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Radical-scavenging activity of glutathione, chitin derivatives and their combination

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Abstract

Since chitosan and its amino-, cinnamo- or cinnamo-amino- derivatives are acid-soluble, the effect of acetic acid on hyaluronan (HA) macromolecules degraded by Cu(II) ions and ascorbate was examined to produce reactive oxygen species (ROS). Further, the effects of glutathione (GSH), chitosan and its derivatives, added individually or in combination, on the quenching of ROS and ABTS˙+ cation radical were examined using rotational viscometry and ABTS assay, respectively. The results of the rotational viscometry indicated a rapid degradation of HA by ROS after the addition of acetic acid. Chitosan and its derivatives moderately decreased the rate of HA degradation, while GSH decreased the rate of HA degradation more significantly. Moreover, GSH enhanced the protection of HA macromolecules against their degradation in the presence of chitosan or its derivatives. The results of the ABTS assay confirmed the results of the rotational viscometry. The GSH in the combination with chitosan and its derivatives reduced ABTS˙+ more intensively than when added individually.

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Authors and Affiliations

  1. Laboratory of Bioorganic Chemistry of Drugs, Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Dúbravská cesta 9, 84104, Bratislava, Slovakia

    Katarína Valachová & Ladislav Šoltés

  2. Polymer Materials Research Department, Advanced Technologies and New Materials Research Institute (ATNMRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, 21934, Alexandria, Egypt

    Tamer Mahmoud Tamer

  3. Chemistry Department, Faculty of Science, University of Jeddah, Osfan, P. O. Box: 80203, 21589, Jeddah, Saudi Arabia

    Mohamed Mohy Eldin

Authors
  1. Katarína Valachová
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  2. Tamer Mahmoud Tamer
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  3. Mohamed Mohy Eldin
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  4. Ladislav Šoltés
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Correspondence to Katarína Valachová.

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Valachová, K., Tamer, T.M., Eldin, M.M. et al. Radical-scavenging activity of glutathione, chitin derivatives and their combination. Chem. Pap. 70, 820–827 (2016). https://doi.org/10.1515/chempap-2016-0011

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  • DOI: https://doi.org/10.1515/chempap-2016-0011

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