Abstract
Cultured plant cells of Marchantia polymorpha were examined for their ability to reduce β-keto ester, 2-methyl-3-oxobutanoate. The cells reduced ethyl 2-methyl-3-oxobutanoate to predominantly yield the anti-product, ethyl (2S,3S)-3-hydroxy-2-methylbutanoate, with 92% diastereomeric excess and over 99% enantiomeric excess. The use of immobilized cells of M. polymorpha in calcium alginate gel improved the diastereomeric excess of the product (97% de). In addition, the large-scale reduction of 75 g of ethyl 2-methyl-3-oxobutanoate with immobilized M. polymorpha gave the product with 97% de and >99% ee in 92% yield.
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Shimoda, K., Kubota, N., Nakajima, N. et al. High Production of (2S,3S)-3-Hydroxy-2-Methylbutanoate by Immobilized Plant Cells of Marchantia Polymorpha. BIOLOGIA FUTURA 58, 219–223 (2007). https://doi.org/10.1556/ABiol.58.2007.2.8
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DOI: https://doi.org/10.1556/ABiol.58.2007.2.8