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Analysis of zinc and magnesium levels in pinealectomized chicks

Roles on development of spinal deformity?

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Abstract

Melatonin is the main product of the pineal gland, and trace metals play a critical role in growth and development. The purpose of this study was to assess the serum zinc (Zn) and magnesium (Mg) levels in pinealec-tomized chicks and their possible interactions with the development of spinal deformity. Chicks were divided into two equal groups: unoperated controls (group M) and pinealectomized chicks (group N). Pinealectomies were performed at the age of 3 d. After 8 wk, serum Zn and Mg levels of 10 animals from each group were measured by spectrophotometric assay. The results of analyses were compared using the Mann-Whitney U-test. The correlation between serum Zn and Mg levels were assessed by Spearman's correlation. In this study, it was obvious that the serum Zn levels in group N were significantly lower than those in group M (2.8±0.10 vs 4.2±0.14 ppm; p<0.0005). In contrast, Mg levels in group N was high compared with the values in group M, although there was no significant difference (17.8±0.69 vs 15.7±0.85 ppm; p>0.05). In pinealectomized animals, serum Zn levels declined significantly while serum Mg levels increased, albeit insignificantly. Thus, there was a moderately positive but not statistically significant correlation between Mg and Zn levels in unoperated controls (r=0.273, p>0.05), whereas there was a negative but not statistically significant correlation between Zn and Mg levels in pinealectomized chicks (r=−0.115, p>0.05). In addition, the serum Mg to serum Zn ratio was significantly higher in group N than in the group M control (6.39±0.32 vs 3.75±0.22, respectively; p<0.001). From the results of the current study, it is clear that surgical pinealectomy in newly hatched Hybro Broiler chicks has a significant effect on serum Zn level. However, the serum Mg did not change significantly. Because serum Mg is not a good indicator of Mg status in chicks, it is speculated that other tissues, such as muscle or spine, might productively be explored as a more sensitive Mg biomarker for this model. The present study provides experimental evidence that serum trace metal levels might be affected in pinealectomized animals because of the lack of its main neurohormone melatonin.

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References

  1. K. Bagnall, V. J. Raso, M. Moreau, J. Mahood, X. Wang, and J. Zhao, The effects of melatonin therapy on the development of scoliosis after pinealectomy in the chicken, J. Bone Joint Surg. 81-A, 191–199 (1999).

    Google Scholar 

  2. M. Beuerlein, J. Wilson, M. Moreau, et al., The critical stage of pinealectomy surgery after which scoliosis is produced in young chickens, Spine 26, 237–240 (2001).

    Article  PubMed  CAS  Google Scholar 

  3. M. Machida, Cause of idiopathic scoliosis, Spine 24, 2576–2583 (1999).

    Article  PubMed  CAS  Google Scholar 

  4. M. Turgut, M. Ç. Yenisey, A. Uysal, M. Bozkurt, and M. E. Yurtseven, The effects of pineal gland transplantation on the production of spinal deformity and serum melatonin level following pinealectomy in the chicken, Eur. Spine J. 12, 487–494 (2003).

    Article  PubMed  Google Scholar 

  5. X. Wang, M. Moreau, J. Raso, et al., Changes in serum melatonin levels in response to pinealectomy in the chicken and its correlation with development of scoliosis, Spine 23, 2377–2381 (1998).

    Article  PubMed  CAS  Google Scholar 

  6. E. J. Underwood, Trace Elements in Human and Animal Nutrition, Academic, New York (1977).

    Google Scholar 

  7. G. Cikim, H. Canatan, M. F. Gursu, F. Gulcu, G. Baydas, and A. E. Kilicoglu, Levels of zinc and lipid peroxiadation in acute coronary syndrome, Biol. Trace Element Res. 96, 61–68 (2003).

    Article  CAS  Google Scholar 

  8. S. Michelle, P. Alain, and B. Françoise, Minerals, trace elements and related biological variables in athlets and during physical activity, Clin. Chim. Acta 321, 1–11 (2001)

    Google Scholar 

  9. G. Öztürk, Ş. Coşkun, D. Erbaş, and E. Hasanoglu, Effect of melatonin treatment on serum and tissue zinc levels in rats, J. Trace Elements Exp. Med. 15, 1–8 (2002).

    Article  CAS  Google Scholar 

  10. C. Wallwork, Zinc and central nervous system, Prog. Food Nutr. Sci. 11, 203–247 (1987).

    PubMed  CAS  Google Scholar 

  11. S. Yazar, E. Kiliç, and R. Saraymen, Changes of total content of magnesium and zinc status in patients with chronic toxoplasmosis, Biol. Trace Element Res. 92, 11–16 (2003).

    Article  CAS  Google Scholar 

  12. B. Poeggeler, R. J. Reiter, D. X. Tan, L. D. Chen, and L. C. Manchester, Melatonin, hydroxyl radical-mediated oxidative damage, and aging: a hypothesis J. Pineal Res. 14, 151–168 (1993).

    Article  PubMed  CAS  Google Scholar 

  13. A. S. King, and J. McLelland, Birds: Their Structure and Function, Bailliere Tindall, London, pp. 210–211 (1984).

    Google Scholar 

  14. R. J. Reiter, Pharmacological actions of melatonin in oxygen radical pathophysiology, Life Sci., 60, 2255–2271 (1997).

    Article  PubMed  CAS  Google Scholar 

  15. Q. I. Wan, and S. F. Pang, Segmental, colonal and subcellular distribution, of 2-(1251)Iodo melatonin binding sites in the chicken spinal cord, Neurosci. Lett. 180, 253–256 (1994).

    Article  PubMed  CAS  Google Scholar 

  16. R. J. Maughan, Role of micronutrients in sport and physical activity, Br. Med. Bull., 55, 683–690 (1999).

    Article  PubMed  CAS  Google Scholar 

  17. P. P. N. Lee, and S. F. Pang, Identification and characterization of melatonin binding sites in the gastrointestinal tract of duct, Life Sci. 50, 117–125 (1992).

    Article  PubMed  CAS  Google Scholar 

  18. M. T. Martin, F. Azpiroz, and J. R. Malagelada, Melatonin and the gastrointestinal tract, Therapie 53, 453–458 (1998).

    PubMed  CAS  Google Scholar 

  19. S. C. Cunnane, D. F. Horrobin, M. S. Manku, and M. Oka, Alteration of tissue zinc distribution and biochemical analysis of serum following pinealectomy in the rat, Endocr. Res. Commun. 6, 311–319 (1979).

    Article  PubMed  CAS  Google Scholar 

  20. J. Limson, T. Nyokong, and S. Daya, The interaction of melatonin and its precursors with aluminium, cadmium, copper, iron, lead, and zinc: an adsorptive voltammetric study, J. Pineal Res. 24, 15–21 (1998).

    Article  PubMed  CAS  Google Scholar 

  21. C. P. Mahoney, F. A. Alster, and L. B. J. Carew, Growth, thyroid function, and serum macromineral levels in magnesium-deficient chicks, Poult. Sci. 71, 1669–1679 (1992).

    PubMed  CAS  Google Scholar 

  22. M. Turgut, S. Kaplan, A. T. Turgut, et al., Morphological, stereological and radiological changes in pinealectomized chicken cervical vertebrae, J. Pineal Res. 39, 392–399 (2005).

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Neurosurgery College of Medicine, Adnan Menderes University, Aydin, Turkey

    Mehmet Turgut

  2. Department of Biochemistry, College of Medicine, Adnan Menderes University, Aydin, Turkey

    Çiĝdem Yenisey & Tuncay Biçakçi

  3. Department of Public Health, College of Medicine, Adnan Menderes University, Aydin, Turkey

    Filiz A. Ergin

  4. Institute of Agricultural Research of Erbeyli, Aydin, Turkey

    Mehmet Bozkurt

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  1. Mehmet Turgut
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  2. Çiĝdem Yenisey
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  3. Mehmet Bozkurt
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  4. Filiz A. Ergin
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  5. Tuncay Biçakçi
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Turgut, M., Yenisey, Ç., Bozkurt, M. et al. Analysis of zinc and magnesium levels in pinealectomized chicks. Biol Trace Elem Res 113, 67–75 (2006). https://doi.org/10.1385/BTER:113:1:67

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  • DOI: https://doi.org/10.1385/BTER:113:1:67

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