Fusarium head blight (FHB, caused by Fusarium graminearum) is a severe disease which threatens the yield and quality of harvested grain products, and hence causes major economic losses in wheat (Triticum spp.) and other small grain cereals. One of the infection routes for the pathogen is believed to be through the extruded anther. In order to study the role of the anther on FHB infection, FHB resistance was measured among wheat (Triticum aestivum L.) cultivars with non-extruded infertile anthers in cytoplasmic male sterile (CMS) lines and with extruded fertile anthers in their alloplasmic maintainer lines, and in an unrelated restorer line. We artificially inoculated the lines with F. graminearum (isolate NE 90) at 1.0 × 105 or 2.0 × 105 spores mL−1 for 72 or 96 hours. The results indicated that CMS cultivars were significantly more resistant than the maintainer and restorer cultivars. As the CMS lines differed from the maintainer lines by cytoplasm, anther health, anther extrusion, and seed set, additional experiments are needed to determine the role of each factor in infection. However, this research indicates that fertile anthers most likely are an important part of the infection pathway and of susceptibility to FHB.
Audenaert, K., Van Broeck, R., Bekaert, B., De Witte, F., Heremans, B., Messens, B., Höfet, M., Haesaert, G. 2009. Fusarium head blight (FHB) in Flanders: population diversity, inter-species associations and DON contamination in commercial winter wheat varieties. Eur. J. Plant Pathol. 125:445–458.
Brennan, J.M., Leonard, G., Fagan, B., Cooke, B. M., Ritient, A., Ferracane, R. 2007. Comparison of commercial European wheat cultivars to Fusarium infection of head and seedling tissue. Plant Pathol. 56:55–64.
Brown-Guedira, G., Griffey, C., Kolb, F., Mckendry, A., Murphy, J.P., Van Sanford, D. 2008. Breeding FHBresistant soft winter wheat: progress and prospects. In: Proc. 3rd Int. FHB Symposium Szeged, Hungary. Cereal Res. Commun., Suppl. B 36:31–35.
Cowger, C., Arrellano, C. 2010. Plump kernels with high deoxynivalenol linked to late Gibberella zeae infection and marginal disease conditions in winter wheat. Phytopathol. 100:719–728.
Cuthbert, P.A., Somers, D.J., Cloutier, S., Babel, A.B. 2006. Fine mapping Fhb1, a major gene controlling fusarium head blight resistance in bread wheat (Triticum aestivum L.). Theor. Appl. Genet. 112:1465–1472.
Dai, S.J. 2012. Cause analysis and countermeasures for FHB epidemic of wheat in Huaibei Area in 2012. J. Anhui Agri. Sci. 17:557–558. (in Chinese)
Doohan, F.M., Brennan, J., Cooke, B.M. 2003. Influence of climatic factors on Fusarium species pathogenic to cereal. Eur. J. Plant Pathol. 109:755–768.
Gomez, A.K., Gomez, A.A. 1984. Statistical Procedures for Agricultural Research. Second Ed. John Wiley & Sons. New York, NY, USA.
Groth, J., Tamburic-Ilincic, L., Schaafsma, A., Brule-Babel, A., Hartl, L. 2011. FHB resistance of winter wheat from Canada and Europe estimated across multi-environments after inoculation with two deoxynivalenol producing fusarium species. Cereal Res. Commun. 39:189–199.
Hernandez Nopsa, J.F., Baenziger, P. S., Eskridge, K.M., Peiris, K.H.S., Dowell, F.E., Harris, S.D., Wegulo, S.N. 2012. Differential accumulation of deoxynivalenol in two winter wheat cultivars varying in FHB phenotype response under field conditions. Can. J. Plant Pathol. 3:380–389.
Hernandez Nopsa, J.F., Wegulo, S.N., Panthi, A., Hallen-Adams, H.E., Harris, S.D., Baenziger, P.S. 2014. Characterization of Nebraska isolates of Fusarium graminearum causing head blight of wheat. Crop Sci. 54:310–317.
Jones, R.K., Mirocha, C.J. 1999. Quality parameters in small grains from Minnesota affected by fusarium head blight. Plant Dis. 83:506–511.
Kang, Z.S., Buchenauer, H. 2000. Cytology and ultrastructure of the infection of wheat spikes by Fusarium culmorum. Mycol. Res. 104:1083–1093.
Kubo, K., Kawada, N., Fujita, M., Hatta, K., Oda, S., Nakajima, T. 2010. Effect of cleistogamy on Fusarium head blight resistance in wheat. Breeding Sci. 60:405–411.
Kubo, K., Fujital, M., Kawadal, N., Nakajima, T., Nakamura K., Maejima, H., Ushiyama, T., Hatta, K., Matsunaka, H. 2013. Minor differences in anther extrusion affect resistance to Fusarium head blight in wheat. J. Phytopathol. 161:308–314.
Liu, Q.X., Lillemo, M., Skinnes, H., He, X.Y., Shi, J.R., Ji, F., Dong, Y.H., Bjørnstad, A. 2013. Anther extrusion and plant height are associated with Type I resistance to Fusarium head blight in bread wheat line ‘Shanghai-3/Catbird’. Theor. Appl. Genet. 126:317–334.
Matsui, K., Yoshida, M., Ban, T., Komatsuda, T., Kawada, N. 2002. Role of male-sterile cytoplasm in resistance to barley yellow mosaic virus and Fusarium head blight in barley. Plant Breed. 121:237–240.
McMullen, M., Jone, R., Gallenberg, G. 1997. Scab of wheat and barley: a re-emerging disease of devastating impact. Plant Dis. 81:1340–1348.
Miller, S.S., Chabot, D.M.P., Ouellet, T., Harris, L.J., Fedak, G. 2004. Use of a Fusarium graminearum strain transformed with green fluorescent protein to study infection in wheat (Triticum aestivum). Can. J. Plant Pathol. 26:453–463.
Nganje, W.E., Bangsung, D.A., Leistritz, F.L., Wilson, W.W., Tiapo, N.M. 2004. Regional economic impacts of fusarium head blight in wheat and barley. Rev. Agri. Eco. 26:332–347.
Parry, D.W., Jenkinson, P., Mcleod, L. 1995. Fusarium ear blight (Scab) in small-grain cereals: a review. Plant Pathol. 44:207–238.
Pugh, G.W., Johann, H., Dickson, J.G. 1933. Factors affecting infection of wheat heads by Gibberella saubinetti. J. Agri. Res. 46:771–797.
Schroeder, H.W. 1955. Factors affecting resistance of wheat to scab caused by Gibberella zeae (Schw.) Petch. PhD thesis. University of Minnesota. St Paul, MN, USA.
Schroeder, H.W., Christensen, J.J. 1963. Factors affecting resistance of wheat to scab by Gibberella zeae. Phytopathol. 53:831–838.
Shaner, G. 2003. Epidemiology of Fusarium head blight of small grain cereals in North America. In: Leonard, K.J., Bushnell, W.R. (eds), Fusarium Head Blight of Wheat and Barley. Am. Phytopathol. Soc. St. Paul, MN, USA. pp. 84–119.
Skinnes, H., Semagn, K., Tarkegne, Y., Maroy, A. G., Bjornstad, A. 2010. The inheritance of anther extrusion in hexaploid wheat and its relationship to Fusarium head blight resistance and deoxynivalenol content. Plant Breed. 129:149–155.
Song, J., Hedgcoth, C. 1993. Influence of nuclear background on transcription of a chimeric gene (orf 256) and cox1 in fertile and cytoplasmic male sterile wheats. Genome 37:203–209.
Steel, R.G.D., Torrie, J.H., Dickey, D.A. 1997. Principles and Procedures of Statistics. A Biometrical Approach. Third Ed. McGraw-Hill. New York, NY, USA.
Strange, R.N., Smith, H. 1971. A fungal growth stimulant in anthers which predisposes wheat to attack by Fusarium graminearum. Physiol. Plant Pathol. 1:141–150.
Strange, R.N., Majer, J.R., Smith, H. 1974. The isolation and identification of choline and betaine as the two major components in anthers and wheat germ that stimulate Fusarium graminearum in vitro. Physiol. Plant Pathol. 4:277–290.
Tu, C. 1930. Physiologic specialization in Fusarium spp. causing head-blight of small grains. Minnesota Agricultural Experimental Station. Technical Bulletin 74:1–27.
Tomasovic, S., Miinar, R., Ikic, I., Katarina, J., Palaversic, B. 2007. Breeding wheat for resistance to Fusarium head blight. Cereal Res. Commun. 35:1209–1212.
Wilson, J.A., Ross, W.M. 1962a. Cross breeding in wheat (Triticum aestivum) hybrid seed set on a cytoplasmic male-sterile winter wheat composite subjected to cross pollination. Crop Sci. 2:415–417.
Wilson, J.A., Ross, W.M. 1962b. Male sterility interaction of the Triticum aestivum nucleus and Triticum timopheevi cytoplasm. Wheat Inf. Serv. 14:29–30.
Zhang, Z.H., Zhang, X.K., Sun, D.J., Wang, X.D., Fu, X.J. 2009. Distribution of vernalization and photoperiod genes in Shaanxi wheat cultivars. J. Triticeae Crops 3:401–408. (In Chinese with English abstract)
Communicated by Á. Mesterházy and X.F. Zhang
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Wang, M.Y., Baenziger, P.S., El-Basyoni, I.S. et al. Comparison of Fusarium Head Blight Resistance in Cytoplasmic Male Sterile, Maintainer and Restorer Lines in Winter Wheat. CEREAL RESEARCH COMMUNICATIONS 43, 374–383 (2015). https://doi.org/10.1556/0806.43.2015.006