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Development of cytochrome-c oxidase activity in rat heart

Downregulation in newborn rats

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Abstract

Cytochrome-c oxidase (COX) activity of the rat heart was two- to sevenfold activated when the membrane integrity was disrupted by hypotonic swelling, freezing-thawing, or a detergent, indicating that a large portion of COX capacity in intact mitochondria is not active. The effect of detergent was tested in heart mitochondria isolated from 1-, 5-, 15-, 29-, and 60-d-old rats; activation by detergent was up to 20-fold in 1-d-old animals, whereas in mitochondria from 60-d-old rats it was only 7-fold. Our data indicate that the rat heart exhibits significant developmental changes dependent on downregulation of COX activity in the neonatal period.

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Authors and Affiliations

  1. Center for Integrated Genomics, Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20, Prague 4, Czech Republic

    Z. Drahota & J. Houstek

  2. Center for Cardiovascular Research, Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20, Prague 4, Czech Republic

    M. Milerova, A. Stieglerova, L. Skarka & B. Ostadal

Authors
  1. Z. Drahota
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  2. M. Milerova
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  3. A. Stieglerova
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  4. L. Skarka
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  5. J. Houstek
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  6. B. Ostadal
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Correspondence to B. Ostadal.

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Drahota, Z., Milerova, M., Stieglerova, A. et al. Development of cytochrome-c oxidase activity in rat heart. Cell Biochem Biophys 43, 87–94 (2005). https://doi.org/10.1385/CBB:43:1:087

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