Abstract
Oxygen availability is the most important environmental parameter in the production of xylitol by yeasts, directly affecting yields and volumetric productivity. This work evaluated the cell behavior in fermentations carried out with different dissolved oxygen concentrations (0.5–30.0% of saturation), as well as a limited oxygen restriction (0% of saturation), at several oxygen volumetric transfer coefficients (12 ≤ k L a ≤ 70 h−1). These experiments allowed us to establish the specific oxygen uptake rate limits to ensure high yields and volumetric productivity. When oxygen availability was limited, the specific oxygen uptake rate values were between 12 and 26 mg of O2/of g cell·h, resulting in a yield of 0.71 g of xylitol/xylose consumed, and 0.85 g/[L·h] for the volumetric productivity. According to the results, the effective control of the specific oxygen uptake rate makes it possible to establish complete control over this fermentative process, for both cell growth and xylitol production.
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Abbreviations
- C ox *:
-
saturated DO concentration (mg of O2/L)
- C ox :
-
DO concentration (mg of O2/L)
- k L a :
-
volumetric oxygen transfer coefficient h−1
- P:
-
product: Extracellular xylitol (g/L)
- q O 2 :
-
specific oxygen uptake rate, mg O2 / (g cell·h)
- S :
-
substrate: xylose (g/L)
- X :
-
cells (g/L)
- Y P/S :
-
xylitol yield coefficient (g of xylitol/g of xylose)
- Y X/S :
-
cell mass yield coefficient (g of cells/g of xylose)
References
Barbosa, M. F. S., Medeiros, M. B., Mancilha, I. M., Schneider, H., and Lee, H. (1988), J. Ind. Microbiol. 3, 241–251.
Silva, S. S. (1994), DS thesis, University of São Paulo, São Paulo, Brazil.
Sá, M.C.A. (1993), MS thesis, Federal University of Rio de Janeiro/EQ, Rio de Janeiro, Brazil.
Horitsu, H., Yahasi, Y., Takamizawa, K., Kawai, K., Suzuki, T., and Watanabe, N. (1992), Biotechnol. Bioeng. 40, 1085–1091.
Nolleau, V., Preziosi-Belloy, L., and Navarro, J.M. (1995), Biotechnol. Lett. 17, 417–422.
Felipe, M. G. A., Vitolo, M., Mancilha, I. M., and Silva, S. S. (1997), J. Ind. Microbiol. & Biotechnol. 18, 251–254.
Du Preez, J. C. (1994), Enzyme Microb. Technol. 16, 944–956.
Aguiar, Jr, W. B. (1998), MS thesis, Federal University of Rio de Janeiro/EQ, Rio de Janeiro, Brazil.
Faria, L.F.F. (2000), DS. thesis, Federal University of Rio de Janeiro/COPPE, Rio de Janeiro, Brazil.
Hahn-Hargerdal, B., Jeppsson, H., Skoog, K., and Prior, B.A. (1994), Enzyme Microb. Technol. 16, 933–943.
Du Preez, J. C. and Van Der Walt, J. P. (1983), Biotechnol. Lett. 6, 395–400.
Pereira Jr., N. (1991), PhD thesis, The University of Manchester, UK.
Moser, A. (1988), Bioprocess Technology — Kinetics and Reactors, Springer-Verlag, NY.
Voet, D. and Voet, J. G. (1995), Biochemistry, John Wiley & Sons, NY.
Ojamo, H. (1994). DS thesis, Helsinki University of Technology, Espoo, Filand.
Faria, L. F. F., Pereira Jr., N., Nobrega, R., and Alves, T. (2000), Braz. J. Microbiol. 31, 73–78.
Silva, S. S., Vitolo, M., and Felipe, M. G. A. (1996), J. Basic Microbiol. 36(3), 187–191.
Winkelhausen, E., Pittman, P., Kuzmanova, S., and Jeffries, T. W. (1996), Biotechnol. Lett. 18, 753–758.
Stanbury, P. F., Whitake, A., and Hall, S. J. (1995), Principles of Fermentation Technology, 2nd ed., Elsevier Science, NY.
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Faria, L.F.F., Gimenes, M.A.P., Nobrega, R. et al. Influence of oxygen availability on cell growth and xylitol production by Candida guilliermondii . Appl Biochem Biotechnol 98, 449–458 (2002). https://doi.org/10.1385/ABAB:98-100:1-9:449
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DOI: https://doi.org/10.1385/ABAB:98-100:1-9:449