Abstract
Rht18, derived from Triticum durum (tetraploid) wheat, is classified as a gibberellic acid (GA)-responsive dwarfing gene. Prior to this study, the responses of Rht18 to exogenous GA on agronomic traits in hexaploid wheat were still unknown. The response of Rht18 to exogenous GA3 on coleoptile length, plant height, yield components and other agronomic traits were investigated using F4:5 and F5:6 hexaploid dwarf lines with Rht18 derived from two crosses between the tetraploid donor Icaro and tall Chinese winter wheat cultivars, Xifeng 20 and Jinmai 47. Applications of exogenous GA3 significantly increased coleoptile length in both lines and their tall parents. Plant height was significantly increased by 21.3 and 10.7% in the GA3-treated dwarf lines of Xifeng 20 and Jinmai 47, respectively. Compared to the untreated dwarf lines, the partitioning of dry matter to ears at anthesis was significantly decreased while the partitioning of dry matter to stems was significantly increased in the GA3-treated dwarf lines. There were no obvious changes in plant height and dry matter partitioning in the GA3-treated tall parents. Exogenous GA3 significantly decreased grain number spike−1 while it increased 1000-kernel weight in both the dwarf lines and tall parents. Thus, applications of exogenous GA3 restored plant height and other agronomic traits of Rht18 dwarf lines to the levels of the tall parents. This study indicated that Rht18 dwarf mutants are GA-deficient lines with impaired GA biosynthesis.
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Botwright, T.L., Rebetzke, G.J., Condon, A.G., Richards, R.A. 2005. Infuence of the gibberellin-sensitive Rht8 dwarfing gene on leaf epidermal cell dimensions and early vigour in wheat (Triticum aestivum L.). Ann. Bot. 95:631–639.
Brooking, I.R., Kirby, E.J.M. 1981. Interrelationships between stem and ear development in winter wheat: the effects of a Norin 10 dwarfing gene, Gai/Rht2. J. Agric. Sci. 97:373–381.
Chen, L., Hao, L., Condon, A.G., Hu, Y.-G. 2014. Exogenous GA3 application can compensate the morphogenetic effects of the GA-responsive dwarfing gene Rht12 in bread wheat. PLoS ONE 9:e86431.
Colombo, N., Favret, E. 1996. The effect of gibberellic acid on male fertility in bread wheat. Euphytica 91:297–303.
Ellis, M.H., Rebetzke, G.J., Chandler, P., Bonnett, D., Spielmeyer, W., Richards, R.A. 2004. The effect of different height reducing genes on the early growth of wheat. Funct. Plant Biol. 31:583–589.
Fleet, C.M., Sun, T.-P. 2005. A DELLAcate balance: the role of gibberellin in plant morphogenesis. Curr. Opin. Plant Biol. 8:77–85.
Hedden, P. 2003. The genes of the Green Revolution. Trends Genet. 19:5–9.
Keyes, G.J., Paolillo, D.J., Sorrells, M.E. 1989. The effects of dwarfing genes Rht1 and Rht2 on cellular dimensions and rate of leaf elongation in wheat. Ann. Bot. 64:683–690.
Konzak, C.F. 1987. Mutations and mutation breeding. In: Heyne, E.C. (ed.), Wheat and Wheat Improvement. Am. Soc. Agron. Madison, WI, USA. pp. 428–443.
Konzak, C.F. 1988. Genetic analysis, genetic improvement and evaluation of induced semi-dwarf mutants in wheat. In: Semidwarf Cereal Mutants and Their Use in Cross-Breeding III. Research Coordination Meeting, December, 16–20, 1985, International Atomic Energy Agency. Vienna, Austria. pp. 77–94.
Magome, H., Yamaguchi, S., Hanada, A., Kamiya, Y., Oda, K. 2004. Dwarf and delayed-fowering 1. A novel Arabidopsis mutant deficient in gibberellin biosynthesis because of over expression of a putative AP2 transcription factor. Plant J. 37:720–729.
Peng, J., Richards, D.E., Hartley, N.M., Murphy, G.P., Devos, K.M., Flintham, J.E., Beales, J., Fish, L.J., Worland, A.J., Pelica, F. 1999. ‘Green Revolution’ genes encode mutant gibberellin response modulators. Nature 400:256–261.
Rebetzke, G.J., Richards, R.A., Fischer, V.M., Mickelson, B.J. 1999. Breeding long coleoptile, reduced height wheats. Euphytica 106:159–168.
Rebetzke, G.J., Richards, R.A., Sirault, X.R.R., Morrison, A.D. 2004. Genetic analysis of coleoptile length and diameter in wheat. Aust. J. Agric. Res. 55:733–743.
Rebetzke, G.J., Ellis, M.H., Bonnett, D.G., Mickelson, B., Condon, A.G., Richards, R.A. 2012. Height reduction and agronomic performance for selected gibberellin-responsive dwarfing genes in bread wheat (Triticum aestivum L.). Field Crops Res. 126:87–96.
Richards, D.E., King, K.E., Ait-Ali, T., Harberd, N.P. 2001. How gibberellin regulates plant growth and development: a molecular genetic analysis of gibberellin signaling. Annu. Rev. Plant Physiol. Plant Mol. Biol. 52:67–88.
Sasaki, A., Ashikari, M., Ueguchi-Tanaka, M., Itoh, H., Nishimura, A., Swapan, D., Ishiyama, K., Saito, T., Kobayashi, M., Khush, G. 2002. Green revolution: a mutant gibberellin-synthesis gene in rice. Nature 416:701–702.
Spielmeyer, W., Ellis, M.H., Chandler, P.M. 2002. Semidwarf (sd-1), “Green Revolution” rice, contains a defective gibberellin 20-oxidase gene. Proc. Natl Acad. Sci. USA 99:9043–9048.
Wang, Z., Cao, W., Dai, T., Zhou, Q. 2001. Effects of exogenous hormones on floret development and grain set in wheat. Plant Growth Regul. 35:225–231.
Worland, A., Sayers, E., Börner, A. 1994. The genetics and breeding potential of Rht12, a dominant dwarfing gene in wheat. Plant Breed. 113:187–196.
Yamaguchi, S. 2008. Gibberellin metabolism and its regulation. Annu. Rev. Plant Biol. 59:225–251.
Yang, Z., Zheng, J., Liu, C., Wang, Y., Condon, A.G., Chen, Y., Hu, Y.-G. 2015. Effects of the GA-responsive dwarfing gene Rht18 from tetraploid wheat on agronomic traits of common wheat. Field Crops Res. 183:92–101.
Zadoks, J.C., Chang, T.T., Konzak, C.F. 1974. A decimal code for the growth stages of cereals. Weed Res. 14:415–421.
Acknowledgements
This work was financially supported by the sub-project of the 863 Program (2013AA102902) of the Ministry of Science and Technology; the key project of Chinese Universities Scientific Fund, Northwest A&F University (ZD2012002); and Australian Centre for International Agricultural Research (ACIAR) Project (CIM/2005/111). We are grateful to Dr. Anthony Condon of CSIRO Agriculture Flagship for providing cv. Icaro and pertinent suggestions for the experiments. Thanks also to the anonymous reviewers for their great help on revising the manuscript.
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Yang, Z.Y., Liu, C.Y., Du, Y.Y. et al. Dwarfing Gene Rht18 from Tetraploid Wheat Responds to Exogenous GA3 in Hexaploid Wheat. CEREAL RESEARCH COMMUNICATIONS 45, 23–34 (2017). https://doi.org/10.1556/0806.44.2016.050
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DOI: https://doi.org/10.1556/0806.44.2016.050