Abstract
The action of a range of N terminally modified peptides structurally related to the nematode peptide PF1, SDPNFLRFamide, has been investigated using a dorsal muscle strip preparation from the chicken nematode, Ascaridia galli. Acetylcholine contracts this muscle preparation in a concentration-dependent manner when applied in the range 1–100 μM with an EC50 value of 9 μM. These contractions are reduced in the presence of PF1 and its analogues, with a threshold effect of PF1 of around 1 nM and an IC50 value of 470 nM against 10 μM acetylcholine. All the PF1 analogues tested were less potent than PF1 in reducing the acetylcholine contractions, indicating the importance of the N terminal amino acids in the action of PF1 in this preparation.
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Presented at the 10th ISIN Symposium on Invertebrate Neurobiology, July 5–9, 2003, Tihany, Hungary.
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Franks, C.J., Walker, R.J. & Holden-Dye, L. A Structure-Activity Study of the Neuropeptide PF1, SDPNFLRFamide, using the Dorsal Body Wall Muscle of the Chicken Nematode, Ascaridia Galli. BIOLOGIA FUTURA 55, 343–351 (2004). https://doi.org/10.1556/ABiol.55.2004.1-4.40
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DOI: https://doi.org/10.1556/ABiol.55.2004.1-4.40