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Mitogenic responsiveness of caprine mammary epithelial cells to endocrine and cytokine factors

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Abstract

Mammary cell mitogenic responsiveness was evaluated with an established nontransformed caprine mammary epithelial cell line (CMEC). As expected, the cells responded to insulin-like growth factor 1 (IGF-1), insulin, and hydrocortisone with an increased number of cells after 3 and 5 d in culture. In combination, insulin and hydrocortisone augmented each other. A proliferation response was also observed for transforming growth factor-α (TGF-α), and epidermal growth factor (EGF), but not for fibroblast growth factor a (FGFa), FGFb, IGF-2, bovine somatotropin, or prolactin. Comparison of mitogenic potential for these growth factors with cells grown on plastic substratum indicated that hydrocortisone was most potent, followed by TGF-α in inducing a proliferative response measured by 3H-thymidine incorporation assay. Hydrocortisome augmented proliferation by 149% and TGF-α stimulated proliferation by 126% relative to the media control (p<0.01). EGF, which binds to the same receptor as TGF-α in other species, induced a modest 35% increase in proliferation. Comparison of culture conditions with plastic, fibronectin, and type I collagen suggests that extracellular matrix/stroma influences the magnitude and effective concentration for cytokine-mediated growth response. Studies on responsiveness to ovarian steroids estradiol 17-β (E2) and progesterone (P4) showed a modest proliferation response to E2 only in combination with triiodio-l-thyronine (T3), and no response to P4 or T3 either alone or in combination when grown on plastic.

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Authors and Affiliations

  1. Department of Pathobiology, University of Connecticut, U-89, 06269-3089, Storrs, CT

    Alexander G. Pantschenko & T. J. Yang

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  1. Alexander G. Pantschenko
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  2. T. J. Yang
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Correspondence to T. J. Yang.

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Pantschenko, A.G., Yang, T.J. Mitogenic responsiveness of caprine mammary epithelial cells to endocrine and cytokine factors. Endocr 10, 123–130 (1999). https://doi.org/10.1385/ENDO:10:2:123

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  • DOI: https://doi.org/10.1385/ENDO:10:2:123

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