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Detection of the antibacterial effect of Chaetomium cochliodes Palliser CCM F-232 based on agar plugs and unprocessed fungal substances from cultivation media

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Abstract

There is an increasing demand for novel antibiotics that are highly effective, but simultaneously reveal minimal side effects. Currently, major sources of antibiotics are found among bacterial genus Streptomyces and among fungal genera Penicillium and Aspergillus. Chaetomium represents the largest genus of the family Chaetomiaceae actually containing more than 100 described species. This rather complex genus has been reported as a rich source of secondary metabolite products with important biological activities, such as enzyme-inhibitory, antitumour, cytotoxic, antimalarial and also antibiotic effects. One of them, chaetomin, a part of Chaetomium cochliodes antimicrobial equipment was discovered already in the early period of antibiotics discovery, but later not investigated in sufficient detail. In this contribution we have studied Chaetomium cochliodes Palliser CCM F-232 for its possible antibacterial properties. For comparisons, a closely related fungus Chaetomium globosum was also employed. Antibacterial properties of Chaetomium sp. were tested on natural bacterial isolates of Staphylococcus aureus and Bacillus subtilis. We performed several antimicrobial tests with a correlating output. Antibacterial effects of these filamentous fungi were demonstrated either by the presence of susceptibility zones on solid agar media in the case of S. aureus or by growth inhibition in liquid broth in the case of Staphylococcus aureus and Bacillus subtilis.

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Abbreviations

A600:

absorbance measured at a wavelength of 600 nm

CD agar or broth:

Czapek Dox agar or broth with 1% corn extract powder

IM:

intermediate

LB:

Luria-Bertani

MPG:

agar or broth, malt peptone glucose agar or broth

PME:

agar or broth, potato malt extract agar or broth with stripes of filter paper

RT:

room temperature 0.45 μmCco/Cgl MPG/PME/CD, filtrates through 0.45 μm membrane obtained from C. cochliodes or C. globosum cultivated in MPG, PME or CD broth

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Acknowledgements

Our research was supported by the Slovak Research and Development Agency with grant APVV-14-0375 and by the Slovak Grant Agency VEGA with grant 2/0021/14.

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Authors and Affiliations

  1. Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, SK-84551, Bratislava, Slovakia

    Katarína Chovanová & Marcel Zámocký

  2. Division of Biochemistry, Department of Chemistry, BOKU-University of Natural Resources & Life Sciences, Muthgasse 18, 1190, Vienna, Austria

    Marcel Zámocký

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  1. Katarína Chovanová
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  2. Marcel Zámocký
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Correspondence to Katarína Chovanová.

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Chovanová, K., Zámocký, M. Detection of the antibacterial effect of Chaetomium cochliodes Palliser CCM F-232 based on agar plugs and unprocessed fungal substances from cultivation media. Biologia 71, 1204–1211 (2016). https://doi.org/10.1515/biolog-2016-0153

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