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Modulatory effect of copper on nonenzymatic antioxidants in freshwater fish Channa punctatus (Bloch.)

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Abstract

Effect of the low level of copper exposure on nonenzymatic antioxidants was studied in a freshwater fish Channa punctatus (Bloch.). Fish were exposed to cupric chloride at the concentration of 10 ppb for 4 wk (28 d) in a static culture condition. Copper significantly (p < 0.001) increased the serum ceruloplasmin level and total iron-binding capacity. A significant (p < 0.05) increase in reduced glutathione level was recorded in all of the tissues. With regard to nonprotein thiols, copper decreased their level in the liver, but increased it in the gill. The protein-bound thiols remained unaltered except for an increase in the liver. Metallothionein (MT) induction was observed in liver only. Copper exposure had no significant effect on the ascorbic acid level and induced no lipid peroxidation over control values. It is suggested that by modulating the ceruloplasmin level, copper indirectly protects the fish, as it facilitates conversion of pro-oxidant iron to nonoxidant iron. It also induces an array of antioxidants that may be beneficial to fish in the case of oxidative stress resulting from chemical pollutants.

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Author notes

  1. Sheikh Raisuddin

    Present address: Department of Neurology, University of Magdeburg, Leipziger Str., 44d-39120, Magdeburg, Germany

  2. Iqbal Ahmad

    Present address: Departmento de Biologia, Universidade de Aveiro, 3800, Aveiro, Portugal

Authors and Affiliations

  1. Department of Medical Elementology and Toxicology, Hamdard University, 110062, New Delhi, India

    Suhel Parvez, Iqbal Sayeed, Suwarna Pandey, Bilal Bin-Hafeez, Rizwanul Haque, Iqbal Ahmad & Sheikh Raisuddin

  2. Department of Biochemistry, Jamia Hamdard, Hamdard University, 110062, New Delhi, India

    Ausaf Ahmad

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Parvez, S., Sayeed, I., Pandey, S. et al. Modulatory effect of copper on nonenzymatic antioxidants in freshwater fish Channa punctatus (Bloch.). Biol Trace Elem Res 93, 237–248 (2003). https://doi.org/10.1385/BTER:93:1-3:237

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  • DOI: https://doi.org/10.1385/BTER:93:1-3:237

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