Abstract
Zinc (Zn) is an essential nutrient that is required in humans and animals for many physiological functions, including immune and antioxidant function, growth, and reproduction. The present study was performed to investigate the effects of three Zn levels, including Zn adequate (35.94 mg/kg, as a control), Zn deficiency (3.15 mg/kg), and Zn overload (347.50 mg/kg) in growing male rats for 6 wk. This allowed for evaluation of the effects that these Zn levels might have on body weight, organ weight, enzymes activities, and tissues concentrations of Zn and Cu. The results showed that Zn deficiency has negative effects on growth, organ weight, and biological parameters such as alkaline phosphatase (ALP) and Cu−Zn superoxide dismutase (Cu−Zn SOD) activities, whereas Zn overload played an effective role in promoting growth, improving the developments of organs and enhancing immune system. Hepatic metallothionein (MT) concentration showed an identical increase tendency in rats fed both Zn-deficient and Zn-overload diets. The actual mechanism of reduction of Cu concentration of jejunum in rats fed a Zn-overload diet might involve the modulation or inhibition of a Cu transporter protein by Zn and not by the induction of MT.
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B. L. Vallee and K. H. Falchuk, The biochemical basis of zinc physiology, Physiol. Rev. 73, 79–118 (1993).
S. Chan, B. Gerson, and S. Subramaniam, The role of copper, molybdenum, selenium, and zinc in nutrition and health, Clin. Lab. Med. 18, 673–685 (1998).
H. A. El Hendy, M. I. Yousef, and N. I. Abo El-Naga, Effect of dietary zinc deficiency on hematological and biochemical parameters and concentrations of zinc, copper, and iron in growing rats, Toxicology 167, 163–170 (2001).
M. I. Yousef, H. A. El Hendy, F. M. El-Demerdash, et al., Dietary zinc deficiency-induced changes in the activity of enzymes and levels of free radicals, lipids and protein electrophoretic behavior in growing rats, Toxicology 175, 223–234 (2002).
I. Bremner, Nutritional and physiological significance of metallothionein, Experientia 52(Suppl.), 81–107 (1987).
W. P. Michael, B. Gerstmayer, A. Bosio, et al., Effect of zinc deficiency on the mRNA expression pattern in liver and jejunum of adult rats: monitoring gene expression using cDNA micoroarrays combined with real-time RT-PCR, J. Nutr. Biochem. 14, 691–702 (2003).
N. F. Shay and H. F. Manigan, Neurobiology of zinc-influenced eating behavior, J. Nutr. 130, 1493S-1499S (2000).
D. Carlson, H. D. Poulsen, and J. Sehested, Influence of weaning and effect of post weaning dietary zinc and copper on electrophysiological response to glucose, theophylline and 5-HT in pignet small intestinal mucosa, Comp. Biochem. Physiol. A. 137(4), 757–765 (2004).
J. W. Smith, M. D. Tokach, R. D. Goodmand, et al., Effects of the interrelationship between zinc and copper sulfate on growth performance of early-weaned pigs, J. Anim. Sci. 75, 1861–6 (1997).
J. Szabó, M. Hegedus, G. Bruckner, et al. Large doses of zinc increases the activity of hydrolases in rats. J. Nutr. Biochem. 15, 206–209 (2004).
A. E. Favier, The role of zinc in reproduction: hormonal mechanisms, Biol. Trace Element Res. 32, 363–382 (1992).
J. C. Welkell, K. D. Shearer, and E. J. Gauglitz III, Zinc supplementation of trout diets: tissue indicators of body zinc status, Prog. Fish-Cult. 48, 205–212 (1986).
S. I. Kettler, K. Eder, A. Kettler, et al., Zinc deficiently and the activities of lipoprotein lipase in plasma and tissues of rats force-fed diets with coconut oil or fish oil, J. Nutr. Biochem. 11, 132–138 (2000).
H. T. Dieck, F. Doring, H. P. Roth, et al., Changes in rat hepatic gene expression in response to zinc deficiency as assessed by DNA arrays, J. Nutr. 133, 1004–1010 (2003).
N. W. Tietz, A. D. Rinker and L. M. Shaw, IFCC methods for the measurement of catalytic concentrations of enzymes. Part 5: IFCC methods for alkaline phosphatase. J. Clin. Chem. Clin. Biochem. 21, 731–748 (1983).
F. Mahmoodian, A. Gosiewska, and B. Peterkofsky, Regulation and properties of bone alkaline phosphatase during vitamin C deficiency in guinea pigs, Arch. Biochem. Biophys. 336(1), 86–96 (1996).
Y. Sun, L. W. Oberley, and Y. Li, A simple method for clinical assay of superoxide dismutase. Clin. Chem. 34, 497–500 (1988).
D. L. Eaton and B. F. Toal, Evaluation of the Cd/hemoglobin affinity assay for the rapid determination of metallothionein in biological tissues Toxicol. Appl. Pharmacol. 66, 134–142 (1982).
M. S. Clegg, C. L. Keen, and B. Lonnerdal, Influence of ashing techniques on the analysis of trace elements in animal tissues, Biol. Trace Element Res. 3, 107–115 (1981).
R. G. D. Steel and J. H. Torrie, Principle and procedure of statistics, in A Biochemical Approach, 2nd ed., McGraw-Hill, New York (1980).
A. S. Prasad, Biochemistry of Zinc, Plenum, New York (1993).
H. Ai, J. Chen, and S. He, The effects of zinc deficiency and testosterone supplement on testosterone synthesis and skeletal muscle of rats. Wei Sheng Yen Chiu 26, 211–215 (1997).
A. Kraus, H. Roth, and M. Kirchgessner, Supplementation with vitamin C, vitamin E or β-carotene influences osmotic fragility and oxidative damage of erythrocytes of zinc-deficient rats, J. Nutr. 127, 1290–1296 (1997).
R. S. MacDonald, L. C. Wollard-Biddle, J. D. Browning, et al., Zinc deprivation of murine 3T3 cells by use of diethylenetrinitrilopentaacetate impairs DNA synthesis upon stimulation with insulin-like growth factor-I (IGF-I), J. Nutr. 128, 1600–1605 (1998).
G. Levin, U. Cogan, and S. Mokady, Food restriction and membrane fluidity, Mech. Aging Dev. 62, 137–141 (1992).
W. B. Essman, Perspective for nutrients and brain function, in Nutrients and Brain Function, A. G. Karger, Switzerland, pp. 1–10 (1987).
G. B. Martin and C. L. White, Effects of dietary zinc deficiency on gonadotrophin secretion and testicular growth in young male sheep. J. Reprod. Fertil 96, 497–507 (1992).
L. Stallard and P. G. Reeves, Zinc deficiency in adult rats reduces the relative abundance of testis-specific angiotensin-converting enzyme mRNA, J. Nutr. 127, 25–29 (1997).
E. J. Underwood, The mineral nutrition of livestock, Oxford University Press, UK. (1981).
T. Ogiso, K. Mariyama, and S. Sasaki, Inhibitory effect of high zinc on copper absorption in rats, Chem. Pharm. Bull. 22, 55–60 (1974).
T. Ogiso, N. Ogawa, and T. Miura, Inhibitory effect of dietary zinc on copper absorption in rats, II. Binding of copper and zinc to cytosol proteins in the intestinal mucosa, Chem. Pharm. Bull. 27, 515–521 (1979).
P. W. F. Fischer, A. Giroux, and M. R. L. Abbe, Effects of zinc on mucosal copper binding and on the kinetics of copper absorption, J. Nutr. 113, 462–469 (1983).
P. G. Reeves, Copper metabolism in metallothionein-null mice fed a high-zinc diet, J. Nutr. Biochem. 9, 598–601 (1998).
P. G. Reeves, Adaptation responses in rats to long-term feeding of high-zinc diets: emphasis on intestinal metallothionein, J. Nutr. Biochem. 6, 48–54 (1995).
P. G. Reeves, Copper status of adult male rats is not affected by feeding an AIN-93G-based diet containing high concentrations of zinc, J. Nutr. Biochem. 7, 166–172 (1996).
I. Bremner and N. T. Davies, The induction of metallothionein in rat liver by zinc injection and restriction of food intake, Biochem. J. 149, 733–738 (1975).
M. P. Richards, and R. J. Cousins, Metallothionein and its relationship to the metabolism of dietary zinc in rats, J. Nutr. 106, 1591–1599 (1976).
I. E. Dreosti, Zinc and the gene, Mutat. Res. 475, 161–167 (2001).
I. Bremner, W. G. Hoekstra, N. T. Davies, et al., Effect of zinc status of rats on the synthesis and degradation of copper-induced metallothioneins, Biochem. J. 174, 883–892 (1978).
B. Sas and I. Bremner, Effect of acute stress on the absorption and distribution of zinc and on Zn-metallothionein production in the liver of the chick, J. Inorg. Biochem. 11, 67–76 (1979).
A. I. Alayash, Zinc and some zinc dependent enzymes in sickle cell anemia, Int. J. Vitam. Nutr. Res. 59(4), 388–389 (1989).
R. Lakshmi, R. Kundu, E. Thomas, et al., Mercuric chloride induced inhibition of acid and alkaline phosphatase activity in the kidney of mudskipper; Boleophthalmus dentatus, Acta Hydrochim. Hydrobiol. 3, 341–344 (1991).
J. E. Coleman, Structure and metabolism of alkaline phosphatase, Annu. Rev. Biomol. Struct. 21, 441–483 (1992).
F. Anna, E. R. Miller, and P. K. Ku, Effect of elevated dietary zinc on growth performance of weaning swine, Michigan State Univ. Rep. Swine Res. 520, 128–132 (1992).
G. M. Hill and E. R. Miller, Effect of dietary zinc livels on the growth and development of the gilt, J. Anim. Sci. 57, 106–113 (1983).
Z. R. Xu and M. Q. Wang, Approach of the mechanism of growth promoting effect of pharmacological level of zinc in pigs. Acta, Vet. Zootech. Sin. 32(1), 11–17 (2001).
J. K. Chesters, Biochemistry of zinc in cell division and tissue growth, in Zinc in Human Biology, C. F. Mills, eds., Springer-Verlag, London, pp. 109–118 (1989).
A. S. Prasad, F. W. Beck, L. Endre, et al., Zinc deficiency affects cell cycle and deoxythymidine kinase gene expression in HUT 78 cells, J. Lab. Clin. Med. 128, 51–60 (1996).
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Sun, J.Y., Jing, M.Y., Weng, X.Y. et al. Effects of dietary zinc levels on the activities of enzymes, weights of organs, and the concentrations of zinc and copper in growing rats. Biol Trace Elem Res 107, 153–165 (2005). https://doi.org/10.1385/BTER:107:2:153
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DOI: https://doi.org/10.1385/BTER:107:2:153