Abstract
The proper division of cells is essential for the production of viable daughter cells. In plants and fungi, the dividing cell produces a cross-wall or septum that bisects the cytoplasm. For separation of the daughter cells, the septum has to be cleaved. To study the regulation of this process, we isolated mutants defective in septum cleavage. The mutants showed highly pleiotropic phenotypes and defined 17 novel genes. The deduced amino acid sequences of the products of the cloned genes exhibited homologies to various transcription regulators of other organisms. The homologies and the pleiotropic effects of the mutations on sexual development, stress response, mitotic stability, septum initiation and septum placement indicated that these genes affect cell separation indirectly, through multifunctional regulatory modules.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Belotserkovskaya, R., Sterner, D. E., Deng, M., Sayre, M. H., Lieberman, P. M., Berger, S. L. (2000) Inhibition of TATA-binding function by SAGA subunits Spt3 and Spt8 at Gcn4-activated promoters. Mol. Cell. Biol. 20, 634–647.
Bostock, C. J. (1970) DNA synthesis in the fission yeast Schizosaccharomycespombe. Exp. Cell Res. 60, 16–26.
Chang, M., Jaehning, J. A. (1997) A multiplicity of mediators: alternative forms of transcription complexes communicate with transcriptional regulators. Nucleic Acids Res. 25, 4861–4865.
Chant, J., Pringle, J. R. (1995) Patterns of bud-site selection in the yeast Saccharomyces cerevisiae. J. Cell Biol. 129, 751–765.
Fan, H.-Y., Klein, H. L. (1994) Characterization of mutations that suppress the temperature-sensitive growth of the hpr1Δ mutant of Saccharomyces cerevisiae. Genetics 137, 945–956.
Fankhauser, C., Reymond, A., Cerutti, L., Utzig, S., Hofmann, K., Simanis, V. (1995) The S. pombe cdc15 gene is a key element in the reorganisation of F-actin at mitosis. Cell 82, 435–444.
Grallert, A., Grallert, B., Ribár, B., Sipiczki, M. (1998) Coordination of initiation of nuclear division and initiation of cell division in Schizosaccharomyces pombe: Genetic interactions of mutations. J. Bacteriol 180, 892–900.
Grallert, A., Grallert, B., Zilahi, E., Szilágyi, Z., Sipiczki, M. (1999) Eleven novel sep genes of Schizosaccharomyces pombe required for efficient cell separation and sexual differentiation. Yeast 15, 669–686.
Grallert, A., Miklós, I., Sipiczki, M. (1997) Division site selection, cell separation and formation of anucleate minicells in Schizosaccharomyces pombe mutants resistant to cell wall lytic enzymes. Protoplasma 198, 218–229.
Grallert, A., Sipiczki, M. (1999) Identification of novel sep genes of Schizosaccharomyces pombe. Cancer and Cell Cycle. ISREC Conference. Lausanne (Switzerland). Abstract Book PA-87.
Grant, P. A., Berger, S. L. (1999) Histone acetyltransferase complexes. Cell Developmental Biol. 10, 169–177.
Hales, K. G., Bi, E., Wu, J-Q., Adam, J. C., Yu, I.-C, Pringle, J. R. (1999) Cytokinesis: an emerging unified theory for eukaryotes? Curr Opin. Cell. Biol 11, 717–725.
Hromas, R., Costa, R. (1995) The hepatocyte nuclear factor 3/fork head transcription regulatory family in development, inflammation and neoplasia. Crit. Rev Oncol. Hematol. 20, 129–140.
Johnson, B. F., Calleja, G. B., Zuker, M., McDonald, T. J. (1982) Cell division: key to cellular morphogenesis in the fission yeast Schizosaccharomyces pombe. Int. Rev. Cytol. 75, 167–208.
Kim, Y. J., Bjorklund, S., Li, Y., Sayre, M. H., Kornberg, R. D. (1994) A multiprotein mediator of transcription activation and its interaction with the C-terminal repeat domain of RNA polymerase II. Cell 77, 599–608.
Le Goff, X., Utzig, S., Simanis, V. (1999) Controlling septation in fission yeast: finding the middle, and timing it right. Curr. Genet. 35, 571–584.
Lee, Y C., Min, S., Gim, B. S., Kim, Y J. (1997) A transcriptional mediator protein that is required for activation of many RNA polymerase II promoters is conserved from yeast to humans. Mol. Cell Biol. 17, 4622–4632.
Marks, J., Hyams, J. S. (1985) Localization of F-actin through the cell division cycle of S pombe. Eur J. Cell Biol 39, 27–32.
McCollum, D., Balasubramanian, M. K., Pelcher, L. E., Hemmingsen, S. M., Gould, K. L. (1995) Schizosaccharomyces pombe cdc4+ gene encodes a novel EF-hand protein for cytokinesis. J. Cell Biol. 130, 651–660.
Miklös, I., Sipiczki, M. (1999) Analysis of a Schizosaccharomyces pombe cytokinesis mutant strain defective in the spl1 gene. Curr. Gen. 35, 439.
Murray, L. E., Rowley, N., Dawes, I. W., Johnston, G. C., Singer, R. A. (1998) Ayeast glutamine tRNA signals nitrogen status for regulation of dimorphic growth and sporulation. Proc. Natl. Acad. Sci. USA 95, 8619–8624.
Myers, L. C., Gustavson, C. M., Bushnell, D. A., Lui, M., Erdjument-Bromage, H., Tempst, P., Kornberg, R. D. (1998) The med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain. Genes Development 12, 45–54.
Ribar, B, Banrevi, A., Sipiczki, M. (1997) sep1 +encodes a transcription-factor homologue of the HNF-3/fork-head DNA-binding-domain family in Schizosaccharomyces pombe. Gene 202, 1–5.
Russel, P. R., Nurse, P. (1986) cdc25+ functions as an inducer of mitotic control of fission yeast. Cell 45, 145–153.
Russel, P. R., Nurse, P. (1987) Negative regulation of mitosis by wee1+, a gene encoding a protein kinase homolog. Cell 49, 559–567.
Sipiczki, M. (1988) The role of sterility genes (ste and aff) in the initiation of sexual development in Schizosaccharomyces pombe. Molec. Gen. Genet. 213, 529–534.
Sipiczki, M. (2000) Where does fission yeast sit on the tree of life? Genome Biology 1, 10111–10114.
Sipiczki, M., Bozsik, A. (2000) The use of morphomutants to investigate septum formation and cell separation in Schizosaccharomyces pombe. Arch. Microbiol. (in press).
Sipiczki, M., Grallert, B., Miklós, I. (1993) Mycelial and syncytial growth in Schizosaccharomyces pombe induced by novel septation mutations. J. Cell. Sci. 104, 485–493.
Sipiczki, M., Grallert, A., Miklös, I., Zilahi. E., Bozsik, A., Szilágyi, Z. (1999) Genetics, physiology and cytology of yeast-mycelial dimorphism in fission yeasts. Acta Microbiol. Immunol. Hung. 46, 297–302.
Sipiczki, M., Yamaguchi, M., Grallert, A., Takeo, K., Zilahi, E, Bozsik, A., Miklós, I. (2000) Role of cell shape in determination of the division plane in Schizosaccharomyces pombe: Random orientation of septa in spherical cells. J. Bacteriol. 182, 1693–1701.
Szilágyi, Z., Grallert, A., Zilahi, E., Sipiczki, M. (2000) The Schizosaccharomyces pombe sep10 gene encodes a conservative protein that presumably plays a role in transcription of a subset of genes. First Joint Meeting of the Slovenian Society for Microbiology and the Hungarian Society for Microbiology. Keszthely, Hungary. Programme and Abstracts p. 67.
Zilahi, E., Miklós, I., Sipiczki, M. (2000) The Schizosaccharomyces pombe sep15+ gene encodes a protein homologous to the Med8 subunit of the Saccharomyces cerevisiae transcriptional mediator complex. Curr Genet. (in press).
Zilahi, E., Salimova, E., Simanis, V., Sipiczki, M. (2000) The S pombe sep1 gene encodes a nuclear protein that influences the expression of the cdc15 gene. FEBS Letters (in press).
Author information
Authors and Affiliations
Corresponding author
Additional information
Dedicated to Professor Lajos Ferenczy on the occasion of his 70th birthday.
Rights and permissions
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
About this article
Cite this article
Sipiczki, M., Grallert, Á., Zilahi, E. et al. Multifunctional Cytokinesis Genes in Schizosaccharomyces pombe. BIOLOGIA FUTURA 52, 315–323 (2001). https://doi.org/10.1556/ABiol.52.2001.2-3.16
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1556/ABiol.52.2001.2-3.16