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Effects of acute manganese chloride exposure on lipid peroxidation and alteration of trace metals in rat brain

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Abstract

Although manganese (Mn) is an essential element, exposure to excessive levels of Mn and its accumulation in the brain can cause neurotoxicity and extrapyramidal syndrome. We have investigated the differences in the accumulated levels of Mn, the degree of lipid peroxidation, and its effects on the levels of trace elements (Fe, Cu, and Zn) in various regions in the brain of rats having undergone acute Mn exposure. The rats in the dose—effect group were injected intraperitoneally (ip) with MnCl2 (25, 50, or 100 mg MnCl2/kg) once a day for 24 h. The Mn significantly accumulated (p<0.05) in the frontal cortex, corpus callosum, hippocampus, striatum, hypothalamus medulla, cerebellum, and spinal cord in each case. The rats in the timecourse group were ip injected with MnCl2 (50 mg MnCl2/kg) and then monitored 12, 24, 48, and 72 h after exposure. The Mn accumulated in the frontal cortex, corpus callosum, hippocampus, striatum hypothalamus, medulla, cerebellum, and spinal cord after these periods of time, In both the dose—effect and time-course studies, we observed that the concentration of malondialdehyde, an end product of lipid peroxidation, increased significantly in the frontal cortex, hippocampus, striatum, hypothalamus, medulla, and cerebellum. However, no relationship between the concentrations of Mn in the brain and the extent of lipid peroxidation was observed. In addition, we found that there was a significant increase (p<0.05) in the level of Fe in the hippocampus, striatum, hypothalamus, medulla, and cerebellum, but the Cu and Zn levels had not changed significantly. These findings indicated that Mn induces an increase in the iron level, which provides direct evidence for Fe-mediated lipid peroxidation in the rats' brains; these phenomena might play important roles in the mechanisms of Mn-induced neurotoxicology.

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Authors and Affiliations

  1. Department of Medical Technology, Chung Hwa College of Medical Technology, Tainan, Taiwan

    Min-Tzu Chen

  2. Graduate Institute of Pharmacology, Pington, Taiwan

    Guan-Wen Cheng

  3. Faculty of Medicine, Pington, Taiwan

    Bai-Hsiun Chen

  4. Faculty of Biomedical Laboratory Science, Kaohsiung Medical University, Kaohsiung, Taiwan

    Yeod-Lih Huang

  5. Department of Laboratory Medicine, Fooyin University Hospital, Pington, Taiwan

    Ching-Chiang Lin

  6. Basic Medical Science Education Center, Fooyin University, Pingtong, Taiwan

    Ching-Chiang Lin

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Chen, MT., Cheng, GW., Lin, CC. et al. Effects of acute manganese chloride exposure on lipid peroxidation and alteration of trace metals in rat brain. Biol Trace Elem Res 110, 163–177 (2006). https://doi.org/10.1385/BTER:110:2:163

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  • DOI: https://doi.org/10.1385/BTER:110:2:163

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