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Antimicrobial resistance and molecular characterisation of human campylobacters from Slovakia

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Abstract

This paper delivers molecular biological study of antimicrobial resistance in clinical isolates of Campylobacter spp. in Slovakia. During the year 2015 a total of 828 Campylobacter strains collected from five regions in Slovakia were examined for resistance to ciprofloxacin (CIP), tetracycline (TET), erythromycin (ERY) and ampicillin with sulbactam (SAM). The highest incidence of Campylobacter resistance was observed for CIP (64.8%), followed by TET (30.5%) and SAM (26.3%). All strains were susceptible to ERY. Twenty selected antibiotic-resistant strains were examined for the presence Campylobacter β-lactamase OXA-61, multidrug efflux pump cmeB and TET tet(O) genes by PCR. From twenty CIP-resistant strains, the cmeB gene was present in 12, while the chromosomal OXA-61 β-lactamase occurred in 11 strains from 18 SAM-resistant Campylobacters, and tet(O) was present in 14 strains from 18 TET-resistant ones investigated. All three genes together occurred within a single strain in five Campylobacter jejuni and one Campylobacter coli strains isolated from eastern Slovakia. Clonal relatedness of strains was detected by a MALDI biotyper, which revealed two clusters of C. jejuni and one cluster of C. coli. The results showed that the epidemiologically important strains with high fluoroquinolone resistance occurred among the Campylobacter strains. An important finding is represented also by the evidence of the OXA-61 β-lactamase in more than one half of investigated strains, because SAM is frequently used for therapy in children and pregnant women.

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Abbreviations

CIP:

ciprofloxacin

ERY:

erythromycin

SAM:

ampicillin with sulbactam

TET:

tetracycline

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Acknowledgements

We are grateful to all of the microbiologists and technicians of the HPL spol. s r.o. that collected the isolates for us. This work was supported by funding from the Slovak Researchand Development Agency (grant No. APVV-14-0274) and from ERDF No. 26220220065.

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Authors and Affiliations

  1. HPL s.r.o., Magnezitárska 2/C, SK-04013, Košice, Slovakia

    Juraj Hanzen & Jana Choková

  2. Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4, SK-04001, Košice, Slovakia

    Vladimír Kmeť

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  1. Juraj Hanzen
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  2. Jana Choková
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  3. Vladimír Kmeť
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Correspondence to Vladimír Kmeť.

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Hanzen, J., Choková, J. & Kmeť, V. Antimicrobial resistance and molecular characterisation of human campylobacters from Slovakia. Biologia 71, 117–120 (2016). https://doi.org/10.1515/biolog-2016-0029

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