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Allelic variation in candidate genes associated with wood properties of cultivated poplars (Populus)

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Abstract

Introduction

Since Populus has veritable value as timber, plywood, pulp, and paper, genomic research should create the sound basis for further breeding toward desirable wood quality attributes.

Materials and methods

In this study, we addressed the need for a research methodology that initially identifies and then characterize candidate genes encoding enzymes with wood property phenotypic traits, toward the aim of developing a genomics-based breeding technology.

Results

On 23 different poplar species/hybrid samples, we successfully amplified 55 primers designed on Populus trichocarpa L. Considering the number of polymorphic sites, out of 73,206 bp, 51 SNPs and 31 indel events were found. Non-synonymous single base mutations could be detected in number of 30, 21 out of 164 sequences were the number of minimum recombination events and 41 significant pairwise comparisons between loci could be detected.

Discussion and conclusion

Our results provide a roadmap for a future association genetic study between nucleotide diversity and precise evaluation of phenotype.

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Authors and Affiliations

  1. Department of Tree Breeding, National Agricultural Research and Innovation Centre, Forest Research Institute, Várkerulet 30/A, Sárvár, 9600, Hungary

    Zoltán Attila Köbölkuti, Klára Cseke, Attila Benke & Attila Borovics

  2. Simonyi Karoly Faculty of Engineering, Wood Sciences and Applied Arts, University of Sopron, Bajcsy Zs. u. 4, 9400, Sopron, Hungary

    Mátyás Báder & Róbert Németh

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  1. Zoltán Attila Köbölkuti
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  2. Klára Cseke
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  3. Attila Benke
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  5. Attila Borovics
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  6. Róbert Németh
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Correspondence to Zoltán Attila Köbölkuti.

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Köbölkuti, Z.A., Cseke, K., Benke, A. et al. Allelic variation in candidate genes associated with wood properties of cultivated poplars (Populus). BIOLOGIA FUTURA 70, 286–294 (2019). https://doi.org/10.1556/019.70.2019.32

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