Abstract
This study was aimed to explore antibiofilm agents from the hitherto underexplored Palk Bay seawater bacteria. The cell free culture supernatant of the isolate Marinomonas sp. showed profound antibiofilm activity against Vibrio parahaemolyticus ATCC 17802. The active principle responsible for antibiofilm activity was identified as indole-3-carboxaldehyde (ICA) after bioassay guided purification and gas chromatography-mass spectrometry analysis. Further, in vitro antibiofilm activity of ICA was confirmed through light microscopy, confocal imaging, scanning electron microscopy and biofilm disruption studies. In addition, ICA efficiently reduced the swarming motility of the pathogen and promoted the swimming ability. Furthermore, the control of biofilms and swarming efficiency by quorum sensing pathway of the pathogen was modulated by ICA, which was substantiated using real-time analysis for opaR, cpsA, and lafA genes. This study divulged the efficacy of ICA as an antibiofilm agent against V. parahaemolyticus in vitro.
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Abbreviations
- BIC:
-
biofilm inhibitory concentration
- CFCS:
-
cell-free culture supernatant
- CLSM:
-
confocal laser scanning microscopy
- DPA:
-
2,6-di-O-palmitoyl-L-ascorbic acid
- GC-MS:
-
gas chromatography mass spectrometry
- ICA:
-
indole-3-carboxaldehyde
- LB:
-
Luria Bertani
- LM:
-
light microscopy
- MIC:
-
minimal inhibitory concentration
- mLB:
-
marine Luria Bertani
- OD:
-
optical density
- QS:
-
quorum sensing
- SA:
-
stearic acid
- SEM:
-
scanning electron microscopy
- ZMB:
-
Zobell marine broth
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Acknowledgements
The authors thankfully acknowledge the Bioinformatics Infrastructure Facility funded by Department of Biotechnology, Government of India [Grant No. BT/BI/25/012/2012 (BIF)], the instrumentation facility provided by Department of Science and Technology, Government of India through PURSE [Grant No.SR/S9Z-23/2010/42 (G)] & FIST (Grant No.SR-FST/LSI-087/2008), and University Grants Commission, New Delhi, through SAP-DRS1 [Grant No.F.3-28/2011 (SAP-II)].
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Rajalaxmi, M., Amsa Devi, V. & Pandian, S.K. In vitro evaluation of indole-3-carboxaldehyde on Vibrio parahaemolyticus biofilms. Biologia 71, 247–255 (2016). https://doi.org/10.1515/biolog-2016-0043
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DOI: https://doi.org/10.1515/biolog-2016-0043