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Identification and molecular characterization of one novel 1SPl-encoded s-type low molecular weight glutenin B-subunit from 1Sl(1B) substitution line of wheat variety Chinese Spring (Triticum aestivum)

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Abstract

Aegilops longissima (2n = 2x = 14, SlSl), has extensive allelic variations of glutenin subunits that are considered as useful gene sources for wheat quality improvement. The Chinese Spring 1Sl(1B) chromosome substitution line CS-1Sl(1B) showed superior dough properties and breadmaking quality due to the introgression of novel glutenin subunits encoded by 1Sl genome. In this study, we identified one novel 1Sl-encoded low molecular weight glutenin B-subunit 1SlLMW-s from CS-1Sl(1B). Its complete encoding sequences were isolated and designated as 1SlLMW-s with 960 bp encoding 318 amino acid residues. Molecular characterization demonstrated that the deduced 1SlLMW-s subunit had a rather large and regular repeated sequence domain, including a high proportion of glutamine residues (about 44%) in the repeats (consensus sequence PPFSQQQQ). A total of 31 SNPs were detected at different positions of encoding sequences. The secondary structure prediction revealed higher β-strand and α-helix content present in 1SlLMW-s. Phylogenetic tree revealed that 1SlLMW-s had close evolutionary relationship with other s-type low molecular weight glutenin subunit (LMW-GSs) genes from different Triticum and Aegilops genomes, which was divergent from LMW-s type gene subfamily at 3.92–5.23 million years ago (MYA). LMW-GSs play a key role in improving breadmaking quality. Abundant expression and specific structural features could contribute to superior gluten quality, including larger and more regular repeated domain, higher proportion of glutamine residues and higher β-strand and α-helix content. This could facilitate the formation of stronger dough structure and superior breadmaking quality. Our work demonstrated that Sl genome had potential glutenin gene resources, and particularly 1SlLMW-s gene could be useful for wheat quality improvement.

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Abbreviations

AS-PCR:

allelic specific polymerase chain reaction

LMW-GSs:

low molecular weight glutenin subunit

MYA:

million years ago

HMW-GSs:

high molecular weight glutenin subunits

SDS-PAGE:

sodium dodecyl sulfate polyacrylamide gel electrophoresis

CS:

Chinese Spring

HPCE:

High-performance capillary electrophoresis

HPMC:

hydroxypropyl methylcellulose

2-DE:

two-dimensional electrophoresis

MALDI-TOF/TOF:

matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry

SNPs:

single-nucleotide polymorphisms

InDels:

insertions/deletions.

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Authors and Affiliations

  1. Collegeof Life Science, Capital Normal University, Beijing, 100048, China

    Xiong Deng, Shun-li Wang, Shou-min Zhen & Yue-ming Yan

  2. Hubei Collaborative Innovation Center for Grain Industry, Yangtze University, Jingzhou, 434025, China

    Wen-ying Zhang & Yue-ming Yan

  3. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China

    Shun-li Wang

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  1. Xiong Deng
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Identification and molecular characterization of one novel 1SPl-encoded s-type low molecular weight glutenin B-subunit from 1Sl(1B) substitution line of wheat variety Chinese Spring (Triticum aestivum)

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Deng, X., Wang, Sl., Zhen, Sm. et al. Identification and molecular characterization of one novel 1SPl-encoded s-type low molecular weight glutenin B-subunit from 1Sl(1B) substitution line of wheat variety Chinese Spring (Triticum aestivum). Biologia 71, 1212–1222 (2016). https://doi.org/10.1515/biolog-2016-0147

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