Abstract
Recent and historical evidence is consistent with the view that atherosclerosis is an infectious disease or microbial toxicosis impacted by genetics and behavior. Because small bacterial-like particles, also known as nanobacteria have been detected in kidney stones, kidney and liver cyst fluids, and can form a calcium apatite coat we posited that this agent is present in calcified human atherosclerotic plaques. Carotid and aortic atherosclerotic plaques and blood samples collected at autopsy were examined for nanobacteria- like structures by light microscopy (hematoxylin-eosin and a calcium-specific von Kossa staining), immuno-gold labeling for transmission electron microscopy (TEM) for specific nanobacterial antigens, and propagation from homogenized, filtered specimens in culture medium. Nanobacterial antigens were identified in situ by immuno-TEM in 9 of 14 plaque specimens, but none of the normal carotid or aortic tissue (5 specimens). Nanobacteria-like particles were propagated from 26 of 42 sclerotic aorta and carotid samples and were confirmed by dot immunoblot, light microscopy and TEM. [3H]L-aspartic acid was incorporated into high molecular weight compounds of demineralized particles. PCR amplification of 16S rDNA sequences from the particles was unsuccessful by traditional protocols. Identification of nanobacteria-like particles at the lesion supports, but does not by itself prove the hypothesis that these agents contribute to the pathogenesis of atherosclerosis, especially vascular calcifications.
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Abbreviations
- DMEM:
-
Dulbecco’s Eagle’s medium
- TEM:
-
transmission electron microscopy
- NB:
-
nanobacteria
- EM:
-
electronmicroscopy
- FBS:
-
fetal bovine serum
- LDL:
-
low density lipoprotein
- EDTA:
-
ethylene-diamino-tetraacetate
- BSA:
-
bovine serum albumin
- PBS:
-
phosphate-buffered saline
- FITC:
-
fluorescein-isothiocyanate
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Puskás, L.G., Tiszlavicz, L., Rázga, Z. et al. Detection of Nanobacteria-Like Particles in Human Atherosclerotic Plaques. BIOLOGIA FUTURA 56, 233–245 (2005). https://doi.org/10.1556/ABiol.56.2005.3-4.7
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DOI: https://doi.org/10.1556/ABiol.56.2005.3-4.7