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Detection of hydrogen peroxide and glucose by enzyme product precipitation on sensor surface

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Abstract

Biocatalysed precipitation of an insoluble product accumulated on the enzyme-modified electrode surface was applied as the amplification path for low concentration sensing of hydrogen peroxide and glucose. Sensitive electrochemical and quartz-crystal microbalance (QCM) biosensors based on biocatalytic precipitation were developed. A horseradish peroxidase (HRP) monolayer-modified electrode was used to sense H2O2 via the oxidation of 4-chloro-1-naphthol (4CN) forming insoluble benzo-4-chlorocyclohexadienone. Additionally, the bienzyme system employed glucose oxidase (GOx) linked to HRP/4CN. The amount of the precipitate assembled on the sensing surface corresponded to the concentration of analytes and to the length of the incubation interval. The precipitated deposits were followed as a change of impedance using cyclic voltammetry (CV), mass change was determined continuously using a microgravimetric quartz-crystal microbalance, and optical microscopy enabled the visualisation of the precipitate. Regeneration of the enzyme-modified electrode was performed using cathodic reduction of the insoluble product. Thus, a simple biosensor for multiple analyses with low detection limits and of low cost can be developed.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic

    Tomáš Juřík & Petr Skládal

  2. Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic

    Tomáš Juřík & Petr Skládal

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  1. Tomáš Juřík
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  2. Petr Skládal
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Correspondence to Petr Skládal.

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Juřík, T., Skládal, P. Detection of hydrogen peroxide and glucose by enzyme product precipitation on sensor surface. Chem. Pap. 69, 167–175 (2015). https://doi.org/10.1515/chempap-2015-0003

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