Abstract
Drought resistance of bean landraces was compared in order to select genotypes with either high morphological or high biochemical-physiological plasticity. The lines in the former group exhibited fast reduction in fresh and dry mass, decreased the water potential in primary leaves after irrigation withdrawal and the biomass mobilized from the senescent primary leaves was allocated into the roots. These genotypes had high frequency of primary leaf abscission under water stress. The genotypes with plasticity at the biochemical level maintained high water potential and photochemical efficiency, i.e. effective quantum yield, high photochemical (qP) and low non-photochemical (NPQ) quenching in primary leaves under drought stress. While superoxide dismutase activity was not influenced by the drought and the genotype, catalase activity increased significantly in the primary leaves of the genotypes with efficient biochemical adaptation. Lines with high morphological plasticity exhibited higher quaiacol peroxidase activity under drought. Proline may accumulate in both cases, thus it may be a symptom of protein degradation or a successful osmotic adaptation. On the basis of contrasting responses, the genetic material cannot be screened for a large-scale breeding program by a single physiological parameter but by a set of the methods presented in this work.
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Acosta-Gallegos, J. A., Ocoa-Marquez, R., Arrieta-Montiel, M. P., Ibarra-Perez, F., Pajarito-Ravelero, A., Sanchez-Valdez, I. (1995) Registration of Pinto Villa common bean. Crop Sci. 35, 1211–1212.
Bates, L.-S., Waldren, R. P., Teare, L. D. (1973) Rapid determination of free proline for water-stress studies. Plant Soil. 39, 205–207.
Bilger, W., Björkman, O. B. (1990) Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absorbance changes, fluorescence and photosynthesis i. Hedera canarensis. Photosynth. Res. 25, 173–185.
Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.
Brestic, M., Cornic, G., Fryer, M. J., Baker, N. R. (1995) Does photorespiration protect the photo-synthetic apparatus in french bean leaves from photoinhibition during drought stress. Planta 196, 450–470.
Dhindsa, R. S., Plumb-Dhindsa, P., Thorpe, T. A. (1981) Leaf senescence correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismu-tase and catalase. J. Exp. Bot. 32, 93–101.
Flexas, J., Medrano, H. (2002) Energy dissipation in C3 plants under drought. Funct. Plant Biol. 29, 1209–1215.
Fracheboud, Y., Haldimann, P., Leipner, J., Stamp, P. (1999) Chlorophyll fluorescence as a selection tool for cold tolerance of photosynthesis in maiz. (Zea mays L.). J. Exp. Bot. 50, 1533–1540.
Genty, B., Briantais, J. M., Baker, N. R. (1989) The relationship between the quantum yield of pho-tosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim. Biophys. Acta 990, 87–92.
Lichtenthaler, H. K. (1988) In vivo chlorophyll fluorescence as a tool for stress detection in plants. In: Lichtenthaler, H. K. (ed.. Applications of Chlorophyll Fluorescence. Kluwer Academic Publishers, Dordrecht, pp. 143–149.
Lizana, C., Wentworth, M., Martinez, J. P., Villegas, D., Meneses, R., Murchie, E. FL, Pastenes, C., Lercari, B., Vernieri, P., Horton, P., Pinto, M. (2006) Differential adaptation of two varieties of common bean to abiotic stress. I. Effects of drought on yield and photosynthesis. J. Exp. Bot. 57, 658–697.
Madosa, E. (2003) Collection of the plant material and agronomic studies on the local population of vegetable species. In: Erdei, L. (ed.). Characterization of Plant Genetic Resources. Szeged-Timisoara, 2003. PHARE CBC HU-00009-03-01-014 Project Final Report, pp. 196–220.
Schneider, K. A., Rosales-Serna, R., Ibarra-Perez, F., Cazares-Enriquez, B., Acosta-Gallegos, J. A., Ramirez-Vallejo, P., Wassimi, N., Kelly, J. D. (1997) Improving common bean performance under drought stress. Crop Sci. 37, 43–50.
Schreiber, U., Schliwa, U., Bilger, W. (1986) Continuous recording of photochemical and non-photochemical quenching of chlorophyll fluorescence with a new type of modulation fluorometer. Photosynth. Res. 10, 51–62.
Tari, I. (2003/2004) Abaxial and adaxial stomatal density, stomatal conductances and water status of bean primary leaves as affected by paclobutrazol. Biol. Plant. 43, 637–640.
Zlatev, Z. S., Yordanov, I. T (2004) Effects of soil drought on photosynthesis and chlorophyll fluorescence in bean plants. Bulg. J. Plant Physiol. 30, 3–18.
Zlatev, Z. S., Lidon, F. C., Ramalho, J. C., Yordanov, I. T (2006) Comparison of resistance to drought of three bean cultivars. Biol. Plant 50, 389–394.
Upadhaya, A., Sankhla, D., Davis, T. D., Smith, B. N. (1985) Effect of paclobutrazol on the activities of some enzymes of activated oxygen metabolism and lipid peroxidation in senescing soybean leaves. J. Plant Physiol. 121, 453–461.
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Tari, I., Camen, D., Coradini, G. et al. Changes in Chlorophyll Fluorescence Parameters and Oxidative Stress Responses of Bush Bean Genotypes for Selecting Contrasting Acclimation Strategies Under Water Stress. BIOLOGIA FUTURA 59, 335–345 (2008). https://doi.org/10.1556/ABiol.59.2008.3.7
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DOI: https://doi.org/10.1556/ABiol.59.2008.3.7