Abstract
Changes in relative water content (RWC), lipid peroxidation, proline and antioxidant system in relation to the tolerance to oxidative stress enzymes mediated high pressure were investigated in Hordeum vul-gare L. cv. Tokak. For this purpose, mature embryos cultured on MS media were treated in a hyperbaric oxygenation chamber (approx. 59.06 feets, 2 kp/cm2) with pure oxygen for 60 minutes/day for a growth period of ten days in a plant growth chamber. Constitutive activities of SOD, APOX, GR and POX were higher in hyperbaric oxygenated (HBO) explants, being 96.07%, 28.57%, 77.77% and 54.14% for the 5th days; 95.78%, 40%, 37.5%, and 94.98% for the 10th days of culture, respectively, than in the control plants. Increase in SOD activity was also shown on polyacrilamide gel electrophoresis on the 10th day of application. Proline accumulation was increased in HBO-treated explants both on the 5th days (85.71%) and 10th days (37.14%) of treatment. MDA content was found to be higher in HBO treated explants both on the 5th (53.84%) and 10th (59.83%) days of culture.
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Abbreviations
- APOX:
-
ascorbate peroxidase
- CAT:
-
catalase
- GR:
-
glutathione reductase
- HBO:
-
hyperbaric oxygenation
- MDA:
-
malondialdehyde
- MS:
-
Murashige and Skoog medium
- POX:
-
peroxidase
- RWC:
-
relative water content
- SOD:
-
superoxide dismutase
References
Acar, O., Türkan, L., Özdemir, F. (2001) Superoxide dismutase and peroxidase activities in drought sensitive and resistant barley (Hordeum vulgare L.) varieties. Acta Physiol. Plant. 3, 351–356.
Al-Karaki, G. N., Clark, R. B., Sullivan, C. Y. (1996) Phosphorus nutrition and water stress effects on proline in sorghum and bean. J. Plant Physiol. 148, 745–751.
Alscher, R. G. (1989) Biosynthesis and antioxidant properties of glutathione in plants. Physiol. Plant. 77, 457–464.
Asada, K. (1992) Ascorbate peroxidase-α hydrogen scavenging enzyme in plants. Physiol. Plant. 85, 235–241.
Aspinall, D., Paleg, L. G. (1981) Proline accumulation physiological aspects. In: Paleg, L., Aspinall, D. The Physiology and Biochemistry of Drought Resistance in Plants. Academic Press. Sidney, pp. 215–228.
Avecedo, E. (1987) Gas exchange of barley and wheat genotypes under drought. In: Creal Improvement Program Annual Report. ICARDA, Aleppo, Syria, pp. 101–116.
Aydın, A., Orhan, H., Sayal, A., Özata, M., Şahin, G., Işımer, A. (2001) Oxidative stress and nitric oxide related parameters in type II diabetes mellitus: effects of glycemic control. Clin. Biochem. 34, 64–70.
Badiani, M., Biasi, M. G., Colognola, M., Artemi, F. (1990) Catalase, peroxidase and superoxide dis-mutase activities in seedlings submitted to increasing water deficit. Agrochemica, 34, 90–102.
Baisak, R., Rana, D., Acharya, P. B. B., Kar, M. (1994) Alteration in the activities of active oxygen scavenging enzymes of wheat leaves subjected to water stress. Plant Cell Physiol. 35, 489–495.
Barron, M. C., De-Mejia, E. G. (1998) Comparative study of enzymes related to proline metabolism in tepary bean (Phaseolus acutifolius) and common bean (Phaseolus vulgaris) under drought and irrigated conditions. Plant Food Human Nutr. 52, 119–132.
Bates, L. S., Waldren, R. P., Teare, I. D. (1973) Rapid determination of free proline for water-stress studies. Plant Soil. 39, 205–207.
Beauchamps, C., Fridovich, I. (1971) Superoxide dismutase: improved assays and applicable to acry-lamide gels. Anal. Biochem. pp. 248–254.
Bergmeyer, N. (1970) Methoden der enzymatischen Analyse. Vol. 1. Akademie Verlag, Berlin.
Blumm, A. (1988) Plant Breeding for Stress Environments. CRC Press, Boca Raton, F. L.
Bor, M., Ozdemir, F., Türkan, İ (2003) The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet Beta vulgaris L. Plant Sci. 164, 77–84.
Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.
Ceccarelli, S., Grando, S., Impiglia, A. (1998) Choice of selection strategy in breeding barley for stress environments. Euphytica 103, 307–318.
Dennog, C., Radermacher, P., Barnett, A. Y., Speit, G. (1999) Antioxidant status in humans after exposure to hyperbaric oxygen. Mutat. Res. 428, 83–89.
Edreva, A. (2005) Generation and scavenging of reactive oxygen species in chloroplast: a submole-cular approach. Agriculture, Ecosystem and Environment 106, 119–133.
Eken, A., Aydın, A., Sayal, A., Üstündağ, A., Duydu, Y., Dündar, K. (2005) The effects of hyperbaric oxygen treatment on oxidative stress and SCE frequencies in humans. Clinical Biochemistry 38, 1133–1137.
Fadzilla, N. M., Finch, R. P., Burdon, R. H. (1997) Salinity, oxidative stress and antioxidant responses in shoot cultures of rice. J. Exp. Bot. 48, 325–331.
Foyer, C. H., Halliwell, B. (1976) Presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. Planta 133, 21–25.
Fridovich, I. (1986) Biological effects of superoxide radicals. Arach. Biochem. Biophys. 247, 1–11.
Fu, J., Huang, B. (2001) Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environ. Exp. Bot. 45, 105–114.
Gamble, P. E., Burke, J. J. (1984) Effect of water stress on the chloroplast antioxidant system 1. Alterations in glutathione reductase activity. Plant Physiol. 76, 615–621.
Gueta-Dehan, Y., Yaniv, Z., Zilinskas, B. A., Ben-Hayyim, G. (1997) Salt and oxidative stress: similar and specific responses and their relation to salt tolerance in citrus. Planta 203, 460–469.
Gus’kov, E. P., Dvorkina, R. M., Goncharenko, I. I. (1982) Effect of hyperbaric oxygenation on the mitosis of plant root cells. Tsilogia 24, 257–263.
Halliwell, B. (1987) Oxidative damage, lipid peroxidation, and antioxidant protection in chloroplast. Chem Phys. Lipids 44, 327–340.
Hare, P. D., Cress, W. A. (1999) Proline synthesis and degradation a model system foe eluciding stress-related signal transduction. J. Exp. Bot. 50, 413–434.
He, C., Davies, F. T., Lacey, R. E., Drew, M. C., Brown, D. L. (2003) Effect of hypobaric conditions on ethylene evolution and growth of lettuce and wheat. J. Plant Physiol. 160, 1341–1350.
Hernandez, J. A., Olmos, E., Corpas, F. J., Sevilla, F., del Rio, L. A. (1995) Salt-induced oxidative stress in chloroplast of pea plants. Plant Sci. 105, 151–167.
Jain, M., Mathur, G., Koul, S., Sarin, N. B. (2001) Ameliorative effects of proline on salt stress induced lipid peroxidation in cell lines of groundnut (Arachis hypogea L.). Plant Cell Report 20, 463–468.
Jeffrey, T. R., Corey, K. A., Paul, A. L., Ferl, R. J., Wheeler, R.M., Schuerger, A. C. (2006) Exposure of Arabidopsis thaliana to hyperbaric environments: implications fot low-pressure bioregenerative life support systems for human exploration missions and terrafroming on mars. Astrobiology 6, 851–866.
Luna, M., Badiani, M., Felice, M., Artemi, F., Germanni, F. (1985) Selective enzyme inactivation under water stress in maize (Zea mays L.) and wheat (Triticum aestivum L.) seedlings. Environ. Exp. Bot. 25, 153–156.
Madhava, R. K. V., Sresty, T. V. S. (2000) Antioxidative parameters in the seedlings of pigeonpea (Cajanus cajan L. Millspaugh) in response to Zn and Ni stresses. Plant Sci. 157, 113–128.
Mashkina, E., Osatov, A., Danilenko, V., Kolokolova, N., Gus’kov, E. (2006) Responses of sunflower chlorophyll mutants to increased temperature and oxidative burst. Russian Journal of Plant Physiology 53, 205–210.
Murashige, T., Skoog, F. A. (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plantarum 15, 473–497.
Nakano, Y., Asada, K. (1981) Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 22, 867–880.
Nayyar, H., Gupta, D. (2006) Differential sensitivity of C3 and C4 plants to water deficit stress: Association with oxidative stress and antioxidants. Environmental and Environmental Botany 58, 106–113.
Pandey, P. K. (1982) Free proline accumulation in response to water stress in wheat seedling. Curr Sci. 51, 141–143.
Pastori, G. M., Trippi, V. S. (1993) Antioxidative protection in drought-resistance maize strains during leaf senescence. Physiol. Plant 87, 227–231.
Rothfuss, A., Stahl, W., Radermacher, P., Speit, G. (1999) Evaluation of mutagenic effects of hyperbaric oxygen (HBO) in vitro. Environ. Mol. Mutagen. 34, 291–296.
Qurtacci, M. F., Navarri-Izzo, F. (1992) Water stress and free radical mediated changes in sunflower seedlings. J. Plant Physiol. 139, 621–625.
Rabinowitch, H. D., Fridovih, I. (1983) Superoxide radical, superoxide dismutase and oxygen toxicity in plants. Phytochem. Photobiol. 188, 206–213.
Sairam, R. K., Shukla, D. S., Saxena, D. C. (1997) Stress induced injury and antioxidant enzymes in relation to drought tolerance in wheat genotypes. Biologia Plantarum 40, 357–364.
Scandalios, J. G. (1993) Oxygen stress and superoxide dismutases. Plant Physiol. 101, 7–12.
Sgherri, C. L. M., Maffei, M., Navari-Izzo, F. (2000) Antioxidative enzymes in wheat subjected to increasing water deficit and rewatering. J. Plant Physiol. 273–279.
Shang, F., Gong, X., Egtesadi, S. (2002) Vitamin C prevents hyperbaric oxygen induced growth retardation and lipid peroxidation and attenuates the oxidation-induced up-regulation of glutathione in guinea pigs. J. Nutr. Biochem. 13, 307–313.
Smart, R. E., Bingham, G. E. (1974) Rapid estimates of relative water content. Plant Physiol. 53, 258–260.
Smirnoff, N. (1996) The function and metabolism of ascorbic acid in plants. Ann. Bot. 78, 661–669.
Speit, G., Dennog, C., Radermacher, P., Rothfuss, A. (2002) Genotoxicity of hyperbaric oxygen. MutatRes. 2, 1–9.
Wood, Z. (2002) Hyperbaric oxygen in the management of chronic wounds. Br. J. Nursing 11, 17–24.
Zaifnejad, M., Clark, R. B., Sullivan, C. Y. (1997) Aluminum and water stress effects on growth and proline of Sorghum. J. Plant Physiol. 150, 338–344.
Zhang, J., Kirkham, M. B. (1996) Antioxidant responses to drought in sunflower and sorghum seedlings. New Phytol. 132, 361–373.
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Arican, E., Gozukirmizi, N. Effects of Hyperbaric Oxygenation on Cultured Barley Embryos. BIOLOGIA FUTURA 59, 453–464 (2008). https://doi.org/10.1556/ABiol.59.2008.4.6
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DOI: https://doi.org/10.1556/ABiol.59.2008.4.6