Abstract
Effects of embelin on oestrous cycle, plasma levels of progesterone and oestradiol, and in vitro production of oestradiol and progesterone by mixed ovarian cells was studied. Forty adult (4 months old) regularly cycling female Sprague-Dawley rats were divided into four groups of 10 rats each. Groups I and II (controls) were given 1 ml/kg body weight of physiological saline or corn oil (vehicle). Groups III and IV received 10 mg/kg and 20 mg/kg body weight embelin in corn oil, respectively. Emberlin disrupted the oestrous cycles in Groups III and IV animals, and there was a significant depression in plasma oestra-diol (p < 0.05) and progesterone (p < 0.02) at both 10 and 20 mg/kg body weights, respectively. Isolated mixed ovarian cells from embelin treated rats produced significantly less progesterone and estradiol than controls in vitro. It is concluded that embelin probably interferes with reproductive functions in female rats by suppressing ovarian production of sex steroid hormones.
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References
Andersen, J. M., Dietschy, J. M. (1978) Relative importance of high and low density lipoproteins in the regulation of cholestrol synthesis in the adrenal gland, ovary, and testis of the rat. J. Biol. Chem. 253, 9024–9030.
Bargava, S. R., Dixit, V. P. (1985) Anti-fertility effects of embelin and plumbagin in female rats. Plant Med. 19, 29–34.
Bogh, H. O., Andreasen, J., Lemmich, J. (1996) Anti-helminthic usage of extracts of embelin from Tanzania. J. Ethnopharmacology 50, 35–42.
Githiu, E. K., Makawiti, D. W., Midiwo, J. O. (1991) Changes in the concentration of testosterone, luteinising hormone and progesterone associated with the administration of embelin. Contraception 44, 311–317.
Gupta, S., Sample, S. N., Kammar, U. (1990) Changes in glucose/amino acid/calcium uptake and brush border membrane-associated enzymes in rat small intestines after the administration of embelin. J. Nutr. Sci. Vitaminol. (Tokyo) 36, 53–164.
Gupta, S., Sample, S. N., Kammar, U. (1991) Effects of embelin, a male antifertility agent, on absorptive and digestive functions of rat intestines. J. Ethnopharmacol. 33, 203–212.
Johri, R. K., Dhar, S. K., Puhma, S. G., Sharma, S. C., Kaul, J. L., Zutshi, U. (1990) Toxicity studies with potassium embelate, a new analgesic compound. Indian J. Exper. Biol. 28, 213–217.
Kokwaro, J. O. (1993) Medicinal plants of East Africa. Second Edition. Kenya Literature Bureau, Nairobi, Kenya.
Low, G., Rogers, L. G., Brunley, S. P. (1985) Visual deficits and retino-toxicity caused by naturally occurring anti-helmintics, embelia ribes and Hegenia abyssinica. Toxicol. Appl. Pharmacol. 81, 220–230.
Midiwo, J. O., Arot, L. M., Mbakaya, C. L. (1988) Distribution of benzoquinone pigments in Kenyan Myrcinaceae. Bull. Chem. Soc. Ethiophia 2, 83–85.
Mungai, N. N., Makawiti, D. W., Konji, N. V. (1997) Effects of different doses and routes of administration of embelin on plasma testosterone levels. Phytotherapy Research 11, 532–534.
Oduma, J. A., Wango, E. O., Oduor-Okelo, D., Makawiti, D. W. (1995) In vivo and in vitro effects of graded doses of the pesticide heptachlor on female sex steroid hormone production in rats. Comp. Biochem. Physiol. 2, 191–196.
Prakash, A. O. (1981) Anti-fertility investigations on embelin. Planta Medica 41, 259–266.
Prakash, A. O., Varshney, M. D. (1980) Effect of embelin, an anti-fertility agent of plant origin, on corpora lutea of suckling guinea pigs. J. Sci. Res. Plant Med. 1, 32–35.
Stoifer, R. L., Coesgen, J. I., Dizerega, G. S. (1981) Induction of defective corpus luteum function by administration of follicular fluid during the menstrual phase. Raven Press, New York, pp. 185–206.
Sufi, S. B., Donaldson, A., Jeffcoate, S. L. (1993) WHO Matched Reagent Programme Method Manual. 17ed.
Zuckerman, S., Parkes, A. S. (1976) Morphological and functional homologies of the male and female reproductive systems. Br. Med. J. 2, 864–865.
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Wango, E.O. Anti-Fertility Effects of Embelin in Female Sprague-Dawley Rats May Be Due to Suppression of Ovarian Function. BIOLOGIA FUTURA 56, 1–9 (2005). https://doi.org/10.1556/ABiol.56.2005.1-2.1
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DOI: https://doi.org/10.1556/ABiol.56.2005.1-2.1