Skip to main content
SpringerLink
Log in

Neurofilaments in thyrotrophs of hypothyroid rats: An immunohistochemical study

  • Basic Research
  • Published:
Endocrine Pathology Aims and scope Submit manuscript

Abstract

We examined by immunohistochemistry the expression of 200-kDa (NF-H) neurofilaments in pituitary thyrotrophs of rats, made hypothyroid by propylthiouracil (PTU) administration. Rats were sacrificed at 3, 7, 14, and 28 d of PTU administration as well as at 3, 7, and 14 d after interruption of a 14-d PTU treatment. The pituitaries were fixed, dehydrated, and embedded in paraffin. Consecutive sections were prepared for the immunohistochemical demonstration of thyroid-stimulating hormone and NF-H. NF-H were found in some thyrotrophs of the adenohypophysis of control rats. NF-H were expressed in thyroidectomy cells of hypothyroid rats. Discontinuation of PTU treatment led to recovery from cytologic alterations in the thyroidectomy cells expressing NF-H.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Riedered BM, Porchet R, Marrugg RA. Differential expression and modification of neurofilament triplet proteins during cat cerebellar development. J Comp Neurol 364:704–717, 1996.

    Article  Google Scholar 

  2. Ogawa A, Sugihara S, Hasegawa M, Sasaki A, Nakazato Y, Kawada T, Ishiuchi S, Tamura M. Intermediate filament expression in pituitary adenomas. Virchows Archiv B Cell Pathol 58:341–349, 1990.

    CAS  Google Scholar 

  3. Ogawa A, Sugihara S, Nakanishi Y, Suzuki S, Sasaki A, Hirato J, Nakazato Y. Intermediate filament expression in non-neoplastic pituitary cells. Virchows Archiv B Cell Pathol 58:331–340, 1990.

    Article  CAS  Google Scholar 

  4. Osborn M, Weber K. Biology of disease: tumor diagnosis by intermediate filament typing: a novel tool for surgical pathology. Lab Invest 48:372–394, 1983.

    PubMed  CAS  Google Scholar 

  5. Surks MI, DeFesi CR. Determination of the number of each cell type in the anterior pituitary of euthyroid and hypothyroid rats. Endocrinology 101:946–958, 1977.

    Article  PubMed  CAS  Google Scholar 

  6. Asa SL. Tumors of the pituitary gland. In: Atlas of tumor pathology. 3rd series, fascicle 22. Washington, DC: Armed Forces Institute of Pathology 194, 1998.

    Google Scholar 

  7. Beck-Peccoz P, Brucker-Davis F, Persani L, Smallridge RC, Weintraub BD. Thyrotropinsecreting pituitary tumors. Endocr Rev 17:610–638, 1996.

    Article  PubMed  CAS  Google Scholar 

  8. Horvath E, Lloyd RV, Kovacs K. Propylthiouracylinduced hypothyroidism results in reversible transdifferentiation of somatotrophs into thyroidectomy cell. Lab Invest 63:511–520, 1990.

    PubMed  CAS  Google Scholar 

  9. Hsu SM, Raine L, Fanger H. The use of antiavidin antibody and avidin-biotin-peroxidase complex in immunoperoxidase technics. Am J Clin Pathol 75:816, 1981.

    PubMed  CAS  Google Scholar 

  10. Woosley JT. Measuring cell proliferation. Arch Pathol Lab Med 115:555–557, 1991.

    PubMed  CAS  Google Scholar 

  11. Dubois PM, ElAmraoui A. Embryology of the pituitary gland. Trends Endocrinol Metab 6:1–6, 1995.

    Article  CAS  PubMed  Google Scholar 

  12. Bäck N, Tyynelä M, Portier MM, Virtanen I, Soinila S. Distribution of neurofilament proteins and peripherin in the rat pituitary gland. Neurosci Res 22:267–275, 1995.

    Article  PubMed  Google Scholar 

  13. Li M, Boyages SC. Expression of beta2-thyroid hormone receptor in euthyroid and hypothyroid rat pituitary gland: an in situ hybridization and immunocytochemical study. Brain Res 773:125–131, 1997.

    Article  PubMed  CAS  Google Scholar 

  14. Gravel C, Hawkes R. Thyroid hormone modulates the expression of a neurofilament antigen in the cerebellar cortex: premature induction and overexpression by basket cells in hypothyroidism and a critical period for the correction of hypothyroidism. Brain Res 422:327–335, 1987.

    Article  PubMed  CAS  Google Scholar 

  15. Ozawa H. Changing ultrastructure of thyrotrophs in the rat anterior pituitary after thyroidectomy as studied by immuno-electron microscopy and enzyme cytochemistry. Cell Tissue Res 263:405–412, 1990.

    Article  Google Scholar 

  16. Kurosumi K. Ultrastructural immunocytochemistry of the adenohypophysis in the rat: a review. J Electron Microsc Technol 19:42–56, 1991.

    Article  CAS  Google Scholar 

  17. Kiguchi Y. The process of development of thyroidectomy cells from the so-called thyrotrophs. Endocrinol Jpn 25:75–86, 1978.

    PubMed  CAS  Google Scholar 

  18. Ravindra R, Grosvenor CE. Involvement of cytoskeleton in polypeptide hormone secretion from the anterior pituitary lobe: a review. Mol Cell Endocrinol 71:165–176, 1990.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physiology and Pharmacology, Universidad Autónoma de Aguascalientes, Aguascalientes, México

    J. Luis Quintanar PhD

  2. Department of Microbiology, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, México

    Eva Salinas PhD

Authors
  1. Eva Salinas PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Luis Quintanar PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. Luis Quintanar PhD.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Salinas, E., Quintanar, J.L. Neurofilaments in thyrotrophs of hypothyroid rats: An immunohistochemical study. Endocr Pathol 11, 195–200 (2000). https://doi.org/10.1385/EP:11:2:195

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1385/EP:11:2:195

Key Words

Search

Navigation