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Biophysical studies on the differentiation of human CD14+ monocytes into dendritic cells

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Abstract

Dendritic cells (DCs), which are the most efficient antigen-presenting cells (APCs) currently known, can be derived from CD14+ monocytes (DC predecessor cells) in vitro. Immature DCs actively take up antigens and pathogens, generate major histocompatability complex-peptide complexes, and migrate from the sites of antigen acquisition to secondary lymphoid organs to become mature dendritic cells that interact with and stimulate T-lymphocytes. During this process, the cells must undergo deformation to translocate through several barriers, including the basement membrane and interstitial connective tissue in the blood vessel wall. To further understand the mechanisms of the activation of immunological responses and the migration from peripheral tissue to secondary lymphoid organs, we have applied biophysical and microrheological methods to study the development processes of DCs in vitro. The results showed that membrane fluidity, osmotic fragility, membrane viscoelastic properties, infrared spectroscopy, and cytoskeleton organization of DCs exhibit significant differences in different developmental stages.

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Authors and Affiliations

  1. Department of Biophysics Health Science Center, Peking University, Beijing, People’s Republic of China

    Zhu Zeng, Xiao Liu, Yuhui Jiang, Jun Guo, Weijuan Yao & Zongyao Wen

  2. Department of Medical Physics, Health Science Center, Peking University, Beijing, People’s Republic of China

    Dagong Sun & Weibo Ka

  3. Department of Anatomy and Histology, Health Science Center, Peking University, Beijing, People’s Republic of China

    Guotao Wang, Jun Zhan, Yan Tang & Junming Tang

  4. Department of Bioengineering and Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla, CA

    Shu Chien

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  1. Zhu Zeng
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  10. Yan Tang
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Correspondence to Zongyao Wen.

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These authors contributed equally to this work.

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Zeng, Z., Liu, X., Jiang, Y. et al. Biophysical studies on the differentiation of human CD14+ monocytes into dendritic cells. Cell Biochem Biophys 45, 19–30 (2006). https://doi.org/10.1385/CBB:45:1:19

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