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Regulation of GnRH I receptor gene expression by the GnRH agonist triptorelin, estradiol, and progesterone in the gonadotroph-derived cell line αT3-1

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Abstract

The secretion of luteinizing hormone (LH) and the GnRH receptor (GnRH-R) concentration are modulated by ovarian steroids and GnRH. To elucidate whether this regulation is due to alterations at the transcriptional level, we examined the GnRH I-R mRNA expression in the gonadotroph-derived cell line α T3-1 treated with different estradiol and progester-one paradigms and the GnRH I agonist triptorelin. αT3-1 cells were treated with different steroid paradigms: 1 nM estradiol or 100 nM progesterone for 48 h alone or in combination. Cells were exposed to 10 nM or 100 pM triptorelin for 30 min, 3 h, 9 h, or, in pulsatile way, with a 5-min pulse per hour. The GnRH I-R mRNA was determined by Northern blot analysis. GnRH I-R mRNA from cells treated with continuous triptorelin decreased in a time- and concentration-dependent manner. Pulsatile triptorelin increased GnRH I-R gene expression. Progesterone alone further enhanced this effect, whereas estradiol and its combination with progesterone diminished it. Continuous combined treatment with estradiol and progesterone lead to a significant decrease of GnRH I-R mRNA by 30% and by 35% for estradiol alone. The addition of 10 nM triptorelin for 30 min or 3 h could not influence that steroideffect. In conclusion, estradiol and progesterone exclusively decreased GnRH I-R mRNA in αT3-1 cells no matter whether they are treated additionally with the GnRH I agonist triptorelin. The enhanced sensitivity of gonadotrophs and GnRH I-R upregulation by estradiel is not due to increased GnRH I gene expression because GnRH I-R mRNA is downregulated by estradiol and progesterone. Other pathways of the GnRH I-R signal transduction might be involved.

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Authors and Affiliations

  1. Department of Obstetrics and Gynaecology, Medical University Luebeck, Ratzeburger Allee 160, 23538, Luebeck, Germany

    J. M. Weiss PD, S. Polack & K. Diedrich

  2. Department of Obstetrics and Gynecology, Caritas St. Josef Hospital, University of Regensburg, Postfach 10 06 45, D-93006, Regensburg, Germany

    O. Treeck & O. Ortmann

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  1. J. M. Weiss PD
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  2. S. Polack
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  4. K. Diedrich
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  5. O. Ortmann
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Correspondence to J. M. Weiss PD.

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Weiss, J.M., Polack, S., Treeck, O. et al. Regulation of GnRH I receptor gene expression by the GnRH agonist triptorelin, estradiol, and progesterone in the gonadotroph-derived cell line αT3-1. Endocr 30, 139–144 (2006). https://doi.org/10.1385/ENDO:30:1:139

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  • DOI: https://doi.org/10.1385/ENDO:30:1:139

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