Abstract
The effect of Na2SeO3 on the growth of Halobacterium halobium R1 was investigated by means of microcalorimetry at 37°C. The biological response to toxicants is observed as the inhibition of the rate constant of growth of living cells. A low concentration of Na2SeO3 stimulated the growth of H. halobium R1, and a high concentration of Na2SeO3 inhibited the growth of H. halobium R1. Toxicity may be expressed as the half-inhibition concentration (IC50). The rate constants of growth (k) and the concentrations of Na2SeO3 (c) shows a linear relationship: k=1.790 × 10−6 − 2.27 × 10−3 c. The value of IC50 obtained from the accompanying figure of I-c is 679 µg/mL.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Y. L. Wang, Advances of studies of bioselenium, Prog. Biochem. Biophys. 6, 28–34 (1987).
W. H. Wu and F. Y. Yang, Studies of selenium and membranes II. Protection of selenium to erythrocyte membranes, Sci. China (B) 4, 401–406 (1986).
E. Frieden, Biochemistry of Selenium, Plenum, London (1983).
X. Li, Y. Liu, F. J. Deng, and S. S. Qu, Microcalorimetric study of the toxic effect of selenite on the mitochondrial metabolism of Carassius auratus liver, Biol. Trace Element Res. 77, 261–271 (2000).
X. Li, Y. Liu, R. M. Zhao, J. Wu, X. S. Shen, and S. S. Qu, Microcalorimetric study of E. coli growth inhibited by the selenomorpholine complexes, Biol. Trace Element Res. 75, 167–175 (2000).
A. E. Beezer and B. Z. Chowdhry, Biological Microcalorimetry, Academic, London (1980).
E. Calvet and H. Prat, Recent Progress in Microcalorimetry, Pergamon, Oxford (1963).
S. M. McGuinness and B. G. Barisas, Actue toxicity measurement on aquatic pollutants using microcalorimetry on tissue-cultured cells, Environ. Sci. Technol. 25, 1098–1103 (1991).
R. A. Herbert and R. J. Sharp, Molecular Biology and Biotechnology of Extremophiles, Chapman & Hall, New York (1992).
R. A. Garrett, The uniqueness of Archaebacteria, Nature 318, 233–235 (1985).
C. L. Xie, H. K. Tang, Z. H. Song, and S. S. Qu, Microcalorimetric study of bacterial growth, Thermochim. Acta 123, 33–41 (1988).
R. Holzel, C. Motzkus, and I. Lamprecht, Kinetic investigations of microbial metabolism by means of flow calorimeters, Thermochim. Acta 239, 17–32 (1994).
J. Wu, Studies on the synthesis and properties of plant systemic organoselena compounds, PhD thesis, Wuhan University, Wuhan, People’s Republic of China (1998).
E. Schmolz, R. Doebner, R. Auste, R. Daum, G. Welge, and I. Lamprecht, Bioenergtic investigations on tea-tree and related essential soils, Thermochim. Acta 337, 71–81 (1999).
P. Weppen and D. Schuller, Microcalorimetric studies of the mode of action of environmental chemicals on continuous microbial cultures, Thermochim. Acta 72, 95–102 (1984).
S. M. McGuinness, D. A. Roess, and B. G. Barisas, Actue toxicity effects of mercury and other heavy metals on HeLa cells and human lymphocytes evaluated via microcalorimetry, Thermochim. Acta 172, 131–145 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Liu, Y., Yu, HG., Huang, YP. et al. Microcalorimetric studies of the action of Na2SeO3 on the growth of Halobacterium halobium R1. Biol Trace Elem Res 92, 83–91 (2003). https://doi.org/10.1385/BTER:92:1:83
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1385/BTER:92:1:83