in environmental samples and in culture. Biodegradation 11, 323–329.
Manna, A., Giri, P., Paul, A. K. (1999) Degradation of poly(3-hydroxybutyrate) by soil strepto-mycetes. World J. Microbiol. Biotechnol. 15, 705–709.
Mergaert, J., Webb, A., Anderson, C., Wouters, A., Swings, J. (1993) Microbial degradation of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrateco-3-hydroxyvalerate) in soils. Appl. Environ. Microbiol. 59, 3233–3238.
Mergaert, J., Wouters, A., Swings, J. (1994) Estimation of the intrinsic biodiversity among poly(3-hydroxyalkanoates) degrading streptomycetes using gas chromatographic analysis of fatty acids. Syst. Appl. Microbiol. 17, 601–612.
Mergaert, J., Wouters, A., Anderson, C., Swings, J. (1995) In situ biodegradation of poly(3-hydroxy-butyrate) and poly(3- hydroxybutyrate-co-3-hydroxyvalerate) in natural waters. Can. J. Microbiol. 41, 154–159.
Mergaert, J., Schirmer, A., Hauben, L., Mau, M., Hoste, B., Kerstens, K., Jendrossek, D., Swings, J. (1996) Isolation and identification of poly(3-hydroxy valerate)-degrading strains of Pseudomonas lemoignei. Int. J. Syst. Bacteriol. 46, 769–773.
Mukai, K., Doi, Y., Sema, Y., Tomita, K. (1993) Substrate specificities in hydrolysis of polyhydroxy-alkanoates by microbial esterases. Biotechnol. Lett. 15, 601–604.
Muller, R. J., Augusta, J., Pantke, M. (1992) An Interlaboratory investigation into biodegradation of plastics; Part 1: A modified Sturm-test. Mat. Organ. 27, 179–189.
Nishida, H., Tokiwa, Y (1993) Distribution of poly(P-hydroxybutyrate) and poly(e-caprolactone) aerobic degrading microorganisms in different environments. J. Environ. Polym. Degrad. 1, 227–233.
Nojiri, M., Saito, T. (1997) Structure and function of poly(3-hydroxybutyrate) depolymerase from Alcaligenes faecalis Tl. J. Bacteriol. 179, 6965–6970.
Pagga, U., Beimborn, D. B., Boelens, J., De Wilde, B. (1995) Determination of the aerobic biodegradability of polymeric material in a laboratory controlled composting test. Chemosphere 31, 4475–4487.
Pagga, U., Schafer, A., Muller, R. J., Pantke, M. (2001) Determination of the aerobic biodegradability of polymeric material in aquatic batch tests. Chemosphere 42, 319–331.
Reinhardt, S., Handrick, R., Jendrossek, D. (2002) The “PHB depolymerase inhibitor” of Pauci-monas lemoignei is a PHB depolymerase. Biomacromolecule 3, 823–827.
Romen, F., Reinhardt, S., Jendrossek, D. (2004) Thermotolerant poly(3-hydroxybutyrate)-degrading bacteria from hot compost and characterization of the PHB depolymerase of Schlegelella sp. KBla. Arch. Microbiol. 182: 157–164.
Schirmer, A., Jendrossek, D., Schlegel, H. G. (1993) Degradation of poly(3-hydroxyoctanoic acid) [P(3HO)] by bacteria: purification and properties of a P(3HO) depolymerase from Pseudomonas fluorescens GK13. Appl. Environ. Microbiol. 59, 1220–1227.
Shah, A. A., Hasan, F., Hameed, A., Ahmed, S. (2007) Isolation and characterization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) degrading bacteria and purification of PHBV depolymerase from newly isolated Bacillus sp. AF3. Int. Biodeterior Biodegrad. 60, 109–115.
Tansengco, M. L., Tokiwa, Y (1998) Thermophilic microbial degradation of polyethylene succinate. World J. Microbiol. Biotechnol. 14, 133–138.
Tokiwa, Y., Calabia, B. P. (2004) Degradation of microbial polyesters. Biotechnol. Lett. 26, 1181–1189.
Whitchurch, G. J., Cooper, T. A. (2006) Thermoplastically-processable biodegradable polyvinyl alcohol biodegradable polyvinyl alcohol-, starch starch- and polylactide and polylactide-based polymer blends. International Degradable Plastics Symposium Chicago IL, USA, https://doi.org/www.plasticsin-dustry.org/membersonly/industry/symposium 2006.
Yamada, K., Mukai, K., Doi, Y. (1993) Enzymatic degradation of poly-(hydroxy-alkanoates) by Pseudomonas pickettii. Int. J. Biol. Macromol. 15, 215–220.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
About this article
Cite this article
Shah, A.A., Hasan, F., Hameed, A. et al. A Novel Poly(3-Hydroxybutyrate)-Degrading Streptoverticillium Kashmirense AF1 Isolated from Soil and Purification of Phb-Depolymerase. BIOLOGIA FUTURA 59, 489–499 (2008). https://doi.org/10.1556/ABiol.59.2008.4.9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1556/ABiol.59.2008.4.9